BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0515.Seq
(449 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.19
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 24 9.4
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 24 9.4
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.9 bits (64), Expect = 0.19
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +2
Query: 119 PAVAALMDTVPTVVMEVYHPPAVPVTLIQTQTARDP 226
PAV+A + T P VV V HP P I T +DP
Sbjct: 1293 PAVSASISTPPAVVPTVQHPQ--PTKQIPTAAVKDP 1326
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 24.2 bits (50), Expect = 9.4
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +2
Query: 248 LMVQFQQS*WKCIIHRLFQXHXIQTQTXRDP 340
L Q QS WK I +++F I T + R+P
Sbjct: 253 LSTQDLQSGWKFIDNQMFLSTQINTSSLREP 283
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 24.2 bits (50), Expect = 9.4
Identities = 19/58 (32%), Positives = 23/58 (39%)
Frame = +2
Query: 62 PPAVPAQLMQAQTA*DLVHPAVAALMDTVPTVVMEVYHPPAVPVTLIQTQTARDPVHP 235
PP P M A PA+A+ P V E YHPP T + + P HP
Sbjct: 908 PPPPPTASMTASA------PAIAS---PPPPKVGETYHPPTASGT--RVPPVQQPSHP 954
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.131 0.381
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,324,204
Number of Sequences: 5004
Number of extensions: 20930
Number of successful extensions: 45
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 166231220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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