Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0476.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 157 5e-39
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 100 1e-21
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 100 1e-21
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 93 8e-20
At5g23570.1 68418.m02765 XS domain-containing protein / XS zinc ... 31 0.51
At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid ... 30 0.89
At1g10095.1 68414.m01138 protein prenyltransferase alpha subunit... 25 0.89
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 30 1.2
At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contai... 29 1.5
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 2.0
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 28 3.6
At5g64220.1 68418.m08067 calmodulin-binding protein similar to a... 27 8.3
At2g44700.1 68415.m05563 kelch repeat-containing F-box family pr... 27 8.3
At2g02470.1 68415.m00186 PHD finger family protein contains Pfam... 27 8.3
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 157 bits (381), Expect = 5e-39
Identities = 85/186 (45%), Positives = 113/186 (60%), Gaps = 5/186 (2%)
Frame = +3
Query: 6 IXKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFK 185
I KSDPVVS+RETV + S + +SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K
Sbjct: 555 IIKSDPVVSFRETVCDRSTRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPK 614
Query: 186 TRARYLTEKYEYDVTEARKIWCFAPRVPAPTSWWIAPK--EFSTSMK---LRTLLXLDSS 350
R++ L E++ +D A+KIW F P P K ++ +K +
Sbjct: 615 IRSKILAEEFGWDKDLAKKIWAFGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKE 674
Query: 351 GPLRKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSLY 530
GPL +E ++ IC F + DV LH+DAIHRGGGQ+IPT RR +YA +TA P
Sbjct: 675 GPLAEEN-MRGIC----FEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPV 729
Query: 531 IFCEIQ 548
EIQ
Sbjct: 730 YMVEIQ 735
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 99.5 bits (237), Expect = 1e-21
Identities = 62/185 (33%), Positives = 91/185 (49%), Gaps = 7/185 (3%)
Frame = +3
Query: 15 SDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRA 194
+DPVVS+ ETV E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K
Sbjct: 670 ADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLG 729
Query: 195 RYLTEKYEYDVTEARKIWCFAPRVPAPTSWWIAPKEFSTSMKLRTLLXLDSSGPLRKELW 374
+ KY++D+ AR IW F P P + T + R L+ ++ W
Sbjct: 730 DFFRTKYDWDLLAARSIWAFGPDKQGPNI--LLDDTLPTEVD-RNLMMAVKDSIVQGFQW 786
Query: 375 LKR---ICVV----LXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSLYI 533
R +C + F I D + + +HRG GQ+IPT RR Y+ L A P
Sbjct: 787 GAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
Query: 534 FCEIQ 548
+ EIQ
Sbjct: 847 YVEIQ 851
Score = 45.6 bits (103), Expect = 2e-05
Identities = 36/103 (34%), Positives = 53/103 (51%), Gaps = 11/103 (10%)
Frame = +2
Query: 257 PEGTGPNILVDCSKGVQY----LNEIKDSVVXGFQWAAKEGVMAEENLRGVXIQHL*CNT 424
P+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R V + +
Sbjct: 751 PDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 810
Query: 425 PY*CHP*RWWPNHSNN*KML-----VRMSA--NCSPRLMEPVY 532
P P H + +M+ V SA +PRLMEPVY
Sbjct: 811 A----P---EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 99.5 bits (237), Expect = 1e-21
Identities = 62/185 (33%), Positives = 91/185 (49%), Gaps = 7/185 (3%)
Frame = +3
Query: 15 SDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRA 194
+DPVVS+ ETV E S C +++PNK N++ M A+P+ GL EDI+ G V+ + K
Sbjct: 670 ADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLG 729
Query: 195 RYLTEKYEYDVTEARKIWCFAPRVPAPTSWWIAPKEFSTSMKLRTLLXLDSSGPLRKELW 374
+ KY++D+ AR IW F P P + T + R L+ ++ W
Sbjct: 730 DFFRTKYDWDLLAARSIWAFGPDKQGPNI--LLDDTLPTEVD-RNLMMAVKDSIVQGFQW 786
Query: 375 LKR---ICVV----LXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSLYI 533
R +C + F I D + + +HRG GQ+IPT RR Y+ L A P
Sbjct: 787 GAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
Query: 534 FCEIQ 548
+ EIQ
Sbjct: 847 YVEIQ 851
Score = 45.6 bits (103), Expect = 2e-05
Identities = 36/103 (34%), Positives = 53/103 (51%), Gaps = 11/103 (10%)
Frame = +2
Query: 257 PEGTGPNILVDCSKGVQY----LNEIKDSVVXGFQWAAKEGVMAEENLRGVXIQHL*CNT 424
P+ GPNIL+D + + + +KDS+V GFQW A+EG + +E +R V + +
Sbjct: 751 PDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 810
Query: 425 PY*CHP*RWWPNHSNN*KML-----VRMSA--NCSPRLMEPVY 532
P P H + +M+ V SA +PRLMEPVY
Sbjct: 811 A----P---EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 846
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 93.5 bits (222), Expect = 8e-20
Identities = 59/182 (32%), Positives = 86/182 (47%), Gaps = 4/182 (2%)
Frame = +3
Query: 15 SDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRA 194
+DPVVS+ ETV E S C +++PNK N+L M A+P+ GL EDI+ G V+ +
Sbjct: 656 ADPVVSFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLG 715
Query: 195 RYLTEKYEYDVTEARKIWCFAPRVPAPTSWW--IAPKEFSTS--MKLRTLLXLDSSGPLR 362
+ KY++D+ AR IW F P P E + M ++ + R
