BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0481.Seq (429 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyc... 30 0.13 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 0.93 SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 27 1.6 SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizo... 27 1.6 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 27 1.6 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 2.2 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 5.0 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 25 5.0 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 24 8.7 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 24 8.7 >SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 150 Score = 30.3 bits (65), Expect = 0.13 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Frame = +2 Query: 146 NSLPP-HQAFPSPADRPPVIPTTXSARDRPPP 238 N +PP QA+ P PP +P ++ + PPP Sbjct: 96 NPMPPMSQAYRPPPTEPPTVPAYETSSEFPPP 127 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 0.93 Identities = 13/32 (40%), Positives = 15/32 (46%) Frame = +2 Query: 173 PSPADRPPVIPTTXSARDRPPPVSQPLYLNPS 268 P P PPV ++ A PPPV P PS Sbjct: 1044 PIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPS 1075 Score = 25.0 bits (52), Expect = 5.0 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = +2 Query: 173 PSPADRPPVIPTTXSARDRPPPVSQPLYLNPS 268 P P++ PPV + PPP + P PS Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPS 1223 >SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizosaccharomyces pombe|chr 2|||Manual Length = 953 Score = 26.6 bits (56), Expect = 1.6 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 149 SLPPHQAFPSPADRPPVIPTTXSARDRPPPVSQP 250 SL P + P+ PP IPT + PPV++P Sbjct: 360 SLTPLSSRPAHTSLPP-IPTVQTTSSNVPPVNRP 392 >SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 26.6 bits (56), Expect = 1.6 Identities = 20/76 (26%), Positives = 27/76 (35%), Gaps = 2/76 (2%) Frame = +2 Query: 14 SVPPAPLFARPNHHDHRFRME--QLVSEQFRHHGLNLXXXXXXXXXNSLPPHQAFPSPAD 187 S P P + P+ R E Q VS+ F N PP+ A PA Sbjct: 162 STPAVPYYGAPDQQQMGQRPEFAQNVSQGFAGQAPYNVRPGYGMPSNQKPPNFAPGMPAP 221 Query: 188 RPPVIPTTXSARDRPP 235 P + + S + PP Sbjct: 222 GPTAVSASPSLQSMPP 237 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 26.6 bits (56), Expect = 1.6 Identities = 12/39 (30%), Positives = 18/39 (46%) Frame = +2 Query: 149 SLPPHQAFPSPADRPPVIPTTXSARDRPPPVSQPLYLNP 265 SLP P +PPVIP + P + P++ +P Sbjct: 169 SLPSPNLPHQPISKPPVIPNLPKLQVHPNRLPHPIHNHP 207 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 2.2 Identities = 17/47 (36%), Positives = 22/47 (46%), Gaps = 6/47 (12%) Frame = +2 Query: 146 NSLPPHQAFPSPADRPPVIPTTXSA------RDRPPPVSQPLYLNPS 268 +SLPP +P PP P+ SA + PPP+SQ N S Sbjct: 169 SSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAPVANTS 215 Score = 25.4 bits (53), Expect = 3.8 Identities = 13/36 (36%), Positives = 16/36 (44%), Gaps = 2/36 (5%) Frame = +2 Query: 149 SLPPHQAFP--SPADRPPVIPTTXSARDRPPPVSQP 250 S PP + P SPA PP+ PP +QP Sbjct: 142 SAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.0 bits (52), Expect = 5.0 Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 2/37 (5%) Frame = +2 Query: 146 NSLPPHQAFPSPADRPPVIPTTXSARDRP--PPVSQP 250 ++ PP + P+P P +P SA P PP + P Sbjct: 1696 SAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732 Score = 24.6 bits (51), Expect = 6.6 Identities = 14/38 (36%), Positives = 16/38 (42%), Gaps = 1/38 (2%) Frame = +2 Query: 155 PPHQAFPSPADRPPVIPTTXSARDRPPPVSQ-PLYLNP 265 PP + P P PP+ SA P P S P NP Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.0 bits (52), Expect = 5.0 Identities = 11/32 (34%), Positives = 13/32 (40%) Frame = +2 Query: 155 PPHQAFPSPADRPPVIPTTXSARDRPPPVSQP 250 PP P+PA P +P PPP P Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 24.2 bits (50), Expect = 8.7 Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 5/46 (10%) Frame = +2 Query: 146 NSLPPHQAFPSPADRPPVIP-----TTXSARDRPPPVSQPLYLNPS 268 +S PP + A PP IP T S + PP P+ +NP+ Sbjct: 238 SSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPA 283 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 24.2 bits (50), Expect = 8.7 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 149 SLPPHQAFPSPADRPPVIPTTXSARDRPPP 238 S+PP +P P V PT+ S+ PPP Sbjct: 290 SIPPTGNSTTPVT-PTVPPTSTSSTSTPPP 318 Score = 24.2 bits (50), Expect = 8.7 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 149 SLPPHQAFPSPADRPPVIPTTXSARDRPPP 238 S+PP +P P V PT+ S+ PPP Sbjct: 344 SIPPTGNSTTPVT-PTVPPTSTSSTSTPPP 372 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,349,491 Number of Sequences: 5004 Number of extensions: 21624 Number of successful extensions: 86 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 68 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 154448264 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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