BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0518.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 110 1e-25 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 110 1e-25 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 90 2e-19 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 83 2e-17 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 40 2e-04 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 29 0.39 SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 27 2.1 SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces ... 26 3.6 SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom... 25 8.4 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 110 bits (265), Expect = 1e-25 Identities = 50/79 (63%), Positives = 59/79 (74%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 P + EPV+L EIQ E A+GGI VLN++RGHVF E Q GTP++ +KA LP NESFGFT Sbjct: 722 PIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFT 781 Query: 325 ADLRSNTGGQAFPQCVFDH 269 +LR T GQAFPQ VFDH Sbjct: 782 GELRQATAGQAFPQLVFDH 800 Score = 48.4 bits (110), Expect = 8e-07 Identities = 23/40 (57%), Positives = 26/40 (65%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASPVSWSLYIFVKFSV 463 DAIHRGGGQIIPT RR +YA L ASP+ V+ V Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 41.5 bits (93), Expect = 9e-05 Identities = 17/28 (60%), Positives = 20/28 (71%) Frame = -3 Query: 254 GDPCEPQSKPXNVXQETRKRKGLKEGLP 171 GDP +P SKP + E RKRKGLKE +P Sbjct: 806 GDPLDPTSKPGQIVCEARKRKGLKENVP 833 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 110 bits (265), Expect = 1e-25 Identities = 50/79 (63%), Positives = 59/79 (74%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 P + EPV+L EIQ E A+GGI VLN++RGHVF E Q GTP++ +KA LP NESFGFT Sbjct: 722 PIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFT 781 Query: 325 ADLRSNTGGQAFPQCVFDH 269 +LR T GQAFPQ VFDH Sbjct: 782 GELRQATAGQAFPQLVFDH 800 Score = 48.4 bits (110), Expect = 8e-07 Identities = 23/40 (57%), Positives = 26/40 (65%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASPVSWSLYIFVKFSV 463 DAIHRGGGQIIPT RR +YA L ASP+ V+ V Sbjct: 696 DAIHRGGGQIIPTARRVVYASTLLASPIIQEPVFLVEIQV 735 Score = 41.5 bits (93), Expect = 9e-05 Identities = 17/28 (60%), Positives = 20/28 (71%) Frame = -3 Query: 254 GDPCEPQSKPXNVXQETRKRKGLKEGLP 171 GDP +P SKP + E RKRKGLKE +P Sbjct: 806 GDPLDPTSKPGQIVCEARKRKGLKENVP 833 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 90.2 bits (214), Expect = 2e-19 Identities = 44/94 (46%), Positives = 60/94 (63%), Gaps = 2/94 (2%) Frame = -1 Query: 529 VCMSA--NCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAX 356 VC S+ PRLMEPVY+ E+ P ++ I +L RRRGHV ++ G+P+++V+A Sbjct: 828 VCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRAL 887 Query: 355 LPXNESFGFTADLRSNTGGQAFPQCVFDHXQVXP 254 +P +S GF DLR +T GQA Q VFDH QV P Sbjct: 888 IPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVP 921 Score = 41.5 bits (93), Expect = 9e-05 Identities = 18/27 (66%), Positives = 21/27 (77%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502 + I+RGGGQIIPT RR Y+ LTASP Sbjct: 812 EQIYRGGGQIIPTARRVCYSSFLTASP 838 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 83.4 bits (197), Expect = 2e-17 Identities = 36/85 (42%), Positives = 52/85 (61%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 PRLM +Y C++Q +G + GV+++RRG V +E GTP FIVKA +P ESFGF Sbjct: 860 PRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVKALIPVVESFGFA 919 Query: 325 ADLRSNTGGQAFPQCVFDHXQVXPE 251 ++ T G A+PQ +F ++ E Sbjct: 920 VEILKRTSGAAYPQLIFHGFEMLDE 944 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 40.3 bits (90), Expect = 2e-04 Identities = 19/70 (27%), Positives = 37/70 (52%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 P ++EP+ I P GG+ G L++R+ + + F ++A +P N F ++ Sbjct: 672 PMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYS 729 Query: 325 ADLRSNTGGQ 296 +D+R+ T G+ Sbjct: 730 SDIRALTKGK 739 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 29.5 bits (63), Expect = 0.39 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = -2 Query: 330 LLPICVPTPADRPSRSAYSTXGRSXRRPVRTSEQAXQR 217 +LP+ + TPA P + YS G S PV + Q+ Sbjct: 109 VLPLSINTPAQWPEKEVYSLQGTSIFNPVCVKRKILQQ 146 >SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 578 Score = 27.1 bits (57), Expect = 2.1 Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 3/43 (6%) Frame = -1 Query: 559 PNHSNX*KMLVCMSANCQPRLMEPVYLCEIQC---PEVAVGGI 440 P+H+N + + +S N + RLMEP EIQC P+ A+G I Sbjct: 469 PSHANEDMVRLRISRNGEERLMEP--KDEIQCLNLPKSALGTI 509 >SPCC31H12.02c |mug73||membrane transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 306 Score = 26.2 bits (55), Expect = 3.6 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -2 Query: 534 CLYACLLTASPVSWSLYIF 478 CL A LT+SP W+ Y F Sbjct: 141 CLLAAALTSSPYKWAYYGF 159 >SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 350 Score = 25.0 bits (52), Expect = 8.4 Identities = 13/41 (31%), Positives = 20/41 (48%), Gaps = 3/41 (7%) Frame = +1 Query: 28 SHLRVCFNMMRNM---CC*K*TCVKRFPYRYVFLICFNYVV 141 SH +C N + + C TC+ R YRY F+ + V+ Sbjct: 196 SHCHLCDNCVEYLDHHCIWLNTCIGRRNYRYYFIFLLSVVL 236 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,260,896 Number of Sequences: 5004 Number of extensions: 41621 Number of successful extensions: 88 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 80 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 87 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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