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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0515.Seq
         (449 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    30   0.19 
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po...    24   9.4  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    24   9.4  

>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 29.9 bits (64), Expect = 0.19
 Identities = 16/36 (44%), Positives = 19/36 (52%)
 Frame = +2

Query: 119  PAVAALMDTVPTVVMEVYHPPAVPVTLIQTQTARDP 226
            PAV+A + T P VV  V HP   P   I T   +DP
Sbjct: 1293 PAVSASISTPPAVVPTVQHPQ--PTKQIPTAAVKDP 1326


>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 361

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +2

Query: 248 LMVQFQQS*WKCIIHRLFQXHXIQTQTXRDP 340
           L  Q  QS WK I +++F    I T + R+P
Sbjct: 253 LSTQDLQSGWKFIDNQMFLSTQINTSSLREP 283


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 19/58 (32%), Positives = 23/58 (39%)
 Frame = +2

Query: 62   PPAVPAQLMQAQTA*DLVHPAVAALMDTVPTVVMEVYHPPAVPVTLIQTQTARDPVHP 235
            PP  P   M A        PA+A+     P  V E YHPP    T  +    + P HP
Sbjct: 908  PPPPPTASMTASA------PAIAS---PPPPKVGETYHPPTASGT--RVPPVQQPSHP 954


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.131    0.381 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,324,204
Number of Sequences: 5004
Number of extensions: 20930
Number of successful extensions: 45
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 166231220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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