Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0499.Seq
(499 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q96A72 Cluster: Protein mago nashi homolog 2; n=89; Euk... 115 5e-25
UniRef50_Q0ZCF1 Cluster: Mago nashi-like protein; n=2; Eukaryota... 105 7e-22
UniRef50_Q7RIE8 Cluster: Mago nashi protein homolog; n=4; Plasmo... 95 6e-19
UniRef50_Q55E21 Cluster: Putative uncharacterized protein; n=1; ... 84 1e-15
UniRef50_A7ATQ3 Cluster: Mago nashi protein, putative; n=1; Babe... 81 1e-14
UniRef50_Q0TZR8 Cluster: Predicted protein; n=5; Ascomycota|Rep:... 79 5e-14
UniRef50_Q4Q6T2 Cluster: Mago nashi-like protein,putative; n=4; ... 77 3e-13
UniRef50_Q55TE5 Cluster: Putative uncharacterized protein; n=1; ... 53 2e-12
UniRef50_A7QP77 Cluster: Chromosome chr1 scaffold_136, whole gen... 69 6e-11
UniRef50_A6NEC0 Cluster: Uncharacterized protein ENSP00000371305... 64 2e-09
UniRef50_UPI000049841C Cluster: mago nashi protein; n=1; Entamoe... 51 1e-05
UniRef50_Q0D192 Cluster: Mago nashi protein; n=1; Aspergillus te... 47 2e-04
UniRef50_Q3LW06 Cluster: Mago; n=1; Bigelowiella natans|Rep: Mag... 47 3e-04
UniRef50_Q4MP98 Cluster: Methyltransferase; n=13; Firmicutes|Rep... 33 2.7
UniRef50_Q4YEN5 Cluster: Putative uncharacterized protein; n=1; ... 32 8.2
UniRef50_Q4WAY3 Cluster: Polyketide synthase, putative; n=1; Asp... 32 8.2
>UniRef50_Q96A72 Cluster: Protein mago nashi homolog 2; n=89;
Eukaryota|Rep: Protein mago nashi homolog 2 - Homo
sapiens (Human)
Length = 148
Score = 115 bits (277), Expect = 5e-25
Identities = 55/70 (78%), Positives = 57/70 (81%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
GKLRYANNSNYKND MIRKEAYVH VM+ELKRII DSEI E LWP PDRVG ELE
Sbjct: 32 GKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELE 91
Query: 435 IVIGEEHIXF 464
IVIG+EHI F
Sbjct: 92 IVIGDEHISF 101
Score = 63.7 bits (148), Expect = 2e-09
Identities = 24/30 (80%), Positives = 29/30 (96%)
Frame = +1
Query: 169 MSTDFYIRYYVGHXGKFGHEFLEFEFRPEG 258
+++DFY+RYYVGH GKFGHEFLEFEFRP+G
Sbjct: 3 VASDFYLRYYVGHKGKFGHEFLEFEFRPDG 32
>UniRef50_Q0ZCF1 Cluster: Mago nashi-like protein; n=2;
Eukaryota|Rep: Mago nashi-like protein - Populus
trichocarpa (Western balsam poplar) (Populus
balsamiferasubsp. trichocarpa)
Length = 256
Score = 105 bits (251), Expect = 7e-22
Identities = 48/70 (68%), Positives = 55/70 (78%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
GKLRYANNSNYKNDTMIRKE ++ P V+ E +RII +SEIM E WP+PDRVG ELE
Sbjct: 36 GKLRYANNSNYKNDTMIRKEVFLTPAVLKECRRIISESEIMKEDDNNWPEPDRVGKQELE 95
Query: 435 IVIGEEHIXF 464
IV+G EHI F
Sbjct: 96 IVMGNEHISF 105
Score = 60.9 bits (141), Expect = 2e-08
Identities = 23/27 (85%), Positives = 26/27 (96%)
Frame = +1
Query: 178 DFYIRYYVGHXGKFGHEFLEFEFRPEG 258
+FY+RYYVGH GKFGHEFLEFEFRP+G
Sbjct: 10 EFYLRYYVGHRGKFGHEFLEFEFRPDG 36
>UniRef50_Q7RIE8 Cluster: Mago nashi protein homolog; n=4;
Plasmodium (Vinckeia)|Rep: Mago nashi protein homolog -
Plasmodium yoelii yoelii
Length = 187
Score = 95.5 bits (227), Expect = 6e-19
Identities = 49/99 (49%), Positives = 65/99 (65%), Gaps = 3/99 (3%)