Sbjct: 716 DFFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAR 775
Query: 363 KELWLKRICVVLXFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAHPVSWSLYIFCE 542
+ + F I D + + +HRG GQ+IPT RR Y+ L A P + E
Sbjct: 776 EGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVE 835
Query: 543 IQ 548
IQ
Sbjct: 836 IQ 837
Score = 41.9 bits (94), Expect = 3e-04
Identities = 35/103 (33%), Positives = 52/103 (50%), Gaps = 11/103 (10%)
Frame = +2
Query: 257 PEGTGPNILVDCSKGVQY----LNEIKDSVVXGFQWAAKEGVMAEENLRGVXIQHL*CNT 424
P+ G NIL+D + + + +KDS+V GFQW A+EG + +E +R V + +
Sbjct: 737 PDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 796
Query: 425 PY*CHP*RWWPNHSNN*KML-----VRMSA--NCSPRLMEPVY 532
P P H + +M+ V SA +PRLMEPVY
Sbjct: 797 A----P---EPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVY 832
>At5g23570.1 68418.m02765 XS domain-containing protein / XS zinc
finger domain-containing protein-related contains Pfam
profiles PF03468: XS domain, weak hit to PF03470: XS
zinc finger domain
Length = 625
Score = 31.1 bits (67), Expect = 0.51
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -1
Query: 293 SNPPGCWGRYPRGKAPNLTGFGNIV 219
SNPP WG +G+ N++G GN V
Sbjct: 68 SNPPRAWGGQQQGRGSNVSGRGNNV 92
>At5g07990.1 68418.m00930 flavonoid 3'-monooxygenase / flavonoid
3'-hydroxylase (F3'H) / cytochrome P450 75B1 (CYP75B1) /
transparent testa 7 protein (TT7) identical to SP|Q9SD85
Flavonoid 3'-monooxygenase (EC 1.14.13.21) (Flavonoid
3'-hydroxylase) (AtF3'H) (Cytochrome P450 75B1)
(TRANSPARENT TESTA 7 protein) {Arabidopsis thaliana};
similar to gi:10334806, gi:10334808
Length = 513
Score = 30.3 bits (65), Expect = 0.89
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = +2
Query: 242 DLVLCPEGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAKEGVMAEE 382
D L P G G I S G++ + + ++V GF W GV E+
Sbjct: 432 DFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEK 478
>At1g10095.1 68414.m01138 protein prenyltransferase alpha
subunit-related contains weak hit to Pfam profile
PF01239: Protein prenyltransferase alpha subunit repeat
Length = 431
Score = 25.0 bits (52), Expect(2) = 0.89
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +3
Query: 198 YLTEKYEYDVTEARKIW 248
Y+ YD TEARKIW
Sbjct: 279 YVKGSSAYDKTEARKIW 295
Score = 23.8 bits (49), Expect(2) = 0.89
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 360 RKELWLKRICVVLXFNIYDVTLHTDAIHRGGGQII 464
R+ LWL R + L + +Y H+DA G II
Sbjct: 312 REALWLHRRFLSLNWIMYFACNHSDASPETGESII 346
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 29.9 bits (64), Expect = 1.2
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = +3
Query: 54 ESDQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 149
E + LCL PN+H + + A+ +P LPE +
Sbjct: 44 EKENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At3g61740.1 68416.m06923 PHD finger family protein (ATX3) contains
Pfam domains PF00628: PHD-finger and PF00855: PWWP
domain; identical to cDNA trithorax 3 (ATX3) partial cds
GI:15217142
Length = 799
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/52 (26%), Positives = 22/52 (42%), Gaps = 3/52 (5%)
Frame = +1
Query: 388 AWCXDSTSMM*HSILMPSI---EVVAKSFQQLEDACTHVC*LLTPSHGACIS 534
AW + H + P++ ++ A SF + +C H C T H C S
Sbjct: 572 AWFRPEVGFLNHENMEPAVGLFKIPANSFLKTHGSCVHCCKCATHFHAMCAS 623
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 2.0
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +3
Query: 75 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 227
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 28.3 bits (60), Expect = 3.6
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 161 RESPR*LQDSRSVSYRKVRIRCYRSP*DLV 250
+E+PR DSRS S+R R C P +LV
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV 252
>At5g64220.1 68418.m08067 calmodulin-binding protein similar to
anther ethylene-upregulated calmodulin-binding protein
ER1 GI:11612392 from[Nicotiana tabacum]
Length = 1050
Score = 27.1 bits (57), Expect = 8.3
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 260 EGTGPNILVDCSKGVQYLNEIKDSVVXGFQWAAK 361
EG GPNIL + +GV +L + G+ WA K
Sbjct: 651 EGKGPNILDEDGQGVLHL-----AAALGYDWAIK 679
>At2g44700.1 68415.m05563 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif;
Length = 368
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = +3
Query: 144 DIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 251
D+D R ++ + ++ EK +V+E+++IWC
Sbjct: 294 DLDNARSVGIGEYYGKLAFIWEKPSLNVSESKEIWC 329
>At2g02470.1 68415.m00186 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 256
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 15 SDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQPMPDGLPE 143
++ +V + +T E + LCL PN+ + + + +P LPE
Sbjct: 31 TNDMVKFYQTCDPEKENLCLYGLPNETWEVNLPVEEVPPELPE 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,547,418
Number of Sequences: 28952
Number of extensions: 270322
Number of successful extensions: 721
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -