Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0483.Seq
(528 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53800.1 68418.m06685 expressed protein 32 0.27
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 31 0.36
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 30 0.84
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 30 0.84
At1g56660.1 68414.m06516 expressed protein 30 1.1
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 29 1.5
At2g16140.1 68415.m01850 expressed protein contains similarity t... 29 1.5
At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing ... 29 1.9
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 29 1.9
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 29 1.9
At5g19310.1 68418.m02301 homeotic gene regulator, putative simil... 29 2.6
At3g29075.1 68416.m03637 glycine-rich protein 29 2.6
At1g10890.1 68414.m01251 F-box family protein contains Pfam PF00... 29 2.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 28 3.4
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 28 3.4
At1g31460.1 68414.m03852 expressed protein 28 3.4
At1g21660.1 68414.m02711 expressed protein low similarity to SP|... 28 3.4
At3g02810.1 68416.m00273 protein kinase family protein contains ... 28 4.5
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 28 4.5
At3g28110.1 68416.m03508 hypothetical protein 27 5.9
At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase, p... 27 5.9
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p... 27 5.9
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 7.8
At3g04990.1 68416.m00542 hypothetical protein 27 7.8
At2g24310.1 68415.m02906 expressed protein 27 7.8
At1g65190.1 68414.m07391 protein kinase family protein contains ... 27 7.8
At1g02040.1 68414.m00124 zinc finger (C2H2 type) family protein ... 27 7.8
>At5g53800.1 68418.m06685 expressed protein
Length = 351
Score = 31.9 bits (69), Expect = 0.27
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -1
Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 91
+R +RE +EK+ K R+ K KR +D ++ KE+ K +K
Sbjct: 136 KRKEREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSEK 180
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 31.5 bits (68), Expect = 0.36
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 7/52 (13%)
Frame = -1
Query: 231 PSERNQREVKEK--KSKANMR--KTTKGKRTTADPPNI---QREKERVWKNQ 97
P ER +++ KEK KSK + R K K K + P+ Q++K R WKN+
Sbjct: 611 PKERKEKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNE 662
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 30.3 bits (65), Expect = 0.84
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = -1
Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 94
E+ +E KEKK K K K K ++EKER K +K
Sbjct: 79 EKKDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERK 122
Score = 29.1 bits (62), Expect = 1.9
Identities = 17/60 (28%), Positives = 28/60 (46%)
Frame = -1
Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 46
E +++ KE+K K K K K+ ++EKER K +K S + R ++P
Sbjct: 84 EEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAAARYRILSP 143
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 30.3 bits (65), Expect = 0.84
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -1
Query: 213 REVKEKKSKANMRKTTKGKRTTADPPNIQREK-ERVWKNQKKGTASSRLSPSR 58
REV +K+ ++ T K +RT D R + ER + SSR SP R
Sbjct: 65 REVGDKRRRSGREDTEKRRRTRTDDERYSRGRHERSTSPSDRSHRSSRRSPER 117
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 29.9 bits (64), Expect = 1.1
Identities = 26/110 (23%), Positives = 48/110 (43%), Gaps = 1/110 (0%)
Frame = -1
Query: 441 KAEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHD 262
K E L EKEG K ++ E +E G E +++ +D ++
Sbjct: 122 KHEELEEEKEGKKKK-NKKEKDESGPE-EKNKKADKEKKHEDVSQEKEELEEEDGKKNKK 179
Query: 261 KFSLARTXLVPSERNQREVKEKK-SKANMRKTTKGKRTTADPPNIQREKE 115
K ++ ++E K+K+ SK+N K KGK+ + ++++E E