Frame = +3
Query: 168 NVYGFLHTILCWTX--GKVWPRISRI*IPA*GKLRYANNSNYKNDTMIRKEAYVHPCVMD 341
N + F + C GK + + G+LRYANNSNYKND +IRKEAYV V++
Sbjct: 40 NFFNFFNFFFCSVGHKGKFGHEFLEFELNSKGRLRYANNSNYKNDKIIRKEAYVSKSVLN 99
Query: 342 ELKRIIIDSEIMHEXXRLWPQPDRVGWXELEIVI-GEEH 455
ELKRII +SEI E +LWP+PD+VG ELEI++ G+E+
Sbjct: 100 ELKRIIEESEICKESDKLWPEPDKVGKQELEIILDGKEY 138
>UniRef50_Q55E21 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 172
Score = 84.2 bits (199), Expect = 1e-15
Identities = 39/69 (56%), Positives = 51/69 (73%)
Frame = +3
Query: 258 KLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELEI 437
KLRYANNS+YK++ +IRKE V V+ E+K++I+DS IM E + WP+PD VG ELEI
Sbjct: 82 KLRYANNSHYKSENLIRKEVCVSDGVISEIKKVILDSGIMLEDDKNWPEPDVVGKQELEI 141
Query: 438 VIGEEHIXF 464
V +EHI F
Sbjct: 142 VFKDEHISF 150
Score = 59.7 bits (138), Expect = 4e-08
Identities = 28/48 (58%), Positives = 34/48 (70%), Gaps = 1/48 (2%)
Frame = +1
Query: 121 AAKVEVLEDKXKTLC-KMSTDFYIRYYVGHXGKFGHEFLEFEFRPEGN 261
+++ + ED KT K DFY+RYYVGH GK+GHEFLEFEFR GN
Sbjct: 35 SSETQTTEDILKTETQKQKDDFYLRYYVGHKGKYGHEFLEFEFR-SGN 81
>UniRef50_A7ATQ3 Cluster: Mago nashi protein, putative; n=1; Babesia
bovis|Rep: Mago nashi protein, putative - Babesia bovis
Length = 218
Score = 81.0 bits (191), Expect = 1e-14
Identities = 38/70 (54%), Positives = 49/70 (70%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
GKLRY NNSNY+ D+ I+KEA+V V+ EL+RII +SEI E WP PDR+G ELE
Sbjct: 31 GKLRYTNNSNYRKDSKIKKEAHVTRAVVAELRRIIEESEITSEDHSDWPIPDRIGRQELE 90
Query: 435 IVIGEEHIXF 464
+ +G +H F
Sbjct: 91 LRLGGKHYTF 100
Score = 50.8 bits (116), Expect = 2e-05
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFLEFEFRPEG 258
F++RYYVGH GKFGHEFLEFE +G
Sbjct: 6 FFLRYYVGHEGKFGHEFLEFELNDDG 31
>UniRef50_Q0TZR8 Cluster: Predicted protein; n=5; Ascomycota|Rep:
Predicted protein - Phaeosphaeria nodorum (Septoria
nodorum)
Length = 212
Score = 79.0 bits (186), Expect = 5e-14
Identities = 36/67 (53%), Positives = 48/67 (71%)
Frame = +3
Query: 264 RYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELEIVI 443
RYANNSNY+ND++IRKE V ++ E+KRI+ +SEI+ E WPQ ++ G ELEI +
Sbjct: 40 RYANNSNYRNDSLIRKEMCVSDALVAEIKRIVKESEILKEDDTKWPQKNKDGRQELEIRL 99
Query: 444 GEEHIXF 464
G EHI F
Sbjct: 100 GSEHISF 106
Score = 50.0 bits (114), Expect = 3e-05
Identities = 19/27 (70%), Positives = 23/27 (85%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFLEFEFRPEGN 261
FY+RYY GH G+FGHEFLEF+FR G+
Sbjct: 7 FYLRYYSGHQGRFGHEFLEFDFRVLGD 33
>UniRef50_Q4Q6T2 Cluster: Mago nashi-like protein,putative; n=4;
Trypanosomatidae|Rep: Mago nashi-like protein,putative -
Leishmania major
Length = 162
Score = 76.6 bits (180), Expect = 3e-13
Identities = 32/68 (47%), Positives = 45/68 (66%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
G L+Y NNSNY+ND +I+K+A V P V++E+K++I+ + WP PDR G ELE
Sbjct: 46 GSLKYVNNSNYRNDFIIKKQARVSPAVLEEVKKLIVQYSVCESDDERWPAPDRNGRQELE 105
Query: 435 IVIGEEHI 458
I +G HI
Sbjct: 106 IHLGNTHI 113
Score = 42.7 bits (96), Expect = 0.004
Identities = 15/27 (55%), Positives = 21/27 (77%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFLEFEFRPEGN 261
