BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0448.Seq (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g15070.1 68418.m01766 expressed protein 28 4.1 At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 28 4.1 At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 28 5.4 At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon cat... 27 7.2 At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family prote... 27 7.2 At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat... 27 7.2 At1g01110.1 68414.m00014 expressed protein contains Prosite PS00... 27 9.5 >At5g15070.1 68418.m01766 expressed protein Length = 1049 Score = 28.3 bits (60), Expect = 4.1 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -1 Query: 463 LLLRSVYLMVRPLVSPREWVPPPC 392 L L ++ M+RP P E PPPC Sbjct: 992 LTLETMEKMIRPFAMPAEDFPPPC 1015 >At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly identical to ubiquitin-protein ligase 1 [Arabidopsis thaliana] GI:7108521; E3, HECT-domain protein family; similar to GI:7108521, GB:AAF36454 from [Arabidopsis thaliana] Length = 3891 Score = 28.3 bits (60), Expect = 4.1 Identities = 16/53 (30%), Positives = 24/53 (45%) Frame = +3 Query: 3 KFNFFIKNNYKAYVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161 KF F + K+++N ++ + L L F E D GD +W F F T Sbjct: 255 KFMVFEPSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDT 307 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 27.9 bits (59), Expect = 5.4 Identities = 13/41 (31%), Positives = 21/41 (51%) Frame = +3 Query: 39 YVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161 ++N ++ + L + L SF E D GD +W F+ F T Sbjct: 18 FINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDT 58 >At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon catalytic subunit, putative similar to SP|Q07864 DNA polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A) {Homo sapiens}; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain Length = 2138 Score = 27.5 bits (58), Expect = 7.2 Identities = 15/44 (34%), Positives = 24/44 (54%) Frame = +3 Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377 AH C ++ +G++ RK A + + +Y G I+QNA LL Sbjct: 772 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 814 >At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family protein low similarity to SP|Q40708 PIR7A protein {Oryza sativa}, polyneuridine aldehyde esterase GI:6651393 from [Rauvolfia serpentina], ethylene-induced esterase [Citrus sinensis] GI:14279437; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 444 Score = 27.5 bits (58), Expect = 7.2 Identities = 10/28 (35%), Positives = 20/28 (71%) Frame = -2 Query: 357 GWGSRCNYETLELISQGGWRIYVVDVHG 274 G+G+ C Y+T+ L+ + G+++ VD+ G Sbjct: 195 GFGAWCWYKTITLLEKHGFQVDAVDLTG 222 >At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon catalytic subunit, putative similar to SP|Q07864 DNA polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A) {Homo sapiens}; contains Pfam profiles: PF03175 DNA polymerase type B, organellar and viral, PF00136 DNA polymerase family B, PF03104 DNA polymerase family B, exonuclease domain Length = 2271 Score = 27.5 bits (58), Expect = 7.2 Identities = 15/44 (34%), Positives = 24/44 (54%) Frame = +3 Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377 AH C ++ +G++ RK A + + +Y G I+QNA LL Sbjct: 779 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 821 >At1g01110.1 68414.m00014 expressed protein contains Prosite PS00165: Serine/threonine dehydratases pyridoxal-phosphate attachment site Length = 364 Score = 27.1 bits (57), Expect = 9.5 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = +1 Query: 340 TTAAPSFKTHYCFTAEIGRVVVPTRADSQEVLPS 441 T+ PS +++Y FTA G + T ++ +LP+ Sbjct: 230 TSNTPSLRSNYSFTARSGCSISTTMVNNASLLPN 263 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,277,287 Number of Sequences: 28952 Number of extensions: 275664 Number of successful extensions: 642 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 626 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 642 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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