BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0462.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 98 9e-22 SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 96 4e-21 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 28 1.5 SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pom... 27 3.4 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 6.0 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 25 7.9 SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pomb... 25 7.9 SPAC664.08c |||traub transciption factor family protein |Schizos... 25 7.9 >SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 98.3 bits (234), Expect = 9e-22 Identities = 42/86 (48%), Positives = 64/86 (74%) Frame = -1 Query: 512 CVCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCY 333 C+ +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RVF IGFT + + +KT Y Sbjct: 96 CLTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRVFVIGFTRRRANQVKKTTY 155 Query: 332 AQHTQVRAIRKKMCEIITRDVTNSEL 255 AQ +Q+RAIR+KM ++I ++ + Sbjct: 156 AQSSQIRAIRQKMFQVIQNQTSSCSM 181 Score = 63.7 bits (148), Expect = 2e-11 Identities = 27/51 (52%), Positives = 40/51 (78%) Frame = -3 Query: 255 REVVNKLIPDSIAKDIEKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 103 RE+V KLIP+ I ++IE+A I+PL++V +RKVK+LK P+ + KL+ELH Sbjct: 182 RELVQKLIPEVIGREIERATGSIFPLQNVLVRKVKILKAPKHDAQKLLELH 232 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 589 ISKLDTDAERSFRKFRLIAEYVQGRN 512 ++ L D E +FRK +L E +QG++ Sbjct: 70 LADLQKDEEHAFRKVKLRVEDIQGKS 95 >SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 96.3 bits (229), Expect = 4e-21 Identities = 41/77 (53%), Positives = 60/77 (77%) Frame = -1 Query: 512 CVCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCY 333 C+ +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+F IGFT++ +KT Y Sbjct: 96 CLTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRIFVIGFTSRRVNQVKKTTY 155 Query: 332 AQHTQVRAIRKKMCEII 282 AQ +Q+RAI +KM ++I Sbjct: 156 AQSSQIRAIHQKMFQVI 172 Score = 64.5 bits (150), Expect = 1e-11 Identities = 28/51 (54%), Positives = 40/51 (78%) Frame = -3 Query: 255 REVVNKLIPDSIAKDIEKACHGIYPLRDVCIRKVKVLKRPRFEISKLMELH 103 +E+V KLIP+ I + IEKA + IYPL++V +RKVK+LK P+ + KL+ELH Sbjct: 182 KELVQKLIPEVIGRAIEKATNNIYPLQNVFVRKVKILKAPKHDAQKLLELH 232 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = -2 Query: 589 ISKLDTDAERSFRKFRLIAEYVQGRN 512 ++ L D E SFRK +L E +QG++ Sbjct: 70 LADLQKDEEHSFRKVKLRVEDIQGKS 95 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/24 (58%), Positives = 15/24 (62%) Frame = +3 Query: 111 PSTSRSRNGASSTLSPFGCKHRAE 182 PSTSR RN S LS KHRA+ Sbjct: 736 PSTSRERNNNISELSNSRTKHRAK 759 >SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pombe|chr 2|||Manual Length = 464 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +2 Query: 491 CRGSCRHITSLHIFGD*SEFAERPFRVSVKLG 586 C G + + LH+ GD S + F V++K+G Sbjct: 409 CAGPLQKVVGLHLVGDFSAEILQGFGVAIKMG 440 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 25.8 bits (54), Expect = 6.0 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%) Frame = -3 Query: 384 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENV 292 LHW+ L+ +Q + P H+GQ S + NV Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/46 (26%), Positives = 21/46 (45%) Frame = -1 Query: 467 RWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 330 ++ W L+ T GYV+R GFT+ ++ + K +A Sbjct: 795 KFATSNWDKLLSRLPVAGTMRGYVVRFVTSGFTHASAIDKIKEFFA 840 >SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 202 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = -2 Query: 178 ARCLHPKGESVEEAPFRDLEVD 113 +RCL ++V+EA RDLE++ Sbjct: 22 SRCLGFAAQNVDEANLRDLEIE 43 >SPAC664.08c |||traub transciption factor family protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 452 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%) Frame = -1 Query: 488 DLTTD-KLRWMVKKWQTLIEANIDVKTTDGYVLR 390 D T D +RWM K Q + N+D K + G +R Sbjct: 351 DSTRDGTVRWMATKKQKQKKENVDTKASKGRKIR 384 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,873,198 Number of Sequences: 5004 Number of extensions: 59063 Number of successful extensions: 179 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 179 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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