BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0499.Seq (499 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g02140.1 68414.m00140 mago nashi family protein similar to Ma... 103 7e-23 At5g37470.1 68418.m04509 hypothetical protein contains Pfam PF04... 30 0.75 >At1g02140.1 68414.m00140 mago nashi family protein similar to Mago Nashi, Genbank Accession Number U03559; contains Pfam PF02792: Mago nashi protein domain Length = 150 Score = 103 bits (247), Expect = 7e-23 Identities = 46/70 (65%), Positives = 55/70 (78%) Frame = +3 Query: 255 GKLRYANNSNYKNDTMIRKEAYVHPCVMDELKRIIIDSEIMHEXXRLWPQPDRVGWXELE 434 GKLRYANNSNYKNDT+IRKE ++ P V+ E KRI+ +SEI+ E WP+PDRVG ELE Sbjct: 34 GKLRYANNSNYKNDTIIRKEVFLTPAVLKECKRIVSESEILKEDDNNWPEPDRVGKQELE 93 Query: 435 IVIGEEHIXF 464 IV+G EHI F Sbjct: 94 IVLGNEHISF 103 Score = 60.1 bits (139), Expect = 8e-10 Identities = 23/29 (79%), Positives = 27/29 (93%) Frame = +1 Query: 172 STDFYIRYYVGHXGKFGHEFLEFEFRPEG 258 +T+FY+RYYVGH GKFGHEFLEFEFR +G Sbjct: 6 ATEFYLRYYVGHKGKFGHEFLEFEFREDG 34 >At5g37470.1 68418.m04509 hypothetical protein contains Pfam PF04510 : Family of unknown function (DUF577)); common family comprised of At5g37410, At5g37400, At5g37920, At5g37460, At5g37650, At5g37470, At5g37420, At5g37430 Length = 629 Score = 30.3 bits (65), Expect = 0.75 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = -2 Query: 234 EKFVAKLSXVSNIISYVKIRRHFAQSFXFILENF 133 EKFV + N+ +Y K++RHF +F F +++F Sbjct: 257 EKFVLR---DKNLYNYTKVQRHFVMTFMFKIKDF 287 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,416,767 Number of Sequences: 28952 Number of extensions: 172820 Number of successful extensions: 333 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 325 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 333 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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