Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0520.Seq
         (548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   105   4e-24
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   105   4e-24
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    67   1e-12
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    58   1e-09
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce...    31   0.11 
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu...    28   0.79 
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz...    27   1.8  
SPCC1259.06 |||transcription factor TFIID complex subunit 8 |Sch...    25   9.7  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  105 bits (252), Expect = 4e-24
 Identities = 54/84 (64%), Positives = 61/84 (72%)
 Frame = +2

Query: 35  LGKTGTIXTXKNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 214
           L K+GT+ T + AHNMKVMK S  PVV+VAVE KN  DLPKLVEGLKRL+KSDP V C  
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522

Query: 215 EESGEHIVVGAGEXILRSXXRXLR 286
            ESGEHIV GAGE  L    + L+
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQ 546



 Score = 89.4 bits (212), Expect = 3e-19
 Identities = 42/74 (56%), Positives = 51/74 (68%)
 Frame = +3

Query: 324 PVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVNPRDDFKTRARX 503
           PVVSYRE+V+E S    LSK PNKHNR+FM A+ M + L   I+ G VNPRDDFK RAR 
Sbjct: 559 PVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARI 618

Query: 504 LTKSTEYDVTEXRK 545
           +     +DVT+ RK
Sbjct: 619 MADEFGWDVTDARK 632



 Score = 26.2 bits (55), Expect = 3.2
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = +1

Query: 256 HLEIXLKDXEEDH 294
           HLEI LKD +EDH
Sbjct: 537 HLEICLKDLQEDH 549


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  105 bits (252), Expect = 4e-24
 Identities = 54/84 (64%), Positives = 61/84 (72%)
 Frame = +2

Query: 35  LGKTGTIXTXKNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 214
           L K+GT+ T + AHNMKVMK S  PVV+VAVE KN  DLPKLVEGLKRL+KSDP V C  
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522

Query: 215 EESGEHIVVGAGEXILRSXXRXLR 286
            ESGEHIV GAGE  L    + L+
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQ 546



 Score = 89.4 bits (212), Expect = 3e-19
 Identities = 42/74 (56%), Positives = 51/74 (68%)
 Frame = +3

Query: 324 PVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVNPRDDFKTRARX 503
           PVVSYRE+V+E S    LSK PNKHNR+FM A+ M + L   I+ G VNPRDDFK RAR 
Sbjct: 559 PVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARI 618

Query: 504 LTKSTEYDVTEXRK 545
           +     +DVT+ RK
Sbjct: 619 MADEFGWDVTDARK 632



 Score = 26.2 bits (55), Expect = 3.2
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = +1

Query: 256 HLEIXLKDXEEDH 294
           HLEI LKD +EDH
Sbjct: 537 HLEICLKDLQEDH 549


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 67.3 bits (157), Expect = 1e-12
 Identities = 33/82 (40%), Positives = 49/82 (59%)
 Frame = +2

Query: 41  KTGTIXTXKNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEE 220
           +T T+ +  N  N+  +     P+VRVA+EP  P ++ KLV GL  L ++DP VQ   EE
Sbjct: 540 RTATLCSSPNGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAVEE 599

Query: 221 SGEHIVVGAGEXILRSXXRXLR 286
           +GEH+++ AGE  L    + LR
Sbjct: 600 NGEHVIMCAGEIHLERCLKDLR 621


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 57.6 bits (133), Expect = 1e-09
 Identities = 28/59 (47%), Positives = 38/59 (64%)
 Frame = +2

Query: 110 VVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVGAGEXILRSXXRXLR 286
           V +VAVEP NP++LPKL++GL++  KS P+     EESGEH + G GE  +      LR
Sbjct: 600 VFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLR 658



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 25/77 (32%), Positives = 38/77 (49%)
 Frame = +3

Query: 312 RSDDPVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVNPRDDFKT 491
           R  DPV  + ET  + S   C S  PNK NR+ M  + +  G+  DI+ G+VN     K 
Sbjct: 667 RVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWPQKR 726

Query: 492 RARXLTKSTEYDVTEXR 542
            +    K+ ++D+   R
Sbjct: 727 ISEFFQKNYDWDLLASR 743


>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 813

 Score = 31.1 bits (67), Expect = 0.11
 Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
 Frame = +2

Query: 107 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVVGAG 250
           PV   ++EP +  D P L+E L  + + DP  +   + E+G+ ++ G G
Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMG 491


>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
           Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 241

 Score = 28.3 bits (60), Expect = 0.79
 Identities = 13/28 (46%), Positives = 19/28 (67%), Gaps = 2/28 (7%)
 Frame = +2

Query: 140 PADLPKLVEGLKRLAKSD--PMVQCINE 217
           P D+ KL EGL++L + D   +VQ +NE
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNE 199


>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1253

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = +3

Query: 411 MKAQXMPDGLPEDIDEGRVNPRDDFKT 491
           ++A+     LPE IDEG+++  DD KT
Sbjct: 185 VRAKLRDPTLPEKIDEGKLSDVDDGKT 211


>SPCC1259.06 |||transcription factor TFIID complex subunit 8
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 222

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 16/61 (26%), Positives = 26/61 (42%)
 Frame = +1

Query: 355 RNRTSYVSQSXPTSTTVYS*RLXPCLMVCQRTLMRVX*IPAMTSRLALGILRKVQNTMLP 534
           +NR +YV    P     ++    P   V   +  ++  +    SRLA   LRK+ N   P
Sbjct: 124 QNRPAYVVNHLPPFPASHTYMATPVYPVRPTSPKQIRELATQESRLAEHALRKILNVNQP 183

Query: 535 K 537
           +
Sbjct: 184 R 184


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,811,315
Number of Sequences: 5004
Number of extensions: 29394
Number of successful extensions: 91
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)