BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0476.Seq (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 162 4e-41 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 162 4e-41 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 92 4e-20 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 3.2 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 3.2 SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 26 3.2 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 5.6 SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa... 25 5.6 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 25 5.6 SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 25 7.3 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 25 7.3 SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l... 25 9.7 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 162 bits (393), Expect = 4e-41 Identities = 89/187 (47%), Positives = 109/187 (58%), Gaps = 5/187 (2%) Frame = +3 Query: 3 PIXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDF 182 P+ S PVVSYRE+V+E S LSKSPNKHNR+FM A+PM + L I+ G VNPRDDF Sbjct: 553 PLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDF 612 Query: 183 KTRARYLTEKYEYDVTEARKIWCFAP-----RVPAPTSWWIAPKEFSTSMKLRTLLXLDS 347 K RAR + +++ +DVT+ARKIWCF P V + +A + Sbjct: 613 KVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASK 672 Query: 348 SGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSL 527 GP+ +E R C FNI DV LH DAIHRGGGQIIPT RR +YA L A P+ Sbjct: 673 EGPMFEE--NLRSC---RFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEP 727 Query: 528 YIFCEIQ 548 EIQ Sbjct: 728 VFLVEIQ 734 Score = 66.1 bits (154), Expect = 3e-12 Identities = 30/45 (66%), Positives = 35/45 (77%) Frame = +2 Query: 257 PEGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAKEGVMAEENLR 391 P+ TG N++VD +K V YLNEIKDSVV F WA+KEG M EENLR Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLR 682 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 162 bits (393), Expect = 4e-41 Identities = 89/187 (47%), Positives = 109/187 (58%), Gaps = 5/187 (2%) Frame = +3 Query: 3 PIXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDF 182 P+ S PVVSYRE+V+E S LSKSPNKHNR+FM A+PM + L I+ G VNPRDDF Sbjct: 553 PLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDF 612 Query: 183 KTRARYLTEKYEYDVTEARKIWCFAP-----RVPAPTSWWIAPKEFSTSMKLRTLLXLDS 347 K RAR + +++ +DVT+ARKIWCF P V + +A + Sbjct: 613 KVRARIMADEFGWDVTDARKIWCFGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASK 672 Query: 348 SGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSL 527 GP+ +E R C FNI DV LH DAIHRGGGQIIPT RR +YA L A P+ Sbjct: 673 EGPMFEE--NLRSC---RFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQEP 727 Query: 528 YIFCEIQ 548 EIQ Sbjct: 728 VFLVEIQ 734 Score = 66.1 bits (154), Expect = 3e-12 Identities = 30/45 (66%), Positives = 35/45 (77%) Frame = +2 Query: 257 PEGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAKEGVMAEENLR 391 P+ TG N++VD +K V YLNEIKDSVV F WA+KEG M EENLR Sbjct: 638 PDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLR 682 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 92.3 bits (219), Expect = 4e-20 Identities = 60/178 (33%), Positives = 86/178 (48%), Gaps = 9/178 (5%) Frame = +3 Query: 6 IXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFK 185 I SDPV + ET + S C S +PNK NR+ M +P+ G+ DI+ G+VN K Sbjct: 666 IRVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWPQK 725 Query: 186 TRARYLTEKYEYDVTEARKIWCFAPR------VPAPTSWWIAPKEFSTSMK---LRTLLX 338 + + + Y++D+ +R IW F P + T K S+K + Sbjct: 726 RISEFFQKNYDWDLLASRSIWAFGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQW 785 Query: 339 LDSSGPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHP 512 GPL E ++ + F + DV L + I+RGGGQIIPT RR Y+ LTA P Sbjct: 786 GTREGPLCDET-IRNV----NFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFLTASP 838 Score = 37.9 bits (84), Expect = 0.001 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 4/51 (7%) Frame = +2 Query: 257 PEGTGPNILVDCSKGVQY----LNEIKDSVVXGFQWAAKEGVMAEENLRGV 397 P+ G NIL D + LN +K+ + GFQW +EG + +E +R V Sbjct: 750 PDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDETIRNV 800 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 26.2 bits (55), Expect = 3.2 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +3 Query: 135 LPEDIDEGRVNPRDDFKT 188 LPE IDEG+++ DD KT Sbjct: 194 LPEKIDEGKLSDVDDGKT 211 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 26.2 bits (55), Expect = 3.2 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +3 Query: 72 LSKSPNKHNRLFMKAQPMPDGL 137 ++KS KHN+L K +P+P L Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264 >SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 578 Score = 26.2 bits (55), Expect = 3.2 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +2 Query: 455 PNHSNN*KMLVRMSANCSPRLMEP 526 P+H+N + +R+S N RLMEP Sbjct: 469 PSHANEDMVRLRISRNGEERLMEP 492 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 25.4 bits (53), Expect = 5.6 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 2/50 (4%) Frame = +1 Query: 166 IPAMTSRLA--LGILQKSTNTMLPKPVRFGALPRGYRPQHPGGLLQRSSV 309 +P MT + G+ + T M +PV +G G +PQ G + Q + + Sbjct: 1105 VPQMTGVMPQMTGVQVQKTGAMPQQPVNYGYQVAGMQPQATGIISQPTGI 1154 >SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 25.4 bits (53), Expect = 5.6 Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%) Frame = -2 Query: 355 GPLESXHNR--VLNFIEVLNSFGAIHQDVGAGTLGAKHQILRASVTSYSYFS 206 G L S HN V+ + E+ + ++V G KH +LRA+ +Y YFS Sbjct: 288 GLLVSSHNHPCVVEYSEISDEACKATENVD----GHKHLLLRAANIAYHYFS 335 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 25.4 bits (53), Expect = 5.6 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +1 Query: 196 GILQKSTNTMLPKPVRFGALPRGYRPQHPGGLLQRSS 306 G+ +TNT+ VR +LP ++P GLL + S Sbjct: 408 GLKDGTTNTLQIPAVRLPSLPSSPTIKNPSGLLLKRS 444 >SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 25.0 bits (52), Expect = 7.3 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = -1 Query: 248 PNLTGFGNIVFVLFCKIPSAS 186 PNL GN +F L ++PS+S Sbjct: 174 PNLNSIGNKMFSLKSRVPSSS 194 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 25.0 bits (52), Expect = 7.3 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 36 RETVAEESDQLCLSKSPNKHNRLFMKAQP 122 R T EE + L+KSP+ HNR+ P Sbjct: 466 RLTDDEEREIRALAKSPDIHNRIIASMAP 494 >SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 788 Score = 24.6 bits (51), Expect = 9.7 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +3 Query: 411 YDVTLHTDAIHRGGGQIIPTTRRCLYACL 497 +DV + D + G G IIP T++ + L Sbjct: 146 FDVVIKADGLAAGKGVIIPKTKKEAFEAL 174 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,347,606 Number of Sequences: 5004 Number of extensions: 48742 Number of successful extensions: 164 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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