BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0518.Seq (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 121 3e-28 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 95 2e-20 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 95 2e-20 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 93 1e-19 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 68 4e-12 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 3e-07 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 3e-07 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 46 1e-05 At5g13650.2 68418.m01585 elongation factor family protein contai... 39 0.002 At5g13650.1 68418.m01584 elongation factor family protein contai... 39 0.002 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 30 1.3 At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1) ident... 27 7.2 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 121 bits (292), Expect = 3e-28 Identities = 53/93 (56%), Positives = 70/93 (75%) Frame = -1 Query: 538 KMLVCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKA 359 +++ +PRL+EPVY+ EIQ PE A+GGI VLN++RGHVFEE Q GTP++ +KA Sbjct: 712 RVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKA 771 Query: 358 XLPXNESFGFTADLRSNTGGQAFPQCVFDHXQV 260 LP ESFGF++ LR+ T GQAFPQCVFDH ++ Sbjct: 772 YLPVVESFGFSSQLRAATSGQAFPQCVFDHWEM 804 Score = 46.4 bits (105), Expect = 1e-05 Identities = 19/27 (70%), Positives = 22/27 (81%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502 DAIHRGGGQ+IPT RR +YA +TA P Sbjct: 697 DAIHRGGGQVIPTARRVIYASQITAKP 723 Score = 28.7 bits (61), Expect = 3.1 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = -3 Query: 251 DPCEPQSKPXNVXQETRKRKGLKEGL 174 DP EP ++ + + RKRKGLKE + Sbjct: 808 DPLEPGTQASVLVADIRKRKGLKEAM 833 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 95.5 bits (227), Expect = 2e-20 Identities = 49/84 (58%), Positives = 54/84 (64%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 PRLMEPVY EIQ P V I VL+RRRGHV + GTP +IVKA LP ESFGF Sbjct: 839 PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFE 898 Query: 325 ADLRSNTGGQAFPQCVFDHXQVXP 254 DLR +T GQAF VFDH + P Sbjct: 899 TDLRYHTQGQAFCLSVFDHWAIVP 922 Score = 36.3 bits (80), Expect = 0.016 Identities = 14/27 (51%), Positives = 19/27 (70%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502 + +HRG GQ+IPT RR Y+ L A+P Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATP 839 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 95.5 bits (227), Expect = 2e-20 Identities = 49/84 (58%), Positives = 54/84 (64%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 PRLMEPVY EIQ P V I VL+RRRGHV + GTP +IVKA LP ESFGF Sbjct: 839 PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFE 898 Query: 325 ADLRSNTGGQAFPQCVFDHXQVXP 254 DLR +T GQAF VFDH + P Sbjct: 899 TDLRYHTQGQAFCLSVFDHWAIVP 922 Score = 36.3 bits (80), Expect = 0.016 Identities = 14/27 (51%), Positives = 19/27 (70%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502 + +HRG GQ+IPT RR Y+ L A+P Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATP 839 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 93.1 bits (221), Expect = 1e-19 Identities = 48/84 (57%), Positives = 54/84 (64%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 PRLMEPVY EIQ P V I VL+RRRG+V + GTP +IVKA LP ESFGF Sbjct: 825 PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFE 884 Query: 325 ADLRSNTGGQAFPQCVFDHXQVXP 254 DLR +T GQAF VFDH + P Sbjct: 885 TDLRYHTQGQAFCLSVFDHWAIVP 908 Score = 36.3 bits (80), Expect = 0.016 Identities = 14/27 (51%), Positives = 19/27 (70%) Frame = -2 Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502 + +HRG GQ+IPT RR Y+ L A+P Sbjct: 799 EPLHRGSGQMIPTARRVAYSAFLMATP 825 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 68.1 bits (159), Expect = 4e-12 Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 2/94 (2%) Frame = -1 Query: 526 CMSANCQ--PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXL 353 C +A Q PR++E +Y CE+ +G + VL+RRR + +E G+ +F V A + Sbjct: 863 CRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYV 922 Query: 352 PXNESFGFTADLRSNTGGQAFPQCVFDHXQVXPE 251 P +ESFGF +LR T G A V H ++ E Sbjct: 923 PVSESFGFADELRKGTSGGASALMVLSHWEMLEE 956 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 52.0 bits (119), Expect = 3e-07 Identities = 31/99 (31%), Positives = 51/99 (51%), Gaps = 1/99 (1%) Frame = -1 Query: 529 VCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLP 350 +C +A +P ++EPV L E++ P G + G +N+R+G + Q ++ A +P Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704 Query: 349 XNESFGFTADLRSNTGGQA-FPQCVFDHXQVXPETRANL 236 N FG++ LRS T G+ F +H V E +A L Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 52.0 bits (119), Expect = 3e-07 Identities = 31/99 (31%), Positives = 51/99 (51%), Gaps = 1/99 (1%) Frame = -1 Query: 529 VCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLP 350 +C +A +P ++EPV L E++ P G + G +N+R+G + Q ++ A +P Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704 Query: 349 XNESFGFTADLRSNTGGQA-FPQCVFDHXQVXPETRANL 236 N FG++ LRS T G+ F +H V E +A L Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 46.4 bits (105), Expect = 1e-05 Identities = 24/71 (33%), Positives = 38/71 (53%) Frame = -1 Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326 PR++EP+ E+ PE +G + G LN RRG + G + +V + +P E F + Sbjct: 685 PRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYV 743 Query: 325 ADLRSNTGGQA 293 + LR T G+A Sbjct: 744 STLRGMTKGRA 754 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 39.1 bits (87), Expect = 0.002 Identities = 19/77 (24%), Positives = 36/77 (46%) Frame = -1 Query: 502 RLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFTA 323 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532 Query: 322 DLRSNTGGQAFPQCVFD 272 + + + G A VFD Sbjct: 533 AILTASRGTAILNTVFD 549 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 39.1 bits (87), Expect = 0.002 Identities = 19/77 (24%), Positives = 36/77 (46%) Frame = -1 Query: 502 RLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFTA 323 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531 Query: 322 DLRSNTGGQAFPQCVFD 272 + + + G A VFD Sbjct: 532 AILTASRGTAILNTVFD 548 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 29.9 bits (64), Expect = 1.3 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = -1 Query: 493 EPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNE-SFGFTADL 317 EP + I P VG + + + RRG E + + +F+ K LP E F +L Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524 Query: 316 RSNTGGQA 293 +S T G A Sbjct: 525 KSITSGYA 532 >At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1) identical to pseudo-response regulator 1 GI:7576354 from [Arabidopsis thaliana]; timing of CAB expression 1 protein (TOC1) GI:9247019; contains Pfam profile PF00072: Response regulator receiver domain; identical to cDNA ABI3-interacting protein 1 (aip1 gene) GI:6996312 Length = 618 Score = 27.5 bits (58), Expect = 7.2 Identities = 14/49 (28%), Positives = 25/49 (51%) Frame = +2 Query: 146 ICPNIELSLGDLPSILSSFSFPVQRCXACSEVRTGLRXDLPMVEYALRE 292 +C N SLG++ ++LS S+ V + +V L + P ++ L E Sbjct: 23 LCDNDSTSLGEVFTLLSECSYQVTAVKSARQVIDALNAEGPDIDIILAE 71 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,004,796 Number of Sequences: 28952 Number of extensions: 225415 Number of successful extensions: 437 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 427 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 435 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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