Sbjct: 180 KEKDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDE 229
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -1
Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKG 88
++ +RE KEKKSK + K ++ D Q++KE K ++KG
Sbjct: 23 DKEKREGKEKKSKDRSKDKQKERKEKKDKHKDQKDKE---KGKEKG 65
>At2g16140.1 68415.m01850 expressed protein contains similarity to
hypothetical proteins
Length = 311
Score = 29.5 bits (63), Expect = 1.5
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 4/66 (6%)
Frame = -1
Query: 276 RRTHDKFSLARTXLVPS----ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERV 109
RR DK + T PS E R + K +KA +KT T D N E + +
Sbjct: 190 RRKCDKAAQPSTSQPPSHGVEEAMSRPIGVKAAKAKAKKTVTKTTTVEDKGNAMLEIQSI 249
Query: 108 WKNQKK 91
W+ ++K
Sbjct: 250 WEIKQK 255
>At5g09880.1 68418.m01142 RNA recognition motif (RRM)-containing
protein
Length = 527
Score = 29.1 bits (62), Expect = 1.9
Identities = 26/99 (26%), Positives = 40/99 (40%)
Frame = -1
Query: 420 EKEGYKSDYDRNEYEERGSEHQEDNDSDXXXXXXXXXXXXXXXXXXXERRTHDKFSLART 241
E+E ++S D++ ++ E D +SD ERR +K S +R+
Sbjct: 76 ERERHRSSRDKDRERDKVREGSRDKESDRERSSKERDRSDRDKPRDRERREREKRSSSRS 135
Query: 240 XLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQR 124
E + EV E+ S+ R K ADP QR
Sbjct: 136 R---REEKEPEVVERGSR---RHRDKKDEPEADPERDQR 168
>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -1
Query: 231 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 115
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -1
Query: 231 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 115
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At5g19310.1 68418.m02301 homeotic gene regulator, putative similar to
SP|P25439 Homeotic gene regulator (Brahma protein)
{Drosophila melanogaster}; contains Pfam profiles
PF00271: Helicase conserved C-terminal domain, PF00176:
SNF2 family N-terminal domain
Length = 1064
Score = 28.7 bits (61), Expect = 2.6
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = -1
Query: 486 KXELTKDKEIKISISKAEGLTLEKEGYKSDYDRNEYEERGSEHQEDND 343
K + T K + SKAE + E + K + + EE G E +E+N+
Sbjct: 980 KRKRTDTKTRMSNGSKAEAVLSESDEEKEEEEEERKEESGKESEEENE 1027
>At3g29075.1 68416.m03637 glycine-rich protein
Length = 294
Score = 28.7 bits (61), Expect = 2.6
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -1
Query: 420 EKEGYKSDYDRNEYEERGSEHQEDNDSD 337
+KE YK +D ++Y+E+ + ++ ND D
Sbjct: 189 KKEQYKEHHDDDDYDEKKKKKKDYNDDD 216
>At1g10890.1 68414.m01251 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 592
Score = 28.7 bits (61), Expect = 2.6
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -1
Query: 228 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRL 70
+E N + V+E + K M + K + + +QR+KE + +K RL
Sbjct: 159 AEENLKRVEEAQRKEAMERQRKEEERYRELEELQRQKEEAMRRKKAEEEEERL 211
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.3 bits (60), Expect = 3.4
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -1
Query: 228 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 97
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 455 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 497
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 28.3 bits (60), Expect = 3.4
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -1
Query: 228 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 97
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 408 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 450
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 28.3 bits (60), Expect = 3.4
Identities = 16/68 (23%), Positives = 32/68 (47%)
Frame = -1
Query: 237 LVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSR 58
L P ++ ++ K+ + + ++ A PP+ E E+ K + K + +LSPS+
Sbjct: 150 LSPVKQQTKKKLPKEKRRIVSPSSSSSIDLATPPSTDSEPEKKSKPKSKSSWFDKLSPSK 209
Query: 57 WVNPA*RS 34
+ RS
Sbjct: 210 LIGSIWRS 217
>At1g21660.1 68414.m02711 expressed protein low similarity to
