Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0585.Seq
(492 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59520.1 68416.m06642 rhomboid family protein contains Pfam p... 32 0.18
At4g14970.1 68417.m02301 hypothetical protein 31 0.42
At1g11480.1 68414.m01319 eukaryotic translation initiation facto... 30 0.74
At1g06960.2 68414.m00741 small nuclear ribonucleoprotein U2B, pu... 29 1.3
At1g06960.1 68414.m00740 small nuclear ribonucleoprotein U2B, pu... 29 1.3
At5g03490.1 68418.m00305 UDP-glucoronosyl/UDP-glucosyl transfera... 29 2.2
At3g30550.1 68416.m03866 hypothetical protein includes At2g05890... 29 2.2
At2g30260.1 68415.m03684 small nuclear ribonucleoprotein U2B, pu... 29 2.2
At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 29 2.2
At4g14280.1 68417.m02201 hypothetical protein 28 3.0
At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa s... 28 3.0
At1g03230.1 68414.m00301 extracellular dermal glycoprotein, puta... 28 3.0
At2g30670.1 68415.m03740 tropinone reductase, putative / tropine... 28 3.9
At1g76090.1 68414.m08836 S-adenosyl-methionine-sterol-C-methyltr... 27 5.2
At4g35210.1 68417.m05004 hypothetical protein contains Pfam prof... 27 6.9
At1g65470.1 68414.m07427 chromatin assembly factor-1 (FASCIATA1)... 27 6.9
At1g67460.1 68414.m07681 hypothetical protein contains Pfam doma... 27 9.1
>At3g59520.1 68416.m06642 rhomboid family protein contains Pfam
profile PF01694: Rhomboid family
Length = 269
Score = 32.3 bits (70), Expect = 0.18
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = -2
Query: 392 LSLSTALSPFEILSFTELVIPHVPAFFGHL 303
LSL + +PFE L FT +++P +F GHL
Sbjct: 158 LSLPISFAPFESLIFTSIIVPQA-SFLGHL 186
>At4g14970.1 68417.m02301 hypothetical protein
Length = 1286
Score = 31.1 bits (67), Expect = 0.42
Identities = 21/81 (25%), Positives = 35/81 (43%)
Frame = +1
Query: 55 SEVNEKMKREDLKDALTKLGAEVAYIDFKSGDTEGWVRLSKENAAKEMVEKIEDGKIKIC 234
S+VNEK+++ + DA K GA +++ + T + AA M E D + I
Sbjct: 718 SDVNEKLRQPTIMDAFKKAGAVMSHSQTQLRGTPSLPSMDGSTAAGSMDENCSDNESLIV 777
Query: 235 DSEVIFNCLKVTMRKRISLKP 297
+ + L+ K L P
Sbjct: 778 KIPQVSSALEAQRFKFRPLLP 798
>At1g11480.1 68414.m01319 eukaryotic translation initiation
factor-related contains weak similarity to
Swiss-Prot:P23588 eukaryotic translation initiation
factor 4B (eIF-4B) [Homo sapiens]
Length = 578
Score = 30.3 bits (65), Expect = 0.74
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +1
Query: 280 RISLKPWKR*PKNAGT*GITSSVKERISKGDKAVERESPKIMKRHLQRKSKRSNQN 447
R+ LKP +R P++A ++S R K D+ R I + QR++ R N+N
Sbjct: 426 RMVLKPIERVPEHASQRPVSSPRDMRTRKFDQKDGRNVSDIARSETQRRNWRENEN 481
>At1g06960.2 68414.m00741 small nuclear ribonucleoprotein U2B,
putative / spliceosomal protein, putative non-consensus
splice donor GC at exon 4; similar to spliceosomal
protein (U2B) GI:169588 from [Solanum tuberosum]
Length = 228
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Frame = +1
Query: 28 LPTGSVFHFSEVNEKMKREDLKDAL----TKLGAEVAYIDFKSGDTEG--WVRLSKENAA 189
+P + +NEK+K+E+LK +L ++ G + + K+ G WV ++ AA
Sbjct: 6 IPPNQSIYIKHINEKIKKEELKRSLYCLFSQFGRLLDVVALKTPKLRGQAWVVFTEVTAA 65
Query: 190 KEMVEKIED 216
V ++++
Sbjct: 66 SNAVRQMQN 74
>At1g06960.1 68414.m00740 small nuclear ribonucleoprotein U2B,
putative / spliceosomal protein, putative non-consensus
splice donor GC at exon 4; similar to spliceosomal
protein (U2B) GI:169588 from [Solanum tuberosum]
Length = 229
Score = 29.5 bits (63), Expect = 1.3
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Frame = +1
Query: 28 LPTGSVFHFSEVNEKMKREDLKDAL----TKLGAEVAYIDFKSGDTEG--WVRLSKENAA 189
+P + +NEK+K+E+LK +L ++ G + + K+ G WV ++ AA
Sbjct: 6 IPPNQSIYIKHINEKIKKEELKRSLYCLFSQFGRLLDVVALKTPKLRGQAWVVFTEVTAA 65
Query: 190 KEMVEKIED 216
V ++++
Sbjct: 66 SNAVRQMQN 74
>At5g03490.1 68418.m00305 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 465
Score = 28.7 bits (61), Expect = 2.2
Identities = 22/65 (33%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -2
Query: 320 AFFGHLFHGFSEIRFLIVTFKQLKMTSESHILIFP-SSIFSTISLAAFSLDSLTQPSVSP 144
AFF F S ++F +K T H+L P + IF L + SL P SP
Sbjct: 149 AFFSISFFLVSVLQFCFENIDLIKSTDPIHLLDLPRAPIFKEEHLPSIVRRSLQTP--SP 206
Query: 143 DLKSM 129
DL+S+
Sbjct: 207 DLESI 211
>At3g30550.1 68416.m03866 hypothetical protein includes At2g05890,
At4g07450, At3g30630, At3g43100, At2g09960, At3g30550,
At1g39430, At2g10460, At4g03640, At5g35250
Length = 509
Score = 28.7 bits (61), Expect = 2.2
Identities = 19/82 (23%), Positives = 37/82 (45%), Gaps = 7/82 (8%)
Frame = -2
Query: 362 EILSFTELVIPHVPAFFGHLFHGF--SEIRFLIVTFKQLKMTSESHILIFPSSIFSTISL 189
E + F ++ +P P F+ + +GF S + + + T E+ ++F S + +
Sbjct: 312 EAIKFEDVKLPQKPRFWCEICNGFAKSVVPKFWLHLHIMDQTGEAKCMLFDSHAKEILGI 371
Query: 188 AAF-----SLDSLTQPSVSPDL 138
A S D + P+V PD+
Sbjct: 372 TAHDLLAGSFDEIEDPTVLPDV 393
>At2g30260.1 68415.m03684 small nuclear ribonucleoprotein U2B,
putative / spliceosomal protein, putative similar to
spliceosomal protein [Solanum tuberosum] GI:169589
Length = 232
Score = 28.7 bits (61), Expect = 2.2
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Frame = +1
Query: 28 LPTGSVFHFSEVNEKMKREDLKDAL----TKLGAEVAYIDFKSGDTEG--WVRLSKENAA 189
+P + +NE++K+E+LK +L ++ G + + K+ G WV S+ AA
Sbjct: 6 IPPNQSIYIQNLNERIKKEELKRSLYCLFSQFGRILDVVALKTPKLRGQAWVTFSEVTAA 65
Query: 190 KEMVEKIED 216
V ++++
Sbjct: 66 GHAVRQMQN 74
>At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profile PF00226: DnaJ domain
Length = 2554
Score = 28.7 bits (61), Expect = 2.2
Identities = 22/105 (20%), Positives = 49/105 (46%), Gaps = 4/105 (3%)
Frame = +1
Query: 1 QQQEKEELKLPTGSVFHFSEVN-EKMKREDL-KDALTKLGAEVAYID--FKSGDTEGWVR 168
++ ++ + L G E++ + +DL + A +L E++ I ++ D E R
Sbjct: 1486 EELTRKPMDLSEGEACKILEISLNNVSSDDLNRTASVELNEEISNISKQIQNLDEEKLKR 1545
Query: 169 LSKENAAKEMVEKIEDGKIKICDSEVIFNCLKVTMRKRISLKPWK 303
++ A + +K +G+ K + + CL+ TM+ +PW+
Sbjct: 1546 QYRKLAMRYHPDKNPEGREKFLAVQKAYECLQATMQGLQGPQPWR 1590
>At4g14280.1 68417.m02201 hypothetical protein
Length = 798
Score = 28.3 bits (60), Expect = 3.0
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +1
Query: 34 TGSVFHFSEVNEKMKREDLKDALTKLGAEV-AYIDFKSGDTE 156
+G VF S + E + + L KLGAE+ ++ F+ G TE
Sbjct: 515 SGIVFTVSNIRETLHHGKSQPHLQKLGAEILTFLAFEEGATE 556
>At4g02580.1 68417.m00352 NADH-ubiquinone oxidoreductase 24 kDa
subunit, putative similar to NADH-ubiquinone
oxidoreductase 24 kDa subunit, mitochondrial precursor
(EC 1.6.5.3) (EC 1.6.99.3) (Polypeptide II)
(Swiss-Prot:P04394) [Bos taurus]
Length = 255
Score = 28.3 bits (60), Expect = 3.0
Identities = 19/76 (25%), Positives = 33/76 (43%), Gaps = 2/76 (2%)
Frame = +1
Query: 46 FHFSEVNEKMKREDLK--DALTKLGAEVAYIDFKSGDTEGWVRLSKENAAKEMVEKIEDG 219
+ FSE N+ +E L + K A + +D GW+ +S NA +++E
Sbjct: 47 WEFSEANQSKVKEILSYYPSNYKQSAVIPLLDLAQQQNGGWLPVSAMNAVAKVIEVAPIR 106
Query: 220 KIKICDSEVIFNCLKV 267
++ +FN KV
Sbjct: 107 VYEVATFYSMFNRAKV 122
>At1g03230.1 68414.m00301 extracellular dermal glycoprotein,
putative / EDGP, putative similar to extracellular
dermal glycoprotein EDGP precursor [Daucus carota]
GI:285741
Length = 434
Score = 28.3 bits (60), Expect = 3.0
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -2
Query: 209 IFSTISLAAFSLDSLTQPSVSPDLKSMYATSAPNLVR 99
IFS + L+ FSL S QPS P + T P+ ++
Sbjct: 8 IFSVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQ 44
>At2g30670.1 68415.m03740 tropinone reductase, putative / tropine
dehydrogenase, putative similar to tropinone reductase
SP:P50165 from [Datura stramonium]
Length = 262
Score = 27.9 bits (59), Expect = 3.9
Identities = 18/63 (28%), Positives = 35/63 (55%)
Frame = -2
Query: 254 LKMTSESHILIFPSSIFSTISLAAFSLDSLTQPSVSPDLKSMYATSAPNLVRASFKSSLF 75
L SE +IF SS+ +S+ S+ SLT+ +++ K++ A + +RA+ + F
Sbjct: 132 LLKASEFGSIIFISSVGGVVSMECGSIYSLTKGALNQLAKTLACEWARDGIRANSVAPNF 191
Query: 74 IFS 66
I++
Sbjct: 192 IYT 194
>At1g76090.1 68414.m08836
S-adenosyl-methionine-sterol-C-methyltransferase
identical to
S-adenosyl-methionine-sterol-C-methyltransferase
GI:2246456 from [Arabidopsis thaliana]
Length = 359
Score = 27.5 bits (58), Expect = 5.2
Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 5/44 (11%)
Frame = +2
Query: 116 LRSHTLISNLVTPKVGSDCLK-----KTPLKKWLKRLKMERLRY 232
LRS+ I+ + KVG + +K K P K W RLKM R+ Y
Sbjct: 258 LRSYADIA-VTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAY 300
>At4g35210.1 68417.m05004 hypothetical protein contains Pfam profile
PF03087: Arabidopsis protein of unknown function
Length = 250
Score = 27.1 bits (57), Expect = 6.9
Identities = 31/118 (26%), Positives = 58/118 (49%), Gaps = 6/118 (5%)
Frame = +1
Query: 154 EGWVRLSKENAA--KEMVEKIEDGKIKICD----SEVIFNCLKVTMRKRISLKPWKR*PK 315
E +RLS N A ++ +EK+ DG IKI D S+ + +K ++++ S+ KR
Sbjct: 56 EKMIRLSVTNQALSQDQIEKLLDGSIKILDLCSISKDGLSQMKESLKEIQSIVRRKR--- 112
Query: 316 NAGT*GITSSVKERISKGDKAVERESPKIMKRHLQRKSKRSNQNSQTALRMFCASEAI 489
G S+ ++ K +++ K++ KS +++QN AL +F +E +
Sbjct: 113 -----GDLSAEVKKYLASRKFLKKSFEKVL------KSLKTSQNKNDALAVFGEAETV 159
>At1g65470.1 68414.m07427 chromatin assembly factor-1 (FASCIATA1)
(FAS1) identical to FAS1 [Arabidopsis thaliana]
GI:4887626
Length = 815
Score = 27.1 bits (57), Expect = 6.9
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +1
Query: 58 EVNEKMKREDLKDALTKLGAEVAYIDFKSGDTEGWVRLSKENAAKE 195
E EK+ R DL A KLG ++ +D +S + S E A K+
Sbjct: 195 EETEKLWRSDLSKAAEKLGKILSEVDIRSFMDNMMQKNSSEMAEKD 240
>At1g67460.1 68414.m07681 hypothetical protein contains Pfam domain
PF03193: Protein of unknown function, DUF258
Length = 434
Score = 26.6 bits (56), Expect = 9.1
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = +1
Query: 367 GDKAVERESPKIMKRHLQRKSKRSNQNSQTALR 465
G+K VE+ P+I + +R+S+++ ++ + LR
Sbjct: 395 GEKGVEQAKPRIDPKKYRRESRKTMKDERAILR 427
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,597,706
Number of Sequences: 28952
Number of extensions: 156697
Number of successful extensions: 653
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 643
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 652
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 858708096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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