BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0521.Seq (449 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.) 111 3e-25 SB_32282| Best HMM Match : Laminin_G_2 (HMM E-Value=0) 27 5.4 >SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 252 Score = 111 bits (267), Expect = 3e-25 Identities = 57/78 (73%), Positives = 60/78 (76%), Gaps = 1/78 (1%) Frame = +3 Query: 219 TREHLLVF-FTNQEFEIIDFXLGPXLXDEVLKIMPVQKXTRAGQRXRFKAFVAIGXXNGH 395 T EH+ +F +EFEIIDF LG L DEVLKIMPVQK TRAGQR RFKAFVAIG NGH Sbjct: 25 TLEHIYLFSLPIKEFEIIDFFLGAALKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDSNGH 84 Query: 396 XGLGVKCSKEVAXXXRGA 449 GLGVKCSKEVA RGA Sbjct: 85 VGLGVKCSKEVATAIRGA 102 Score = 52.0 bits (119), Expect = 2e-07 Identities = 24/30 (80%), Positives = 26/30 (86%) Frame = +1 Query: 166 WVPVTKLGRLVREGKIXXLESIYLFSLPIK 255 WVPVTKLGRLV++ KI LE IYLFSLPIK Sbjct: 8 WVPVTKLGRLVKDLKIKTLEHIYLFSLPIK 37 >SB_32282| Best HMM Match : Laminin_G_2 (HMM E-Value=0) Length = 897 Score = 27.5 bits (58), Expect = 5.4 Identities = 10/30 (33%), Positives = 17/30 (56%) Frame = +3 Query: 342 GQRXRFKAFVAIGXXNGHXGLGVKCSKEVA 431 G++ + K F+A+G NGH C ++A Sbjct: 356 GRKDKRKDFLALGLRNGHVEFRFSCGADIA 385 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,872,423 Number of Sequences: 59808 Number of extensions: 97575 Number of successful extensions: 149 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 16,821,457 effective HSP length: 76 effective length of database: 12,276,049 effective search space used: 896151577 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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