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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0518.Seq
         (598 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p...   121   3e-28
At1g06220.2 68414.m00656 elongation factor Tu family protein sim...    95   2e-20
At1g06220.1 68414.m00655 elongation factor Tu family protein sim...    95   2e-20
At5g25230.1 68418.m02991 elongation factor Tu family protein tra...    93   1e-19
At3g22980.1 68416.m02898 elongation factor Tu family protein sim...    68   4e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati...    52   3e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati...    52   3e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim...    46   1e-05
At5g13650.2 68418.m01585 elongation factor family protein contai...    39   0.002
At5g13650.1 68418.m01584 elongation factor family protein contai...    39   0.002
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-...    30   1.3  
At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1) ident...    27   7.2  

>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
           putative similar to ELONGATION FACTOR 2 GB:O14460 from
           [Schizosaccharomyces pombe]
          Length = 843

 Score =  121 bits (292), Expect = 3e-28
 Identities = 53/93 (56%), Positives = 70/93 (75%)
 Frame = -1

Query: 538 KMLVCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKA 359
           +++       +PRL+EPVY+ EIQ PE A+GGI  VLN++RGHVFEE Q  GTP++ +KA
Sbjct: 712 RVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKA 771

Query: 358 XLPXNESFGFTADLRSNTGGQAFPQCVFDHXQV 260
            LP  ESFGF++ LR+ T GQAFPQCVFDH ++
Sbjct: 772 YLPVVESFGFSSQLRAATSGQAFPQCVFDHWEM 804



 Score = 46.4 bits (105), Expect = 1e-05
 Identities = 19/27 (70%), Positives = 22/27 (81%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502
           DAIHRGGGQ+IPT RR +YA  +TA P
Sbjct: 697 DAIHRGGGQVIPTARRVIYASQITAKP 723



 Score = 28.7 bits (61), Expect = 3.1
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = -3

Query: 251 DPCEPQSKPXNVXQETRKRKGLKEGL 174
           DP EP ++   +  + RKRKGLKE +
Sbjct: 808 DPLEPGTQASVLVADIRKRKGLKEAM 833


>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 95.5 bits (227), Expect = 2e-20
 Identities = 49/84 (58%), Positives = 54/84 (64%)
 Frame = -1

Query: 505  PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
            PRLMEPVY  EIQ P   V  I  VL+RRRGHV  +    GTP +IVKA LP  ESFGF 
Sbjct: 839  PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFE 898

Query: 325  ADLRSNTGGQAFPQCVFDHXQVXP 254
             DLR +T GQAF   VFDH  + P
Sbjct: 899  TDLRYHTQGQAFCLSVFDHWAIVP 922



 Score = 36.3 bits (80), Expect = 0.016
 Identities = 14/27 (51%), Positives = 19/27 (70%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502
           + +HRG GQ+IPT RR  Y+  L A+P
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATP 839


>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
            to Cryptosporidium parvum elongation factor-2 GB:U21667
            GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 95.5 bits (227), Expect = 2e-20
 Identities = 49/84 (58%), Positives = 54/84 (64%)
 Frame = -1

Query: 505  PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
            PRLMEPVY  EIQ P   V  I  VL+RRRGHV  +    GTP +IVKA LP  ESFGF 
Sbjct: 839  PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFE 898

Query: 325  ADLRSNTGGQAFPQCVFDHXQVXP 254
             DLR +T GQAF   VFDH  + P
Sbjct: 899  TDLRYHTQGQAFCLSVFDHWAIVP 922



 Score = 36.3 bits (80), Expect = 0.016
 Identities = 14/27 (51%), Positives = 19/27 (70%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502
           + +HRG GQ+IPT RR  Y+  L A+P
Sbjct: 813 EPLHRGSGQMIPTARRVAYSAFLMATP 839


>At5g25230.1 68418.m02991 elongation factor Tu family protein
            translation Elongation Factor 2, Schizosaccharomyces
            pombe, PIR:T39902
          Length = 973

 Score = 93.1 bits (221), Expect = 1e-19
 Identities = 48/84 (57%), Positives = 54/84 (64%)
 Frame = -1

Query: 505  PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
            PRLMEPVY  EIQ P   V  I  VL+RRRG+V  +    GTP +IVKA LP  ESFGF 
Sbjct: 825  PRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFE 884

Query: 325  ADLRSNTGGQAFPQCVFDHXQVXP 254
             DLR +T GQAF   VFDH  + P
Sbjct: 885  TDLRYHTQGQAFCLSVFDHWAIVP 908



 Score = 36.3 bits (80), Expect = 0.016
 Identities = 14/27 (51%), Positives = 19/27 (70%)
 Frame = -2

Query: 582 DAIHRGGGQIIPTXRRCLYACLLTASP 502
           + +HRG GQ+IPT RR  Y+  L A+P
Sbjct: 799 EPLHRGSGQMIPTARRVAYSAFLMATP 825


>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
            to eukaryotic translation elongation factor 2
            GB:NP_001952 [Homo sapiens]
          Length = 1015

 Score = 68.1 bits (159), Expect = 4e-12
 Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 2/94 (2%)
 Frame = -1

Query: 526  CMSANCQ--PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXL 353
            C +A  Q  PR++E +Y CE+      +G +  VL+RRR  + +E    G+ +F V A +
Sbjct: 863  CRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYV 922

