SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0518.Seq
         (598 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   110   1e-25
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   110   1e-25
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    90   2e-19
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    83   2e-17
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    40   2e-04
SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom...    29   0.39 
SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|...    27   2.1  
SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces ...    26   3.6  
SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom...    25   8.4  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  110 bits (265), Expect = 1e-25
 Identities = 50/79 (63%), Positives = 59/79 (74%)
 Frame = -1

Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
           P + EPV+L EIQ  E A+GGI  VLN++RGHVF E Q  GTP++ +KA LP NESFGFT
Sbjct: 722 PIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFT 781

Query: 325 ADLRSNTGGQAFPQCVFDH 269
            +LR  T GQAFPQ VFDH
Sbjct: 782 GELRQATAGQAFPQLVFDH 800



 Score = 48.4 bits (110), Expect = 8e-07
 Identities = 23/40 (57%), Positives = 26/40 (65%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASPVSWSLYIFVKFSV 463
           DAIHRGGGQIIPT RR +YA  L ASP+       V+  V
Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735



 Score = 41.5 bits (93), Expect = 9e-05
 Identities = 17/28 (60%), Positives = 20/28 (71%)
 Frame = -3

Query: 254 GDPCEPQSKPXNVXQETRKRKGLKEGLP 171
           GDP +P SKP  +  E RKRKGLKE +P
Sbjct: 806 GDPLDPTSKPGQIVCEARKRKGLKENVP 833


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  110 bits (265), Expect = 1e-25
 Identities = 50/79 (63%), Positives = 59/79 (74%)
 Frame = -1

Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
           P + EPV+L EIQ  E A+GGI  VLN++RGHVF E Q  GTP++ +KA LP NESFGFT
Sbjct: 722 PIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFT 781

Query: 325 ADLRSNTGGQAFPQCVFDH 269
            +LR  T GQAFPQ VFDH
Sbjct: 782 GELRQATAGQAFPQLVFDH 800



 Score = 48.4 bits (110), Expect = 8e-07
 Identities = 23/40 (57%), Positives = 26/40 (65%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASPVSWSLYIFVKFSV 463
           DAIHRGGGQIIPT RR +YA  L ASP+       V+  V
Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735



 Score = 41.5 bits (93), Expect = 9e-05
 Identities = 17/28 (60%), Positives = 20/28 (71%)
 Frame = -3

Query: 254 GDPCEPQSKPXNVXQETRKRKGLKEGLP 171
           GDP +P SKP  +  E RKRKGLKE +P
Sbjct: 806 GDPLDPTSKPGQIVCEARKRKGLKENVP 833


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 983

 Score = 90.2 bits (214), Expect = 2e-19
 Identities = 44/94 (46%), Positives = 60/94 (63%), Gaps = 2/94 (2%)
 Frame = -1

Query: 529  VCMSA--NCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAX 356
            VC S+     PRLMEPVY+ E+  P  ++  I  +L RRRGHV ++    G+P+++V+A 
Sbjct: 828  VCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRAL 887

Query: 355  LPXNESFGFTADLRSNTGGQAFPQCVFDHXQVXP 254
            +P  +S GF  DLR +T GQA  Q VFDH QV P
Sbjct: 888  IPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVP 921



 Score = 41.5 bits (93), Expect = 9e-05
 Identities = 18/27 (66%), Positives = 21/27 (77%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502
           + I+RGGGQIIPT RR  Y+  LTASP
Sbjct: 812 EQIYRGGGQIIPTARRVCYSSFLTASP 838


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1000

 Score = 83.4 bits (197), Expect = 2e-17
 Identities = 36/85 (42%), Positives = 52/85 (61%)
 Frame = -1

Query: 505  PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
            PRLM  +Y C++Q     +G + GV+++RRG V +E    GTP FIVKA +P  ESFGF 
Sbjct: 860  PRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFA 919

Query: 325  ADLRSNTGGQAFPQCVFDHXQVXPE 251
             ++   T G A+PQ +F   ++  E
Sbjct: 920  VEILKRTSGAAYPQLIFHGFEMLDE 944


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 19/70 (27%), Positives = 37/70 (52%)
 Frame = -1

Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
           P ++EP+    I  P    GG+ G L++R+  + +         F ++A +P N  F ++
Sbjct: 672 PMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYS 729

Query: 325 ADLRSNTGGQ 296
           +D+R+ T G+
Sbjct: 730 SDIRALTKGK 739


>SPAC3C7.09 |set8||lysine methyltransferase Set8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 429

 Score = 29.5 bits (63), Expect = 0.39
 Identities = 13/38 (34%), Positives = 19/38 (50%)
 Frame = -2

Query: 330 LLPICVPTPADRPSRSAYSTXGRSXRRPVRTSEQAXQR 217
           +LP+ + TPA  P +  YS  G S   PV    +  Q+
Sbjct: 109 VLPLSINTPAQWPEKEVYSLQGTSIFNPVCVKRKILQQ 146


>SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 578

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 3/43 (6%)
 Frame = -1

Query: 559 PNHSNX*KMLVCMSANCQPRLMEPVYLCEIQC---PEVAVGGI 440
           P+H+N   + + +S N + RLMEP    EIQC   P+ A+G I
Sbjct: 469 PSHANEDMVRLRISRNGEERLMEP--KDEIQCLNLPKSALGTI 509


>SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 306

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = -2

Query: 534 CLYACLLTASPVSWSLYIF 478
           CL A  LT+SP  W+ Y F
Sbjct: 141 CLLAAALTSSPYKWAYYGF 159


>SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 350

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 13/41 (31%), Positives = 20/41 (48%), Gaps = 3/41 (7%)
 Frame = +1

Query: 28  SHLRVCFNMMRNM---CC*K*TCVKRFPYRYVFLICFNYVV 141
           SH  +C N +  +   C    TC+ R  YRY F+   + V+
Sbjct: 196 SHCHLCDNCVEYLDHHCIWLNTCIGRRNYRYYFIFLLSVVL 236


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,260,896
Number of Sequences: 5004
Number of extensions: 41621
Number of successful extensions: 88
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -