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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0448.Seq
         (598 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g15070.1 68418.m01766 expressed protein                             28   4.1  
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl...    28   4.1  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    28   5.4  
At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon cat...    27   7.2  
At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family prote...    27   7.2  
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat...    27   7.2  
At1g01110.1 68414.m00014 expressed protein contains Prosite PS00...    27   9.5  

>At5g15070.1 68418.m01766 expressed protein
          Length = 1049

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = -1

Query: 463  LLLRSVYLMVRPLVSPREWVPPPC 392
            L L ++  M+RP   P E  PPPC
Sbjct: 992  LTLETMEKMIRPFAMPAEDFPPPC 1015


>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
           identical to ubiquitin-protein ligase 1 [Arabidopsis
           thaliana] GI:7108521; E3, HECT-domain protein family;
           similar to GI:7108521, GB:AAF36454 from [Arabidopsis
           thaliana]
          Length = 3891

 Score = 28.3 bits (60), Expect = 4.1
 Identities = 16/53 (30%), Positives = 24/53 (45%)
 Frame = +3

Query: 3   KFNFFIKNNYKAYVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161
           KF  F  +  K+++N ++ +   L    L  F  E D GD  +W   F  F T
Sbjct: 255 KFMVFEPSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDT 307


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
           identical to ubiquitin-protein ligase 2 [Arabidopsis
           thaliana] GI:7108523; E3, HECT-domain protein family;
           similar to ubiquitin-protein ligase 2 GI:7108523 from
           [Arabidopsis thaliana]
          Length = 3658

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = +3

Query: 39  YVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161
           ++N ++ +   L +  L SF  E D GD  +W   F+ F T
Sbjct: 18  FINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDT 58


>At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon
           catalytic subunit, putative similar to SP|Q07864 DNA
           polymerase epsilon, catalytic subunit A (EC 2.7.7.7)
           (DNA polymerase II subunit A) {Homo sapiens}; contains
           Pfam profiles: PF03175 DNA polymerase type B, organellar
           and viral, PF00136 DNA polymerase family B, PF03104 DNA
           polymerase family B, exonuclease domain
          Length = 2138

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 15/44 (34%), Positives = 24/44 (54%)
 Frame = +3

Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377
           AH C ++   +G++ RK A    + +    +Y G  I+QNA LL
Sbjct: 772 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 814


>At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family protein
           low similarity to SP|Q40708 PIR7A protein {Oryza
           sativa}, polyneuridine aldehyde esterase GI:6651393 from
           [Rauvolfia serpentina], ethylene-induced esterase
           [Citrus sinensis] GI:14279437; contains Pfam profile
           PF00561: hydrolase, alpha/beta fold family
          Length = 444

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 10/28 (35%), Positives = 20/28 (71%)
 Frame = -2

Query: 357 GWGSRCNYETLELISQGGWRIYVVDVHG 274
           G+G+ C Y+T+ L+ + G+++  VD+ G
Sbjct: 195 GFGAWCWYKTITLLEKHGFQVDAVDLTG 222


>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
           catalytic subunit, putative similar to SP|Q07864 DNA
           polymerase epsilon, catalytic subunit A (EC 2.7.7.7)
           (DNA polymerase II subunit A) {Homo sapiens}; contains
           Pfam profiles: PF03175 DNA polymerase type B, organellar
           and viral, PF00136 DNA polymerase family B, PF03104 DNA
           polymerase family B, exonuclease domain
          Length = 2271

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 15/44 (34%), Positives = 24/44 (54%)
 Frame = +3

Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377
           AH C ++   +G++ RK A    + +    +Y G  I+QNA LL
Sbjct: 779 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 821


>At1g01110.1 68414.m00014 expressed protein contains Prosite
           PS00165: Serine/threonine dehydratases
           pyridoxal-phosphate attachment site
          Length = 364

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = +1

Query: 340 TTAAPSFKTHYCFTAEIGRVVVPTRADSQEVLPS 441
           T+  PS +++Y FTA  G  +  T  ++  +LP+
Sbjct: 230 TSNTPSLRSNYSFTARSGCSISTTMVNNASLLPN 263


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,277,287
Number of Sequences: 28952
Number of extensions: 275664
Number of successful extensions: 642
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 642
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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