Query: 352  PXNESFGFTADLRSNTGGQAFPQCVFDHXQVXPE 251
            P +ESFGF  +LR  T G A    V  H ++  E
Sbjct: 923  PVSESFGFADELRKGTSGGASALMVLSHWEMLEE 956


>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
           similar to SP|P25039 Elongation factor G 1,
           mitochondrial precursor (mEF-G-1) {Saccharomyces
           cerevisiae}; contains Pfam profiles PF00009: Elongation
           factor Tu GTP binding domain, PF03764: Elongation factor
           G domain IV, PF00679: Elongation factor G C-terminus
          Length = 754

 Score = 52.0 bits (119), Expect = 3e-07
 Identities = 31/99 (31%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
 Frame = -1

Query: 529 VCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLP 350
           +C +A  +P ++EPV L E++ P    G + G +N+R+G +    Q       ++ A +P
Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704

Query: 349 XNESFGFTADLRSNTGGQA-FPQCVFDHXQVXPETRANL 236
            N  FG++  LRS T G+  F     +H  V  E +A L
Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743


>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
           similar to mitochondrial elongation factor GI:3917 from
           [Saccharomyces cerevisiae]
          Length = 754

 Score = 52.0 bits (119), Expect = 3e-07
 Identities = 31/99 (31%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
 Frame = -1

Query: 529 VCMSANCQPRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLP 350
           +C +A  +P ++EPV L E++ P    G + G +N+R+G +    Q       ++ A +P
Sbjct: 648 LCYTA-ARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVP 704

Query: 349 XNESFGFTADLRSNTGGQA-FPQCVFDHXQVXPETRANL 236
            N  FG++  LRS T G+  F     +H  V  E +A L
Sbjct: 705 LNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQL 743


>At1g62750.1 68414.m07082 elongation factor Tu family protein
           similar to elongation factor G SP:P34811 [Glycine max
           (Soybean)]
          Length = 783

 Score = 46.4 bits (105), Expect = 1e-05
 Identities = 24/71 (33%), Positives = 38/71 (53%)
 Frame = -1

Query: 505 PRLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFT 326
           PR++EP+   E+  PE  +G + G LN RRG +       G  + +V + +P  E F + 
Sbjct: 685 PRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYV 743

Query: 325 ADLRSNTGGQA 293
           + LR  T G+A
Sbjct: 744 STLRGMTKGRA 754


>At5g13650.2 68418.m01585 elongation factor family protein contains
           Pfam profiles: PF00009 elongation factor Tu GTP binding
           domain,PF00679 elongation factor G C-terminus,  PF03144
           elongation factor Tu domain 2
          Length = 676

 Score = 39.1 bits (87), Expect = 0.002
 Identities = 19/77 (24%), Positives = 36/77 (46%)
 Frame = -1

Query: 502 RLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFTA 323
           +L+EP  +  ++ PE  +G +  +L +RRG +F+   V       ++  +P     G   
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532

Query: 322 DLRSNTGGQAFPQCVFD 272
            + + + G A    VFD
Sbjct: 533 AILTASRGTAILNTVFD 549


>At5g13650.1 68418.m01584 elongation factor family protein contains
           Pfam profiles: PF00009 elongation factor Tu GTP binding
           domain,PF00679 elongation factor G C-terminus,  PF03144
           elongation factor Tu domain 2
          Length = 675

 Score = 39.1 bits (87), Expect = 0.002
 Identities = 19/77 (24%), Positives = 36/77 (46%)
 Frame = -1

Query: 502 RLMEPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNESFGFTA 323
           +L+EP  +  ++ PE  +G +  +L +RRG +F+   V       ++  +P     G   
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531

Query: 322 DLRSNTGGQAFPQCVFD 272
            + + + G A    VFD
Sbjct: 532 AILTASRGTAILNTVFD 548


>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
           GTP-binding protein GUF1 - Saccharomyces cerevisiae,
           PIR:S50374
          Length = 661

 Score = 29.9 bits (64), Expect = 1.3
 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 1/68 (1%)
 Frame = -1

Query: 493 EPVYLCEIQCPEVAVGGIXGVLNRRRGHVFEESQVAGTPMFIVKAXLPXNE-SFGFTADL 317
           EP  +  I  P   VG +  + + RRG   E + +    +F+ K  LP  E    F  +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524

Query: 316 RSNTGGQA 293
           +S T G A
Sbjct: 525 KSITSGYA 532


>At5g61380.1 68418.m07701 ABI3-interacting protein 1 (AIP1)
           identical to pseudo-response regulator 1 GI:7576354 from
           [Arabidopsis thaliana]; timing of CAB expression 1
           protein (TOC1) GI:9247019; contains Pfam profile
           PF00072:  Response regulator receiver domain; identical
           to cDNA ABI3-interacting protein 1 (aip1 gene)
           GI:6996312
          Length = 618

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 14/49 (28%), Positives = 25/49 (51%)
 Frame = +2

Query: 146 ICPNIELSLGDLPSILSSFSFPVQRCXACSEVRTGLRXDLPMVEYALRE 292
           +C N   SLG++ ++LS  S+ V    +  +V   L  + P ++  L E
Sbjct: 23  LCDNDSTSLGEVFTLLSECSYQVTAVKSARQVIDALNAEGPDIDIILAE 71


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,004,796
Number of Sequences: 28952
Number of extensions: 225415
Number of successful extensions: 437
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 435
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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