BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0483.Seq (528 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 27 2.3 SPAC27F1.06c |||FKBP-type peptidyl-prolyl cis-trans isomerase |S... 26 3.0 SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 3.0 SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 26 4.0 SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 25 7.0 SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 7.0 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 25 7.0 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 25 9.2 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 25 9.2 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 25 9.2 SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 25 9.2 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 26.6 bits (56), Expect = 2.3 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = -3 Query: 259 VLIGKNGISAERTKSKRGERKEVEGEYEK 173 V I K+G+ E T ++ R+++E EYE+ Sbjct: 517 VNITKDGLELEETDEEKAAREKLEKEYEE 545 >SPAC27F1.06c |||FKBP-type peptidyl-prolyl cis-trans isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 362 Score = 26.2 bits (55), Expect = 3.0 Identities = 11/44 (25%), Positives = 24/44 (54%) Frame = -1 Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 94 ++ ++E KEKK+ ++ + GK+ +P + E+ K+ K Sbjct: 206 DKLKKEEKEKKTAVDVSDSVNGKKRKTEPAGEGEQTEKKSKSTK 249 >SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 3.0 Identities = 17/50 (34%), Positives = 25/50 (50%) Frame = -1 Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASS 76 ER Q+EV+E K+K N + KRT + E + ++KK SS Sbjct: 41 ERRQKEVEEAKAKQNDKLLDLRKRTFTN----HLENNELADDEKKSQVSS 86 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 25.8 bits (54), Expect = 4.0 Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 5/58 (8%) Frame = -1 Query: 216 QREVKEKKSKANMRKTTKGKRTTADPPNIQREKE-----RVWKNQKKGTASSRLSPSR 58 +R+ KEKK ++ K K A QR +E R K KKG A + PS+ Sbjct: 174 RRKEKEKKKSSDAWKERKDNEKKAMLMRQQRREENLKKRRESKKSKKGKAPKKKKPSK 231 >SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 25.0 bits (52), Expect = 7.0 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = -1 Query: 204 KEKKSKANMRKTTKGKRTTADPPNIQREKE 115 K+K + N +K KGK T + P ++E Sbjct: 518 KDKDASRNNKKNKKGKHTQIEDPTAASKEE 547 >SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 25.0 bits (52), Expect = 7.0 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = -1 Query: 198 KKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASS 76 K+ K N+ T G T N +EK R WK + + +S Sbjct: 160 KERKINIELTAGGGGKTEARMNKIKEKNRKWKEEMRQRVAS 200 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 25.0 bits (52), Expect = 7.0 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = -1 Query: 147 ADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 46 A P I+++ R+W + K+G S L R ++P Sbjct: 177 AAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSP 210 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 24.6 bits (51), Expect = 9.2 Identities = 26/144 (18%), Positives = 56/144 (38%), Gaps = 1/144 (0%) Frame = -1 Query: 522 KKXTNEXRRKI*KXELTKDKEIKISISKAEGLTLE-KEGYKSDYDRNEYEERGSEHQEDN 346 KK + + K+ K E + +E + +A LE K ++ R E + + ++ Sbjct: 596 KKRDKKKQLKLAKEEERQRREAERLAEQAAQKALEAKRQEEARKKREEQRLKREQEKKQQ 655 Query: 345 DSDXXXXXXXXXXXXXXXXXXXERRTHDKFSLARTXLVPSERNQREVKEKKSKANMRKTT 166 + + +++ D+ +AR + E +R ++E+K + + K Sbjct: 656 ELERQKREEKQKQKEREKKLKKQQQEADREKMAREQRLREEEEKRILEERKRREKLDKEE 715 Query: 165 KGKRTTADPPNIQREKERVWKNQK 94 + +R EKER + K Sbjct: 716 EERRRRELLEKESEEKERRLREAK 739 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 24.6 bits (51), Expect = 9.2 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -3 Query: 499 AKDLKXRAHERQRNKDIHLESRRIDFRKRRIQ 404 A+ LK RAH+R + LES + D + R++Q Sbjct: 350 AQKLKKRAHDRLATAERRLESAKED-QARKLQ 380 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 24.6 bits (51), Expect = 9.2 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = -1 Query: 453 ISISKAEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 337 + + EG L EG + DY +N +R + D+D+D Sbjct: 61 LDVEDEEGEDLFGEGMERDYQQNLELDRYDIEELDDDND 99 >SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 578 Score = 24.6 bits (51), Expect = 9.2 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = -1 Query: 204 KEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSR 73 KEKKSK +K K K +AD I ++ + K KKG + ++ Sbjct: 71 KEKKSKHEHKKHKKDK-PSADKDRISKKDK---KKSKKGKSKTK 110 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,419,807 Number of Sequences: 5004 Number of extensions: 22095 Number of successful extensions: 97 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 97 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 216376042 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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