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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0521.Seq
         (449 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.)              111   3e-25
SB_32282| Best HMM Match : Laminin_G_2 (HMM E-Value=0)                 27   5.4  

>SB_4413| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 252

 Score =  111 bits (267), Expect = 3e-25
 Identities = 57/78 (73%), Positives = 60/78 (76%), Gaps = 1/78 (1%)
 Frame = +3

Query: 219 TREHLLVF-FTNQEFEIIDFXLGPXLXDEVLKIMPVQKXTRAGQRXRFKAFVAIGXXNGH 395
           T EH+ +F    +EFEIIDF LG  L DEVLKIMPVQK TRAGQR RFKAFVAIG  NGH
Sbjct: 25  TLEHIYLFSLPIKEFEIIDFFLGAALKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDSNGH 84

Query: 396 XGLGVKCSKEVAXXXRGA 449
            GLGVKCSKEVA   RGA
Sbjct: 85  VGLGVKCSKEVATAIRGA 102



 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 24/30 (80%), Positives = 26/30 (86%)
 Frame = +1

Query: 166 WVPVTKLGRLVREGKIXXLESIYLFSLPIK 255
           WVPVTKLGRLV++ KI  LE IYLFSLPIK
Sbjct: 8   WVPVTKLGRLVKDLKIKTLEHIYLFSLPIK 37


>SB_32282| Best HMM Match : Laminin_G_2 (HMM E-Value=0)
          Length = 897

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 10/30 (33%), Positives = 17/30 (56%)
 Frame = +3

Query: 342 GQRXRFKAFVAIGXXNGHXGLGVKCSKEVA 431
           G++ + K F+A+G  NGH      C  ++A
Sbjct: 356 GRKDKRKDFLALGLRNGHVEFRFSCGADIA 385


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,872,423
Number of Sequences: 59808
Number of extensions: 97575
Number of successful extensions: 149
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 16,821,457
effective HSP length: 76
effective length of database: 12,276,049
effective search space used: 896151577
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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