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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0499.Seq
         (499 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g02140.1 68414.m00140 mago nashi family protein similar to Ma...   103   7e-23
At5g37470.1 68418.m04509 hypothetical protein contains Pfam PF04...    30   0.75 

>At1g02140.1 68414.m00140 mago nashi family protein similar to Mago
           Nashi, Genbank Accession Number U03559; contains Pfam
           PF02792: Mago nashi protein domain
          Length = 150

 Score =  103 bits (247), Expect = 7e-23
 Identities = 46/70 (65%), Positives = 55/70 (78%)
 Frame = +3

Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434
           GKLRYANNSNYKNDT+IRKE ++ P V+ E KRI+ +SEI+ E    WP+PDRVG  ELE
Sbjct: 34  GKLRYANNSNYKNDTIIRKEVFLTPAVLKECKRIVSESEILKEDDNNWPEPDRVGKQELE 93

Query: 435 IVIGEEHIXF 464
           IV+G EHI F
Sbjct: 94  IVLGNEHISF 103



 Score = 60.1 bits (139), Expect = 8e-10
 Identities = 23/29 (79%), Positives = 27/29 (93%)
 Frame = +1

Query: 172 STDFYIRYYVGHXGKFGHEFLEFEFRPEG 258
           +T+FY+RYYVGH GKFGHEFLEFEFR +G
Sbjct: 6   ATEFYLRYYVGHKGKFGHEFLEFEFREDG 34


>At5g37470.1 68418.m04509 hypothetical protein contains Pfam PF04510
           : Family of unknown function (DUF577)); common family
           comprised of  At5g37410, At5g37400, At5g37920,
           At5g37460, At5g37650, At5g37470, At5g37420, At5g37430
          Length = 629

 Score = 30.3 bits (65), Expect = 0.75
 Identities = 13/34 (38%), Positives = 22/34 (64%)
 Frame = -2

Query: 234 EKFVAKLSXVSNIISYVKIRRHFAQSFXFILENF 133
           EKFV +     N+ +Y K++RHF  +F F +++F
Sbjct: 257 EKFVLR---DKNLYNYTKVQRHFVMTFMFKIKDF 287


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,416,767
Number of Sequences: 28952
Number of extensions: 172820
Number of successful extensions: 333
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 325
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 333
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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