BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0489.Seq (399 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 71 5e-14 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 71 5e-14 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 71 5e-14 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 33 0.016 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 27 1.1 SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 25 4.3 SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 4.3 SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 24 10.0 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 71.3 bits (167), Expect = 5e-14 Identities = 34/49 (69%), Positives = 37/49 (75%) Frame = -3 Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251 VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV G KVTK Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 71.3 bits (167), Expect = 5e-14 Identities = 34/49 (69%), Positives = 37/49 (75%) Frame = -3 Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251 VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV G KVTK Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 71.3 bits (167), Expect = 5e-14 Identities = 34/49 (69%), Positives = 37/49 (75%) Frame = -3 Query: 397 VPSKPLCVEXFQEFPPLGRXAVRDMRQTXAVGVIKAVNFKEAGGGKVTK 251 VPSKP+CVE F ++ PLGR AVRDMRQT AVGVIKAV G KVTK Sbjct: 403 VPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAKVTK 451 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 33.1 bits (72), Expect = 0.016 Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 1/35 (2%) Frame = -3 Query: 385 PLCVEXFQEFPPLGRXAVRDMRQTXAVG-VIKAVN 284 P+C+E F+++ +GR +RD T AVG V+K ++ Sbjct: 628 PVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 27.1 bits (57), Expect = 1.1 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = -3 Query: 385 PLCVEXFQEFPPLGRXAVRDMRQTXAVGVIK 293 PLC+ +E P LGR +R T A G++K Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589 >SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 885 Score = 25.0 bits (52), Expect = 4.3 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = -1 Query: 228 QEVARAVNSTIFXTTAILHSPKGVSKEKRATNSFL 124 + +A A+N +I TT + K + E+ ++ SFL Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403 >SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 25.0 bits (52), Expect = 4.3 Identities = 13/32 (40%), Positives = 14/32 (43%) Frame = -1 Query: 162 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 67 G S E S+ IFY FYNL KV Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793 >SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 787 Score = 23.8 bits (49), Expect = 10.0 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +1 Query: 142 PFFLRNTFR*MKYRSCXKNCAVNSSSYFLPLVAFSA 249 PF N + + YR +NCA +SS L +A Sbjct: 184 PFARINLMKGVAYREVTENCAAAASSLVLEFSMLTA 219 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,289,912 Number of Sequences: 5004 Number of extensions: 19638 Number of successful extensions: 60 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 60 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 60 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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