Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0496.Seq
(449 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 32 0.21
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 31 0.48
At2g16140.1 68415.m01850 expressed protein contains similarity t... 29 1.1
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 29 1.1
At1g31460.1 68414.m03852 expressed protein 29 1.9
At4g33390.1 68417.m04746 hypothetical protein contains Pfam prof... 28 2.5
At1g56660.1 68414.m06516 expressed protein 28 2.5
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 28 3.4
At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila... 28 3.4
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 3.4
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 27 4.4
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 27 4.4
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 27 4.4
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 27 5.9
At1g24150.1 68414.m03047 formin homology 2 domain-containing pro... 27 7.7
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 31.9 bits (69), Expect = 0.21
Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -1
Query: 269 ERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREK-ERVWKNQKKGTASSRLSPSR 99
E + REV +K+ ++ T K +RT D R + ER + SSR SP R
Sbjct: 60 EGERDREVGDKRRRSGREDTEKRRRTRTDDERYSRGRHERSTSPSDRSHRSSRRSPER 117
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 30.7 bits (66), Expect = 0.48
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = -1
Query: 305 DKFSIGKNGISAERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 135
DK K+ E+ K +E KEKK K K K K ++EKER K +K
Sbjct: 67 DKEKAAKDKKEKEK-KDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERK 122
Score = 28.3 bits (60), Expect = 2.5
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -1
Query: 260 KSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 87
K ++ KE+K K K K K+ ++EKER K +K S + R ++P
Sbjct: 86 KEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAAARYRILSP 143
Score = 28.3 bits (60), Expect = 2.5
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = -1
Query: 269 ERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSR-LSP 105
++ K R+ KEKK K K K ++ ++ KE+ K + + A R LSP
Sbjct: 88 KKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAAARYRILSP 143
>At2g16140.1 68415.m01850 expressed protein contains similarity to
hypothetical proteins
Length = 311
Score = 29.5 bits (63), Expect = 1.1
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -1
Query: 269 ERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 132
E SR + K +KA +KT T D N E + +W+ ++K
Sbjct: 210 EEAMSRPIGVKAAKAKAKKTVTKTTTVEDKGNAMLEIQSIWEIKQK 255
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 29.5 bits (63), Expect = 1.1
Identities = 21/62 (33%), Positives = 28/62 (45%)
Frame = -1
Query: 272 AERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWV 93
+E ++ KEK+ KA KTTK +R +E +R K KG S R S
Sbjct: 275 SESATTKPQKEKEPKALKDKTTKAERRAI------QEAQRAAKAAAKGEGSRRADESGRA 328
Query: 92 NP 87
NP
Sbjct: 329 NP 330
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 28.7 bits (61), Expect = 1.9
Identities = 18/65 (27%), Positives = 32/65 (49%)
Frame = -1
Query: 269 ERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVN 90
++TK + KEK+ + ++ A PP+ E E+ K + K + +LSPS+ +
Sbjct: 155 QQTKKKLPKEKRRIVS--PSSSSSIDLATPPSTDSEPEKKSKPKSKSSWFDKLSPSKLIG 212
Query: 89 PA*RS 75
RS
Sbjct: 213 SIWRS 217
>At4g33390.1 68417.m04746 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 779
Score = 28.3 bits (60), Expect = 2.5
Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = -1
Query: 305 DKFSIGKNGISAERTKSREVKEKKSK--ANMRKTTKGKRTTADPPNIQREKE 156
+K GK G+ E K REV EKK K ++ K+ +G + ++ E E
Sbjct: 697 EKAKEGKLGVEQELRKWREVSEKKRKNGSSHGKSIQGSKEKEAETSVSNETE 748
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 28.3 bits (60), Expect = 2.5
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = -1
Query: 287 KNGISAERTKSREVKEKK----SKANMRKTTKGKRTTADPPNIQREKE 156
K+ E K + KEKK SK+N K KGK+ + ++++E E
Sbjct: 182 KDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDE 229
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 27.9 bits (59), Expect = 3.4
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = -1
Query: 260 KSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKG 129
+ RE KEKKSK + K ++ D Q++KE K ++KG
Sbjct: 25 EKREGKEKKSKDRSKDKQKERKEKKDKHKDQKDKE---KGKEKG 65
>At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar
to RNA helicase GB:A57514 GI:897915 from [Rattus
norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 989
Score = 27.9 bits (59), Expect = 3.4
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = -1
Query: 269 ERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 132
ER R ++K R +GK D +REKE+ K QKK
Sbjct: 63 ERDSKRRGEDKGRGRRERDRDRGKYLKRDRERREREKEKGRKKQKK 108
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 27.9 bits (59), Expect = 3.4
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 293 WRTCRGSYVLHTFPLHRIFRIRNLCCMSRRCR 388
W TC YVL+ +P+ I+ L C ++ CR
Sbjct: 646 WTTCPYCYVLYEYPI--IYEESVLKCQTKSCR 675
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 27.5 bits (58), Expect = 4.4
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = -1
Query: 272 AERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 138
AE K R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 455 AEEAK-RKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 497
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 27.5 bits (58), Expect = 4.4
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = -1
Query: 272 AERTKSREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 138
AE K R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 408 AEEAK-RKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 450
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 27.5 bits (58), Expect = 4.4
Identities = 23/94 (24%), Positives = 42/94 (44%)
Frame = -1
Query: 413 RGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSIGKNGISAERTKSREVKEKK 234
R SE ++ +DS+ R H++ GK +R + RE +E+K
Sbjct: 91 RRSERRKSSDSEDDVEEEDERDKRRVNEKERGHREHER-DRGK-----DRKRDREREERK 144
Query: 233 SKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 132
K R+ + +R + +REKERV + +++
Sbjct: 145 DKEREREKDRERR---EREREEREKERVKERERR 175
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 27.1 bits (57), Expect = 5.9
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 12/51 (23%)
Frame = -1
Query: 254 REVKEKKSKANMRKTTKGKRTTADP------------PNIQREKERVWKNQ 138
+E KEKK+K + K+ + KR D P Q++K R WKN+
Sbjct: 612 KERKEKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNE 662
>At1g24150.1 68414.m03047 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 725
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = -2
Query: 349 KNTVKRKSMKNVGPTTSSPLAR 284
KN + KS K GP T +PL R
Sbjct: 182 KNVIYSKSKKKSGPVTETPLLR 203
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,168,870
Number of Sequences: 28952
Number of extensions: 75782
Number of successful extensions: 395
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 362
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 390
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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