Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0497.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g43010.1 68418.m05245 26S proteasome AAA-ATPase subunit (RPT4... 75 3e-14
At1g45000.1 68414.m05158 26S proteasome regulatory complex subun... 75 3e-14
At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2... 29 1.7
At2g20140.1 68415.m02353 26S protease regulatory complex subunit... 29 1.7
At5g48660.1 68418.m06022 expressed protein ; expression supporte... 27 5.3
At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit, puta... 27 7.0
At1g78700.1 68414.m09173 brassinosteroid signalling positive reg... 27 7.0
>At5g43010.1 68418.m05245 26S proteasome AAA-ATPase subunit (RPT4a)
gb|AAF22524.1
Length = 399
Score = 74.9 bits (176), Expect = 3e-14
Identities = 43/120 (35%), Positives = 61/120 (50%)
Frame = +2
Query: 104 REKAFQDYRKKLMEXKEVESRLKEGRDXLKDLTKQYDXSENDLKALQSVGX*LXKS*NSS 283
R A +YRKKL++ KE+ESR++ R+ L+ K+++ +E+DLK+LQSVG + +
Sbjct: 11 RTAAVSEYRKKLLQHKELESRVRTARENLRGAKKEFNKTEDDLKSLQSVGQIIGEVLRPL 70
Query: 284 QXXXXXXXXXXXXXXXXXVVGNLTKXCLKEEHXFALDMXXLTIMXXLXXEVDPLVYNMSH 463
+ K L LDM LTIM L EVDP+VYNM H
Sbjct: 71 DNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLH 130
Score = 58.0 bits (134), Expect = 3e-09
Identities = 26/43 (60%), Positives = 32/43 (74%)
Frame = +1
Query: 256 IVGEVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGTXV 384
I+GEVL+ L E+ IVKA++GPRYVVGCR +DK L GT V
Sbjct: 62 IIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRV 104
>At1g45000.1 68414.m05158 26S proteasome regulatory complex subunit
p42D, putative similar to 26S proteasome regulatory
complex subunit p42D [Drosophila melanogaster]
gi|6434958|gb|AAF08391
Length = 399
Score = 74.9 bits (176), Expect = 3e-14
Identities = 44/120 (36%), Positives = 60/120 (50%)
Frame = +2
Query: 104 REKAFQDYRKKLMEXKEVESRLKEGRDXLKDLTKQYDXSENDLKALQSVGX*LXKS*NSS 283
R A DYRKKL+ KE+ESR++ R+ L+ K+++ +E+DLK+LQSVG + +
Sbjct: 11 RTAAVTDYRKKLLHHKELESRVRTARENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPL 70
Query: 284 QXXXXXXXXXXXXXXXXXVVGNLTKXCLKEEHXFALDMXXLTIMXXLXXEVDPLVYNMSH 463
+ K L LDM LTIM L EVDP+VYNM H
Sbjct: 71 DNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLH 130
Score = 58.0 bits (134), Expect = 3e-09
Identities = 26/43 (60%), Positives = 32/43 (74%)
Frame = +1
Query: 256 IVGEVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGTXV 384
I+GEVL+ L E+ IVKA++GPRYVVGCR +DK L GT V
Sbjct: 62 IIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRV 104
>At4g29040.1 68417.m04153 26S proteasome AAA-ATPase subunit (RPT2a)
almost identical to 26S proteasome AAA-ATPase subunit
RPT2a (GI:6652880) {Arabidopsis thaliana}; Drosophila
melanogaster 26S proteasome subunit 4 ATPase,
PID:g1066065
Length = 443
Score = 29.1 bits (62), Expect = 1.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 259 VGEVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGTXV 384
VG + + + E IV ++ GP Y VG +DK L+ G +
Sbjct: 112 VGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSI 153
>At2g20140.1 68415.m02353 26S protease regulatory complex subunit 4,
putative similar to Swiss-Prot:P48601 26S protease
regulatory subunit 4 (P26S4) [Drosophila melanogaster]
Length = 443
Score = 29.1 bits (62), Expect = 1.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 259 VGEVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGTXV 384
VG + + + E IV ++ GP Y VG +DK L+ G +
Sbjct: 112 VGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSI 153
>At5g48660.1 68418.m06022 expressed protein ; expression supported
by MPSS
Length = 219
Score = 27.5 bits (58), Expect = 5.3
Identities = 12/53 (22%), Positives = 29/53 (54%)
Frame = +2
Query: 86 SDMEPLREKAFQDYRKKLMEXKEVESRLKEGRDXLKDLTKQYDXSENDLKALQ 244
++++ EKA ++ ++ ++ + RLK+ K+ K+ + +E + ALQ
Sbjct: 137 TELKEKEEKASKEIKQLQVKLSSITERLKKAETESKEKEKKLETAETHVTALQ 189
>At5g20000.1 68418.m02380 26S proteasome AAA-ATPase subunit,
putative almost identical to 26S proteasome AAA-ATPase
subunit RPT6a GI:6652888 from [Arabidopsis thaliana];
almost identical to a member of conserved Sug1 CAD
family AtSUG1 GI:13537115 from [Arabidopsis thaliana]
Length = 419
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 259 VGEVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGTXV 384
VGEV+K + + K +VK +YVV + +D L T V
Sbjct: 85 VGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKLTPSTRV 126
>At1g78700.1 68414.m09173 brassinosteroid signalling positive
regulator-related contains similarity to BZR1 protein
[Arabidopsis thaliana] gi|20270971|gb|AAM18490
Length = 325
Score = 27.1 bits (57), Expect = 7.0
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +1
Query: 265 EVLKQLTEEKFIVKATNGPRYVVGCRRXLDKXMLKGGT 378
EVLK L E + +G Y GC R +++ + GG+
Sbjct: 50 EVLKALCNEAGWIVEPDGTTYRKGCSRPVERMEIGGGS 87
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,375,218
Number of Sequences: 28952
Number of extensions: 129782
Number of successful extensions: 273
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 265
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 271
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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