Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0465.Seq
(449 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g52600.1 68414.m05938 signal peptidase, putative similar to S... 89 1e-18
At3g15710.1 68416.m01991 signal peptidase, putative similar to S... 85 2e-17
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 27 5.9
At5g40940.1 68418.m04971 hypothetical protein 27 7.7
At5g03100.1 68418.m00258 F-box family protein contains F-box dom... 27 7.7
At3g46640.1 68416.m05063 myb family transcription factor contain... 27 7.7
At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 27 7.7
>At1g52600.1 68414.m05938 signal peptidase, putative similar to
SP|P13679 Microsomal signal peptidase 21 kDa subunit (EC
3.4.-.-) {Canis familiaris}; contains Pfam profile
PF00461: Signal peptidase I
Length = 180
Score = 89.0 bits (211), Expect = 1e-18
Identities = 41/62 (66%), Positives = 49/62 (79%)
Frame = +3
Query: 54 DDVRRMNKRQFMYQVLSFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSMEPAFHRGDLL 233
D ++ + RQ Q +S GMIV+SAL+IWK LM VTGSESP+VVVLSGSMEP F RGD+L
Sbjct: 9 DSIKSIQIRQLFTQAISLGMIVTSALIIWKALMCVTGSESPVVVVLSGSMEPGFKRGDIL 68
Query: 234 FL 239
FL
Sbjct: 69 FL 70
Score = 87.4 bits (207), Expect = 4e-18
Identities = 41/73 (56%), Positives = 52/73 (71%), Gaps = 1/73 (1%)
Frame = +2
Query: 233 VFNQLP*EPVRVGEIVXFKVXGRDIPIVHRVLKLHEK-NNGXVKFLTKGDNNSVDDXXLX 409
+F + +P+R GEIV F V GRDIPIVHRV+K+HE+ N G V LTKGDNN DD L
Sbjct: 68 LFLHMSKDPIRAGEIVVFNVDGRDIPIVHRVIKVHERENTGEVDVLTKGDNNYGDDRLLY 127
Query: 410 AQGQLWLTKKDVV 448
A+GQLWL + ++
Sbjct: 128 AEGQLWLHRHHIM 140
>At3g15710.1 68416.m01991 signal peptidase, putative similar to
SP|P13679 Microsomal signal peptidase 21 kDa subunit (EC
3.4.-.-) {Canis familiaris}; contains Pfam profile:
PF00461 signal peptidase I
Length = 180
Score = 85.0 bits (201), Expect = 2e-17
Identities = 40/78 (51%), Positives = 54/78 (69%), Gaps = 1/78 (1%)
Frame = +2
Query: 218 QRRLAVFNQLP*EPVRVGEIVXFKVXGRDIPIVHRVLKLHEK-NNGXVKFLTKGDNNSVD 394
QR +F ++ EP+R GEIV F V GR+IPIVHR +K+HE+ + V LTKGDNN +D
Sbjct: 63 QRGDILFLRMTDEPIRAGEIVVFSVDGREIPIVHRAIKVHERGDTKAVDVLTKGDNNDID 122
Query: 395 DXXLXAQGQLWLTKKDVV 448
D L A+GQLWL + ++
Sbjct: 123 DIGLYAEGQLWLHRHHIM 140
Score = 83.8 bits (198), Expect = 5e-17
Identities = 38/62 (61%), Positives = 49/62 (79%)
Frame = +3
Query: 54 DDVRRMNKRQFMYQVLSFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSMEPAFHRGDLL 233
D ++ + RQ + Q ++ GMIV+SAL+IWK L+ VTGSESP+VVVLS SMEP F RGD+L
Sbjct: 9 DSIKSIKIRQVLTQAITLGMIVTSALIIWKALICVTGSESPVVVVLSESMEPGFQRGDIL 68
Query: 234 FL 239
FL
Sbjct: 69 FL 70
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
pombe}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 27.1 bits (57), Expect = 5.9
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +2
Query: 311 IVHRVLKLHEKNNGXVKFLTKGDNNSVDD 397
+V R+ KLHE N + K DN++++D
Sbjct: 1139 LVDRLRKLHEGNKKSILSFNKTDNDNLED 1167
>At5g40940.1 68418.m04971 hypothetical protein
Length = 424
Score = 26.6 bits (56), Expect = 7.7
Identities = 19/75 (25%), Positives = 31/75 (41%)
Frame = +3
Query: 27 KLTMLESLFDDVRRMNKRQFMYQVLSFGMIVSSALMIWKGLMVVTGSESPIVVVLSGSME 206
K+T+ + + + + Y + G +VS L++WK L S + VL G
Sbjct: 290 KITVFAPIDEAIPNPTTKFSDYVTIFRGHVVSQ-LLLWKDLQKFAKEGSILQTVLKGYEI 348
Query: 207 PAFHRGDLLFLTNYP 251
GD+L L P
Sbjct: 349 EISLSGDILLLNGVP 363
>At5g03100.1 68418.m00258 F-box family protein contains F-box domain
Pfam:PF00646
Length = 307
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = -2
Query: 196 PDSTTTIGLSLPVTTIKPFHIIKAEDTIIPKLNTW 92
PDS S IK F + + D +P+++TW
Sbjct: 60 PDSLNKTLSSYSTPKIKSFDVTISRDVTVPEIDTW 94
>At3g46640.1 68416.m05063 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 323
Score = 26.6 bits (56), Expect = 7.7
Identities = 10/19 (52%), Positives = 12/19 (63%), Gaps = 3/19 (15%)
Frame = -1
Query: 158 YYHQTF---PYHQGRRHYH 111
Y+HQ PYHQ RH+H
Sbjct: 268 YHHQNHNHDPYHQNHRHHH 286
>At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin
family protein similar to arabinogalactan protein
[Daucus carota] GI:11322245; contains Pfam profile
PF01190: Pollen proteins Ole e I family
Length = 359
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = -1
Query: 140 PYHQGRRHYHTKA*HLVHELPLVHSP 63
PYH G H H H H P H P
Sbjct: 42 PYHHGHHHPHPPHHHHPHPHPHPHPP 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,964,930
Number of Sequences: 28952
Number of extensions: 164816
Number of successful extensions: 377
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 369
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 375
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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