Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0520.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 93 8e-20
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 73 1e-13
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 71 4e-13
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 71 4e-13
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 67 6e-12
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 5e-06
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 34 0.054
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 34 0.054
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 30 1.2
At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) / beta-fruc... 29 2.0
At2g47500.1 68415.m05929 kinesin motor protein-related 28 3.6
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 28 4.7
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 28 4.7
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 27 6.2
At1g73920.2 68414.m08561 lipase family protein similar to lipase... 27 6.2
At1g73920.1 68414.m08560 lipase family protein similar to lipase... 27 6.2
At2g02470.1 68415.m00186 PHD finger family protein contains Pfam... 27 8.3
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 93.5 bits (222), Expect = 8e-20
Identities = 50/84 (59%), Positives = 58/84 (69%), Gaps = 2/84 (2%)
Frame = +2
Query: 41 KTGTIXTXK--NAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 214
K T+ K +AH ++ MK S PVVRVAV+ K +DLPKLVEGLKRLAKSDPMV C
Sbjct: 463 KNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCTM 522
Query: 215 EESGEHIVVGAGEXILRSXXRXLR 286
EESGEHIV GAGE L + L+
Sbjct: 523 EESGEHIVAGAGELHLEICLKDLQ 546
Score = 84.2 bits (199), Expect = 5e-17
Identities = 37/69 (53%), Positives = 51/69 (73%)
Frame = +3
Query: 321 DPVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVNPRDDFKTRAR 500
DPVVS+RETV + S + +SK PNKHNRL+M+A+ M +GL E ID+GR+ PRDD K R++
Sbjct: 559 DPVVSFRETVCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSK 618
Query: 501 XLTKSTEYD 527
L + +D
Sbjct: 619 ILAEEFGWD 627
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 72.9 bits (171), Expect = 1e-13
Identities = 33/83 (39%), Positives = 52/83 (62%)
Frame = +2
Query: 65 KNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVG 244
++ + + +K + PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH ++G
Sbjct: 572 EDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILG 631
Query: 245 AGEXILRSXXRXLRRTMXXXAIK 313
GE L S + LR +K
Sbjct: 632 TGELYLDSIIKDLRELYSEVQVK 654
Score = 54.0 bits (124), Expect = 6e-08
Identities = 26/55 (47%), Positives = 35/55 (63%)
Frame = +3
Query: 306 QSRSDDPVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVN 470
Q + DPVVS+ ETV E S C ++ PNK N+L M A+ + GL EDI+ G V+
Sbjct: 652 QVKVADPVVSFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVS 706
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 71.3 bits (167), Expect = 4e-13
Identities = 32/83 (38%), Positives = 52/83 (62%)
Frame = +2
Query: 65 KNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVG 244
++ + + ++ + PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH ++G
Sbjct: 586 EDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILG 645
Query: 245 AGEXILRSXXRXLRRTMXXXAIK 313
GE L S + LR +K
Sbjct: 646 TGELYLDSIMKDLRELYSEVEVK 668
Score = 52.8 bits (121), Expect = 1e-07
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 321 DPVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVN 470
DPVVS+ ETV E S C ++ PNK N++ M A+ + GL EDI+ G V+
Sbjct: 671 DPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVS 720
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 71.3 bits (167), Expect = 4e-13
Identities = 32/83 (38%), Positives = 52/83 (62%)
Frame = +2
Query: 65 KNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVG 244
++ + + ++ + PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH ++G
Sbjct: 586 EDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILG 645
Query: 245 AGEXILRSXXRXLRRTMXXXAIK 313
GE L S + LR +K
Sbjct: 646 TGELYLDSIMKDLRELYSEVEVK 668
Score = 52.8 bits (121), Expect = 1e-07
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 321 DPVVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDIDEGRVN 470
DPVVS+ ETV E S C ++ PNK N++ M A+ + GL EDI+ G V+
Sbjct: 671 DPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVS 720
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 67.3 bits (157), Expect = 6e-12
Identities = 30/90 (33%), Positives = 51/90 (56%)
Frame = +2
Query: 17 RNXRPXLGKTGTIXTXKNAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGLKRLAKSDP 196
R P + K+ T+ + +N + M+ P +RVA+EP +PAD+ L++GL+ L ++DP
Sbjct: 507 RGLGPYISKSATLSSTRNCWPLASMEFQVSPTLRVAIEPSDPADMSALMKGLRLLNRADP 566