+Y+RYY GH G++G+EFLEFE G+
Sbjct: 21 YYVRYYSGHTGRYGNEFLEFEISETGS 47
>UniRef50_Q55TE5 Cluster: Putative uncharacterized protein; n=1;
Filobasidiella neoformans|Rep: Putative uncharacterized
protein - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 194
Score = 53.2 bits (122), Expect(2) = 2e-12
Identities = 26/54 (48%), Positives = 35/54 (64%)
Frame = +3
Query: 303 IRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELEIVIGEEHIXF 464
I + +V P V+DELKRI+ +SEI E WP+ + VG ELE+ I +EHI F
Sbjct: 93 IVEAVFVSPAVVDELKRIVRESEITKEDDVAWPRKNVVGKQELEVRIDKEHISF 146
Score = 44.0 bits (99), Expect = 0.002
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFLEFEF 246
FY+RYY GH G GHEFLEFE+
Sbjct: 11 FYLRYYTGHQGAHGHEFLEFEY 32
Score = 41.1 bits (92), Expect(2) = 2e-12
Identities = 15/21 (71%), Positives = 21/21 (100%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEA 317
G++RYANNSNY+ND++IRKE+
Sbjct: 35 GRIRYANNSNYRNDSLIRKES 55
>UniRef50_A7QP77 Cluster: Chromosome chr1 scaffold_136, whole genome
shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
chr1 scaffold_136, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 213
Score = 68.9 bits (161), Expect = 6e-11
Identities = 31/43 (72%), Positives = 36/43 (83%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHE 383
GKLRYANNSNYKNDTMIRKE +++P V+ +RII DSEIM E
Sbjct: 31 GKLRYANNSNYKNDTMIRKEVFLNPAVLRGCRRIIADSEIMKE 73
Score = 51.2 bits (117), Expect = 1e-05
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +1
Query: 184 YIRYYVGHXGKFGHEFLEFEFRPEG 258
Y+RYYVGH KFGHEFLEFE RP+G
Sbjct: 7 YLRYYVGHERKFGHEFLEFESRPDG 31
>UniRef50_A6NEC0 Cluster: Uncharacterized protein ENSP00000371305;
n=3; Eutheria|Rep: Uncharacterized protein
ENSP00000371305 - Homo sapiens (Human)
Length = 111
Score = 63.7 bits (148), Expect = 2e-09
Identities = 24/30 (80%), Positives = 29/30 (96%)
Frame = +1
Query: 169 MSTDFYIRYYVGHXGKFGHEFLEFEFRPEG 258
+++DFY+RYYVGH GKFGHEFLEFEFRP+G
Sbjct: 3 VASDFYLRYYVGHKGKFGHEFLEFEFRPDG 32
Score = 44.8 bits (101), Expect(2) = 9e-06
Identities = 19/20 (95%), Positives = 19/20 (95%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKE 314
GKLRYANNSNYKND MIRKE
Sbjct: 32 GKLRYANNSNYKNDVMIRKE 51
Score = 26.6 bits (56), Expect(2) = 9e-06
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +3
Query: 426 ELEIVIGEEHIXF 464
ELEIVIG+EHI F
Sbjct: 52 ELEIVIGDEHISF 64
>UniRef50_UPI000049841C Cluster: mago nashi protein; n=1; Entamoeba
histolytica HM-1:IMSS|Rep: mago nashi protein -
Entamoeba histolytica HM-1:IMSS
Length = 154
Score = 51.2 bits (117), Expect = 1e-05
Identities = 26/70 (37%), Positives = 39/70 (55%)
Frame = +3
Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
G+L+Y N S Y+N+T I K+ ++ VM E++ II S I+ + WP ELE
Sbjct: 38 GELKYCNQSRYRNETEIIKKLHLTRVVMKEIQDIISQSHILIQTDAHWPNKINGSRQELE 97
Query: 435 IVIGEEHIXF 464
I + + HI F
Sbjct: 98 IEMNDIHIFF 107
Score = 32.3 bits (70), Expect = 6.2
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFLEFEFRPEG 258
FY+RYY G+ ++G E+++FE G
Sbjct: 13 FYLRYYNGNHNQYGKEYIKFEIFDNG 38
