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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0470.Seq
         (459 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing...    27   8.1  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    27   8.1  
At1g13470.1 68414.m01578 expressed protein                             27   8.1  

>At3g18270.1 68416.m02324 mandelate racemase/muconate lactonizing
           enzyme family protein low similarity to cis,cis-muconate
           lactonizing enzyme [Burkholderia sp. TH2] GI:23491535;
           contains Pfam profile: PF01188 Mandelate
           racemase/muconate lactonizing enzyme, C-terminal domain,
           PF02746: Mandelate racemase/muconate lactonizing enzyme,
           N-terminal domain
          Length = 410

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +3

Query: 282 VVTILRP*NLYLMVGGAFTL*MSMGSSNHLTPG 380
           V+ + R   + LM+GG     ++MG S HL  G
Sbjct: 328 VIELARSSGIELMIGGMVETRLAMGFSGHLAAG 360


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
            identical to ubiquitin-protein ligase 2 [Arabidopsis
            thaliana] GI:7108523; E3, HECT-domain protein family;
            similar to ubiquitin-protein ligase 2 GI:7108523 from
            [Arabidopsis thaliana]
          Length = 3658

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = +3

Query: 54   RRRVPIFLMKYVLKKCSRLTST 119
            ++RVP  +M+ VL+ C+RLT +
Sbjct: 1564 KQRVPAMIMQAVLQLCARLTKS 1585


>At1g13470.1 68414.m01578 expressed protein
          Length = 379

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 14/31 (45%), Positives = 15/31 (48%)
 Frame = +2

Query: 188 RTSWESARVGTTTPPISAVKQ*SFRFEGWGS 280
           R  WE  R G    P  AV + S RF G GS
Sbjct: 308 RMKWEEERFGWKIEPQRAVVKKSERFNGGGS 338


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,492,576
Number of Sequences: 28952
Number of extensions: 217749
Number of successful extensions: 400
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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