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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0489.Seq
         (399 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...    71   5e-14
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...    71   5e-14
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...    71   5e-14
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    33   0.016
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    27   1.1  
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|...    25   4.3  
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe...    25   4.3  
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar...    24   10.0 

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score = 71.3 bits (167), Expect = 5e-14
 Identities = 34/49 (69%), Positives = 37/49 (75%)
 Frame = -3

Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251
           VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV     G  KVTK
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score = 71.3 bits (167), Expect = 5e-14
 Identities = 34/49 (69%), Positives = 37/49 (75%)
 Frame = -3

Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251
           VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV     G  KVTK
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score = 71.3 bits (167), Expect = 5e-14
 Identities = 34/49 (69%), Positives = 37/49 (75%)
 Frame = -3

Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251
           VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV     G  KVTK
Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 33.1 bits (72), Expect = 0.016
 Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
 Frame = -3

Query: 385 PLCVEXFQEFPPLGRXAVRDMRQTXAVG-VIKAVN 284
           P+C+E F+++  +GR  +RD   T AVG V+K ++
Sbjct: 628 PVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 27.1 bits (57), Expect = 1.1
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = -3

Query: 385 PLCVEXFQEFPPLGRXAVRDMRQTXAVGVIK 293
           PLC+   +E P LGR  +R    T A G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 885

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = -1

Query: 228 QEVARAVNSTIFXTTAILHSPKGVSKEKRATNSFL 124
           + +A A+N +I  TT +    K +  E+ ++ SFL
Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403


>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 972

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 13/32 (40%), Positives = 14/32 (43%)
 Frame = -1

Query: 162 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 67
           G S E     S+   IFY       FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793


>SPAC23A1.04c |mnl1||alpha mannosidase-like
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = +1

Query: 142 PFFLRNTFR*MKYRSCXKNCAVNSSSYFLPLVAFSA 249
           PF   N  + + YR   +NCA  +SS  L     +A
Sbjct: 184 PFARINLMKGVAYREVTENCAAAASSLVLEFSMLTA 219


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,289,912
Number of Sequences: 5004
Number of extensions: 19638
Number of successful extensions: 60
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 60
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 60
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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