BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0470.Seq (459 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing... 27 8.1 At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 27 8.1 At1g13470.1 68414.m01578 expressed protein 27 8.1 >At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing enzyme family protein low similarity to cis,cis-muconate lactonizing enzyme [Burkholderia sp. TH2] GI:23491535; contains Pfam profile: PF01188 Mandelate racemase/muconate lactonizing enzyme, C-terminal domain, PF02746: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain Length = 410 Score = 26.6 bits (56), Expect = 8.1 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +3 Query: 282 VVTILRP*NLYLMVGGAFTL*MSMGSSNHLTPG 380 V+ + R + LM+GG ++MG S HL G Sbjct: 328 VIELARSSGIELMIGGMVETRLAMGFSGHLAAG 360 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 26.6 bits (56), Expect = 8.1 Identities = 10/22 (45%), Positives = 17/22 (77%) Frame = +3 Query: 54 RRRVPIFLMKYVLKKCSRLTST 119 ++RVP +M+ VL+ C+RLT + Sbjct: 1564 KQRVPAMIMQAVLQLCARLTKS 1585 >At1g13470.1 68414.m01578 expressed protein Length = 379 Score = 26.6 bits (56), Expect = 8.1 Identities = 14/31 (45%), Positives = 15/31 (48%) Frame = +2 Query: 188 RTSWESARVGTTTPPISAVKQ*SFRFEGWGS 280 R WE R G P AV + S RF G GS Sbjct: 308 RMKWEEERFGWKIEPQRAVVKKSERFNGGGS 338 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,492,576 Number of Sequences: 28952 Number of extensions: 217749 Number of successful extensions: 400 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 398 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 400 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 762235320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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