BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0581.Seq (489 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g18220.1 68415.m02123 expressed protein contains Pfam domain ... 28 2.9 At4g12210.1 68417.m01938 zinc finger (C3HC4-type RING finger) fa... 27 5.1 At1g19860.1 68414.m02491 zinc finger (CCCH-type) family protein ... 27 6.8 At3g24780.1 68416.m03110 hypothetical protein 27 9.0 At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi do... 27 9.0 >At2g18220.1 68415.m02123 expressed protein contains Pfam domain PF03715: Uncharacterised protein family (UPF0120) Length = 779 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/52 (32%), Positives = 31/52 (59%) Frame = +3 Query: 45 DLTEDFLTNTLLSTGRKLGELFDIMPPIPVKNEDIVIEEVSDHETRNTTEVR 200 D+ EDF+ LS+ + +LFDI K+ED ++E++D ET+ + + + Sbjct: 713 DVVEDFV----LSSDEEEEDLFDIGGD---KDEDDAVDEIADPETKTSKKTK 757 >At4g12210.1 68417.m01938 zinc finger (C3HC4-type RING finger) family protein low similarity to RING-H2 finger protein RHG1a [Arabidopsis thaliana] GI:3822225; contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 203 Score = 27.5 bits (58), Expect = 5.1 Identities = 9/26 (34%), Positives = 18/26 (69%) Frame = +3 Query: 114 IMPPIPVKNEDIVIEEVSDHETRNTT 191 ++ I K+E+I + + +DH+TR +T Sbjct: 26 VIVEIDTKSEEIFVNQTTDHQTRRST 51 >At1g19860.1 68414.m02491 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 413 Score = 27.1 bits (57), Expect = 6.8 Identities = 9/28 (32%), Positives = 20/28 (71%) Frame = +3 Query: 114 IMPPIPVKNEDIVIEEVSDHETRNTTEV 197 ++P +PV+++DI ++ SD T++ +V Sbjct: 145 VIPILPVEDDDIAMDSASDFPTQSGVDV 172 >At3g24780.1 68416.m03110 hypothetical protein Length = 715 Score = 26.6 bits (56), Expect = 9.0 Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 1/43 (2%) Frame = +3 Query: 72 TLLSTGRK-LGELFDIMPPIPVKNEDIVIEEVSDHETRNTTEV 197 T LS+G L F I+P P K+ + +EE DH++ T ++ Sbjct: 119 TYLSSGNPCLDFFFHIVPSTPKKSLEQRLEEAWDHDSLTTLKL 161 >At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi domain-containing protein similar to SP|Q9XGW1 PINHEAD protein (ZWILLE protein) {Arabidopsis thaliana}; contains Pfam profiles PF02171: Piwi domain, PF02170: PAZ domain Length = 990 Score = 26.6 bits (56), Expect = 9.0 Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 4/62 (6%) Frame = -2 Query: 206 KTPDFGRVSGLVVGYLLNDDVFVFDGYRR---HNVEQFS*FPSRG-QQCVRQEILSEIRF 39 K PDFG G V+ L N + FD +R +NVE S PS+ + ++Q+++ R Sbjct: 152 KRPDFGGQDGSVIYLLANHFLVKFDSSQRIYHYNVE-ISPQPSKEIARMIKQKLVETDRN 210 Query: 38 DF 33 F Sbjct: 211 SF 212 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,333,090 Number of Sequences: 28952 Number of extensions: 114564 Number of successful extensions: 250 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 250 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 250 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 848837888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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