>UniRef50_Q0D192 Cluster: Mago nashi protein; n=1; Aspergillus
terreus NIH2624|Rep: Mago nashi protein - Aspergillus
terreus (strain NIH 2624)
Length = 157
Score = 47.2 bits (107), Expect = 2e-04
Identities = 22/41 (53%), Positives = 28/41 (68%)
Frame = +3
Query: 342 ELKRIIIDSEIMHEXXRLWPQPDRVGWXELEIVIGEEHIXF 464
++KRII +SEIM E WPQ ++ G ELEI +G EHI F
Sbjct: 86 QIKRIIKESEIMKEDDSKWPQKNKDGRQELEIRLGNEHISF 126
Score = 39.5 bits (88), Expect = 0.041
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +1
Query: 181 FYIRYYVGHXGKFGHEFL 234
FY+RYY GH G+FGHEFL
Sbjct: 8 FYLRYYSGHSGRFGHEFL 25
>UniRef50_Q3LW06 Cluster: Mago; n=1; Bigelowiella natans|Rep: Mago -
Bigelowiella natans (Pedinomonas minutissima)
(Chlorarachnion sp.(strain CCMP 621))
Length = 148
Score = 46.8 bits (106), Expect = 3e-04
Identities = 26/85 (30%), Positives = 43/85 (50%)
Frame = +3
Query: 210 GKVWPRISRI*IPA*GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXX 389
G V I I + + G++ Y N S+ K+ + K+ +M+ + III EI+ E
Sbjct: 15 GSVSKEIEEIELNSEGEINYFNYSSGKSSNLNLKKIKTCSLLMNIISHIIIYHEILRETD 74
Query: 390 RLWPQPDRVGWXELEIVIGEEHIXF 464
+ W + D+ G+ ELEI+ H F
Sbjct: 75 KSWIRNDKTGFQELEILYRNRHFSF 99
>UniRef50_Q4MP98 Cluster: Methyltransferase; n=13; Firmicutes|Rep:
Methyltransferase - Bacillus cereus G9241
Length = 254
Score = 33.5 bits (73), Expect = 2.7
Identities = 23/73 (31%), Positives = 36/73 (49%)
Frame = -2
Query: 273 WHNAVSLRPEFKFEKFVAKLSXVSNIISYVKIRRHFAQSFXFILENFHFSGSTDTLKENY 94
W N V+L K+EK+ +K V + +R + + F +LE+ FS T NY
Sbjct: 177 WLNQVTLS-YLKYEKW-SKGQLVQTELQRFAVRWYGIEEFKLLLESIGFSNIT--CSANY 232
Query: 93 SMMRKPSNANYQL 55
++PSNAN +
Sbjct: 233 VYEKEPSNANQMI 245
>UniRef50_Q4YEN5 Cluster: Putative uncharacterized protein; n=1;
Plasmodium berghei|Rep: Putative uncharacterized protein
- Plasmodium berghei
Length = 151
Score = 31.9 bits (69), Expect = 8.2
Identities = 19/80 (23%), Positives = 39/80 (48%), Gaps = 2/80 (2%)
Frame = -2
Query: 354 FFLTRPLHKGEHRLLYVSLY-RFYS*NYWHNAVSLRPEFKFEKFVA-KLSXVSNIISYVK 181
+F+ +++ Y+++Y + Y Y+H ++L + F ++ + II YVK
Sbjct: 66 YFVRLKKKNNNNKIAYINMYFQAYHKYYYHIVINLIHFYSLLLFFFFSINEIQLIIKYVK 125
Query: 180 IRRHFAQSFXFILENFHFSG 121
HF++ +EN +F G
Sbjct: 126 PNIHFSKVERTYMENCNFHG 145
>UniRef50_Q4WAY3 Cluster: Polyketide synthase, putative; n=1;
Aspergillus fumigatus|Rep: Polyketide synthase, putative
- Aspergillus fumigatus (Sartorya fumigata)
Length = 2462
Score = 31.9 bits (69), Expect = 8.2
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +2
Query: 173 LRIFTYDIMLDTXESLATNFSNLNSGLRETAL 268
+RI T D+ T ESL TN S +N LRE L
Sbjct: 1648 MRIVTLDVESATTESLGTNASAINMALREITL 1679
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 430,803,768
Number of Sequences: 1657284
Number of extensions: 7653685
Number of successful extensions: 16138
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 15796
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16137
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 29273652170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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