SP|O14976 Cyclin G-associated kinase (EC 2.7.1.-) {Homo
sapiens}; supporting cDNA gi|20466222|gb|AY099577.1|
Length = 523
Score = 28.3 bits (60), Expect = 3.4
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 264 DKFSLARTXLVPSERNQREVKEKKSKANMRKTTKGKRTTADPP 136
D F AR+ VP R E K++ +KT G PP
Sbjct: 311 DSFFSARSSSVPKSRTTTETTRKQASNAPKKTPNGVSNAKKPP 353
>At3g02810.1 68416.m00273 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 558
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = -1
Query: 474 TKDKEIKISISKAEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 337
T+D + S + L EKE + + ++EE S + D++SD
Sbjct: 385 TEDDKSSTSSGEESSLESEKESVSKNEYKKKHEEEDSSMESDDESD 430
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 27.9 bits (59), Expect = 4.5
Identities = 20/53 (37%), Positives = 24/53 (45%)
Frame = -1
Query: 204 KEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 46
KEK+ KA KTTK +R +E +R K KG S R S NP
Sbjct: 284 KEKEPKALKDKTTKAERRAI------QEAQRAAKAAAKGEGSRRADESGRANP 330
>At3g28110.1 68416.m03508 hypothetical protein
Length = 354
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 435 EGLTLEKEGYKSDYDRNEYEERGSEHQEDND 343
E + +++E + D NEYE+ +E ED D
Sbjct: 132 EDINVDEEDDIDEGDENEYEDEDNEEDEDMD 162
>At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 362
Score = 27.5 bits (58), Expect = 5.9
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +3
Query: 27 LSCFFKPDLPNEKDLDD 77
LSCF PD+P +DL D
Sbjct: 153 LSCFMAPDVPETEDLPD 169
>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF01480: PWI domain, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 899
Score = 27.5 bits (58), Expect = 5.9
Identities = 12/43 (27%), Positives = 21/43 (48%)
Frame = -1
Query: 465 KEIKISISKAEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 337
K I + K E + EK+ + +D+ + +R E + D D D
Sbjct: 716 KRISSTNPKEETIETEKQRSRRSHDKASHRDRERERERDRDRD 758
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = -1
Query: 225 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 91
E Q+E E K RK ++ K+ + Q+E K ++K
Sbjct: 747 EETQKEANESTKKERKRKKSESKKQSDGEEETQKEPSESTKKERK 791
>At3g04990.1 68416.m00542 hypothetical protein
Length = 227
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/55 (25%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = -1
Query: 519 KXTNEXRRKI*KXELTKDKEIKISISKAE--GLTLEKEGYKSDYDRNEYEERGSE 361
+ + +R++ TK KE+ +++ + + G LEK+ + + E EE+G E
Sbjct: 124 RHVQKRKREMEDETATKKKELSMTVDQIQESGKQLEKKSREVELKDKEIEEKGKE 178
>At2g24310.1 68415.m02906 expressed protein
Length = 322
Score = 27.1 bits (57), Expect = 7.8
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = -1
Query: 258 FSLARTXLVPSERNQREVKEKKSKANMRKTTKGKRTT--ADPPNIQREKERVWKNQKKGT 85
FS+A T LV S + + S++N++ ++ +TT + P++ N T
Sbjct: 113 FSMAETTLVSSSPSLPAENVRVSESNLKSSSSTAKTTPVSASPSVNARVSESNLNSSSST 172
Query: 84 ASSRLSPSRWV 52
+SPS V
Sbjct: 173 TPVSVSPSEKV 183
>At1g65190.1 68414.m07391 protein kinase family protein contains
similarity to protein kinases
Length = 396
Score = 27.1 bits (57), Expect = 7.8
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -1
Query: 432 GLTLEKEGYKSDYDRNEYEERGSEHQEDNDSD 337
GLTL GYKS ++ EE+ SE ++ D+D
Sbjct: 238 GLTLLL-GYKSYFEHYRGEEKESEEEDPEDTD 268
>At1g02040.1 68414.m00124 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 324
Score = 27.1 bits (57), Expect = 7.8
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = -1
Query: 465 KEIKISISKAEGLTLEKEGYKSDYDRNEYEERGSEHQEDNDS 340
K++K + + E+E YK D D N+ +E E +ED +
Sbjct: 173 KKVKGCFASQDKEEEEEEEYKEDDDDNDEDEDEEEDEEDKST 214
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,265,432
Number of Sequences: 28952
Number of extensions: 114530
Number of successful extensions: 700
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 687
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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