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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0476.Seq
         (548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   162   4e-41
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   162   4e-41
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    92   4e-20
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz...    26   3.2  
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c...    26   3.2  
SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|...    26   3.2  
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    25   5.6  
SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa...    25   5.6  
SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc...    25   5.6  
SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe...    25   7.3  
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    25   7.3  
SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l...    25   9.7  

>SPCP31B10.07 |eft202||translation elongation factor 2
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  162 bits (393), Expect = 4e-41
 Identities = 89/187 (47%), Positives = 109/187 (58%), Gaps = 5/187 (2%)
 Frame = +3

Query: 3    PIXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDF 182
            P+  S PVVSYRE+V+E S    LSKSPNKHNR+FM A+PM + L   I+ G VNPRDDF
Sbjct: 553  PLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDF 612

Query: 183  KTRARYLTEKYEYDVTEARKIWCFAP-----RVPAPTSWWIAPKEFSTSMKLRTLLXLDS 347
            K RAR + +++ +DVT+ARKIWCF P      V    +  +A         +        
Sbjct: 613  KVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASK 672

Query: 348  SGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSL 527
             GP+ +E    R C    FNI DV LH DAIHRGGGQIIPT RR +YA  L A P+    
Sbjct: 673  EGPMFEE--NLRSC---RFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEP 727

Query: 528  YIFCEIQ 548
                EIQ
Sbjct: 728  VFLVEIQ 734



 Score = 66.1 bits (154), Expect = 3e-12
 Identities = 30/45 (66%), Positives = 35/45 (77%)
 Frame = +2

Query: 257 PEGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAKEGVMAEENLR 391
           P+ TG N++VD +K V YLNEIKDSVV  F WA+KEG M EENLR
Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLR 682


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
            elongation factor 2 |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 842

 Score =  162 bits (393), Expect = 4e-41
 Identities = 89/187 (47%), Positives = 109/187 (58%), Gaps = 5/187 (2%)
 Frame = +3

Query: 3    PIXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDF 182
            P+  S PVVSYRE+V+E S    LSKSPNKHNR+FM A+PM + L   I+ G VNPRDDF
Sbjct: 553  PLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDF 612

Query: 183  KTRARYLTEKYEYDVTEARKIWCFAP-----RVPAPTSWWIAPKEFSTSMKLRTLLXLDS 347
            K RAR + +++ +DVT+ARKIWCF P      V    +  +A         +        
Sbjct: 613  KVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASK 672

Query: 348  SGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSL 527
             GP+ +E    R C    FNI DV LH DAIHRGGGQIIPT RR +YA  L A P+    
Sbjct: 673  EGPMFEE--NLRSC---RFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEP 727

Query: 528  YIFCEIQ 548
                EIQ
Sbjct: 728  VFLVEIQ 734



 Score = 66.1 bits (154), Expect = 3e-12
 Identities = 30/45 (66%), Positives = 35/45 (77%)
 Frame = +2

Query: 257 PEGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAKEGVMAEENLR 391
           P+ TG N++VD +K V YLNEIKDSVV  F WA+KEG M EENLR
Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLR 682


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 983

 Score = 92.3 bits (219), Expect = 4e-20
 Identities = 60/178 (33%), Positives = 86/178 (48%), Gaps = 9/178 (5%)
 Frame = +3

Query: 6    IXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFK 185
            I  SDPV  + ET  + S   C S +PNK NR+ M  +P+  G+  DI+ G+VN     K
Sbjct: 666  IRVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWPQK 725

Query: 186  TRARYLTEKYEYDVTEARKIWCFAPR------VPAPTSWWIAPKEFSTSMK---LRTLLX 338
              + +  + Y++D+  +R IW F P       +   T      K    S+K    +    
Sbjct: 726  RISEFFQKNYDWDLLASRSIWAFGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQW 785

Query: 339  LDSSGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 512
                GPL  E  ++ +     F + DV L  + I+RGGGQIIPT RR  Y+  LTA P
Sbjct: 786  GTREGPLCDET-IRNV----NFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFLTASP 838



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 4/51 (7%)
 Frame = +2

Query: 257 PEGTGPNILVDCSKGVQY----LNEIKDSVVXGFQWAAKEGVMAEENLRGV 397
           P+  G NIL D +         LN +K+ +  GFQW  +EG + +E +R V
Sbjct: 750 PDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDETIRNV 800


>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1253

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = +3

Query: 135 LPEDIDEGRVNPRDDFKT 188
           LPE IDEG+++  DD KT
Sbjct: 194 LPEKIDEGKLSDVDDGKT 211


>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 871

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +3

Query: 72  LSKSPNKHNRLFMKAQPMPDGL 137
           ++KS  KHN+L  K +P+P  L
Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264


>SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 578

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 11/24 (45%), Positives = 16/24 (66%)
 Frame = +2

Query: 455 PNHSNN*KMLVRMSANCSPRLMEP 526
           P+H+N   + +R+S N   RLMEP
Sbjct: 469 PSHANEDMVRLRISRNGEERLMEP 492


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 2/50 (4%)
 Frame = +1

Query: 166  IPAMTSRLA--LGILQKSTNTMLPKPVRFGALPRGYRPQHPGGLLQRSSV 309
            +P MT  +    G+  + T  M  +PV +G    G +PQ  G + Q + +
Sbjct: 1105 VPQMTGVMPQMTGVQVQKTGAMPQQPVNYGYQVAGMQPQATGIISQPTGI 1154


>SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
 Frame = -2

Query: 355 GPLESXHNR--VLNFIEVLNSFGAIHQDVGAGTLGAKHQILRASVTSYSYFS 206
           G L S HN   V+ + E+ +      ++V     G KH +LRA+  +Y YFS
Sbjct: 288 GLLVSSHNHPCVVEYSEISDEACKATENVD----GHKHLLLRAANIAYHYFS 335


>SPBC4C3.12 |sep1||fork head transcription factor
           Sep1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 663

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = +1

Query: 196 GILQKSTNTMLPKPVRFGALPRGYRPQHPGGLLQRSS 306
           G+   +TNT+    VR  +LP     ++P GLL + S
Sbjct: 408 GLKDGTTNTLQIPAVRLPSLPSSPTIKNPSGLLLKRS 444


>SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 230

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = -1

Query: 248 PNLTGFGNIVFVLFCKIPSAS 186
           PNL   GN +F L  ++PS+S
Sbjct: 174 PNLNSIGNKMFSLKSRVPSSS 194


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +3

Query: 36  RETVAEESDQLCLSKSPNKHNRLFMKAQP 122
           R T  EE +   L+KSP+ HNR+     P
Sbjct: 466 RLTDDEEREIRALAKSPDIHNRIIASMAP 494


>SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine
           ligase |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 788

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 10/29 (34%), Positives = 16/29 (55%)
 Frame = +3

Query: 411 YDVTLHTDAIHRGGGQIIPTTRRCLYACL 497
           +DV +  D +  G G IIP T++  +  L
Sbjct: 146 FDVVIKADGLAAGKGVIIPKTKKEAFEAL 174


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,347,606
Number of Sequences: 5004
Number of extensions: 48742
Number of successful extensions: 164
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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