Query: 197 MVQCINEESGEHIVVGAGEXILRSXXRXLR 286
V+ GEH++ AGE L + L+
Sbjct: 567 FVEITVSARGEHVLAAAGEVHLERCVKDLK 596
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 47.6 bits (108), Expect = 5e-06
Identities = 24/62 (38%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +2
Query: 107 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ-CINEESGEHIVVGAGEXILRSXXRXL 283
PV++VA+EPK AD+ K+ GL +LA+ DP +EE + ++ G GE L L
Sbjct: 493 PVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRL 552
Query: 284 RR 289
+R
Sbjct: 553 KR 554
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 34.3 bits (75), Expect = 0.054
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 107 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVVGAGEXILRSXXRXL 283
PV+ +AV+P + + + L R K DP + ++ ESG+ I+ G GE L +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 284 RR 289
RR
Sbjct: 521 RR 522
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 34.3 bits (75), Expect = 0.054
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 107 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVVGAGEXILRSXXRXL 283
PV+ +AV+P + + + L R K DP + ++ ESG+ I+ G GE L +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 284 RR 289
RR
Sbjct: 521 RR 522
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 29.9 bits (64), Expect = 1.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +3
Query: 357 ESDQLCLSKXPNKHNRLFMKAQXMPDGLPEDI 452
E + LCL PN+H + + A+ +P LPE +
Sbjct: 44 EKENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At3g13790.1 68416.m01742 beta-fructosidase (BFRUCT1) /
beta-fructofuranosidase / cell wall invertase identical
to beta-fructofuranosidase GI:402740 from [Arabidopsis
thaliana]
Length = 584
Score = 29.1 bits (62), Expect = 2.0
Identities = 17/77 (22%), Positives = 31/77 (40%), Gaps = 1/77 (1%)
Frame = +2
Query: 20 NXRPXLGKTGTIXTXKNAHNMKVMKXSXXPVVRV-AVEPKNPADLPKLVEGLKRLAKSDP 196
N +P + TG + N+ K P +R P NP P V G+ + DP
Sbjct: 134 NGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDP 193
Query: 197 MVQCINEESGEHIVVGA 247
+ ++ +++G+
Sbjct: 194 TTAWLGQDKKWRVIIGS 210
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 28.3 bits (60), Expect = 3.6
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 4/50 (8%)
Frame = +2
Query: 35 LGKTGTIXTXK----NAHNMKVMKXSXXPVVRVAVEPKNPADLPKLVEGL 172
L +T +I K N N S +VR + K P D+PKL+E L
Sbjct: 204 LSRTSSINNEKAPSENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.9 bits (59), Expect = 4.7
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +2
Query: 116 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVGAGE 253
+VA+ +P D+ K+ E L RLA D + +S E + +G +
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSK 173
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.9 bits (59), Expect = 4.7
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +2
Query: 116 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVVGAGE 253
+VA+ +P D+ K+ E L RLA D + +S E + +G +
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSK 173
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/45 (31%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Frame = +2
Query: 107 PVVRVAVEPKNPADLPKLVEGLKRLAK-SDPMVQCINEESGEHIV 238
P+++ V P N AD+ VE +K + S+ + +C+ EE E+++
Sbjct: 63 PIIKKVVVP-NRADIKTSVEEVKAIIDDSEALYECLIEEGEEYLL 106
>At1g73920.2 68414.m08561 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 635
Score = 27.5 bits (58), Expect = 6.2
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = -3
Query: 222 DSSLIHCTMGSDLARRLRPSTSXGRSAGFLGSTATRTTGXXLNFITFMLW 73
D+S + + G D L PS S S ST+ + +ITF+LW
Sbjct: 20 DTSSLEYSSGEDSDGPLPPSPSSQSSRLSWASTSASSENHWTEWITFILW 69
>At1g73920.1 68414.m08560 lipase family protein similar to lipase
GB:CAA74737 [SP|O46108] from [Drosophila melanogaster]
Length = 704
Score = 27.5 bits (58), Expect = 6.2
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = -3
Query: 222 DSSLIHCTMGSDLARRLRPSTSXGRSAGFLGSTATRTTGXXLNFITFMLW 73
D+S + + G D L PS S S ST+ + +ITF+LW
Sbjct: 89 DTSSLEYSSGEDSDGPLPPSPSSQSSRLSWASTSASSENHWTEWITFILW 138
>At2g02470.1 68415.m00186 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 256
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = +3
Query: 327 VVSYRETVAEESDQLCLSKXPNKHNRLFMKAQXMPDGLPE 446
+V + +T E + LCL PN+ + + + +P LPE
Sbjct: 34 MVKFYQTCDPEKENLCLYGLPNETWEVNLPVEEVPPELPE 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,748,514
Number of Sequences: 28952
Number of extensions: 160520
Number of successful extensions: 383
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 381
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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