BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0448.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g15070.1 68418.m01766 expressed protein 28 4.1
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 28 4.1
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 28 5.4
At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon cat... 27 7.2
At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family prote... 27 7.2
At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon cat... 27 7.2
At1g01110.1 68414.m00014 expressed protein contains Prosite PS00... 27 9.5
>At5g15070.1 68418.m01766 expressed protein
Length = 1049
Score = 28.3 bits (60), Expect = 4.1
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 463 LLLRSVYLMVRPLVSPREWVPPPC 392
L L ++ M+RP P E PPPC
Sbjct: 992 LTLETMEKMIRPFAMPAEDFPPPC 1015
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +3
Query: 3 KFNFFIKNNYKAYVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161
KF F + K+++N ++ + L L F E D GD +W F F T
Sbjct: 255 KFMVFEPSKIKSFINSVTSVPLELIHEPLACFRWEFDKGDFHHWVDLFNYFDT 307
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 27.9 bits (59), Expect = 5.4
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 39 YVNYMSYIFTLLYESWLESFVGEVD*GDSRYWSQQFFEFST 161
++N ++ + L + L SF E D GD +W F+ F T
Sbjct: 18 FINSVTSVPLELIQEPLASFRWEFDKGDFHHWVDLFYHFDT 58
>At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7)
(DNA polymerase II subunit A) {Homo sapiens}; contains
Pfam profiles: PF03175 DNA polymerase type B, organellar
and viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2138
Score = 27.5 bits (58), Expect = 7.2
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +3
Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377
AH C ++ +G++ RK A + + +Y G I+QNA LL
Sbjct: 772 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 814
>At1g69240.1 68414.m07933 hydrolase, alpha/beta fold family protein
low similarity to SP|Q40708 PIR7A protein {Oryza
sativa}, polyneuridine aldehyde esterase GI:6651393 from
[Rauvolfia serpentina], ethylene-induced esterase
[Citrus sinensis] GI:14279437; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 444
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = -2
Query: 357 GWGSRCNYETLELISQGGWRIYVVDVHG 274
G+G+ C Y+T+ L+ + G+++ VD+ G
Sbjct: 195 GFGAWCWYKTITLLEKHGFQVDAVDLTG 222
>At1g08260.1 68414.m00911 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7)
(DNA polymerase II subunit A) {Homo sapiens}; contains
Pfam profiles: PF03175 DNA polymerase type B, organellar
and viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2271
Score = 27.5 bits (58), Expect = 7.2
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +3
Query: 246 AHLC*VVTGAHGHLQRKCATHLEI*VLRSHSYNGCPILQNALLL 377
AH C ++ +G++ RK A + + +Y G I+QNA LL
Sbjct: 779 AHKC-ILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLL 821
>At1g01110.1 68414.m00014 expressed protein contains Prosite
PS00165: Serine/threonine dehydratases
pyridoxal-phosphate attachment site
Length = 364
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +1
Query: 340 TTAAPSFKTHYCFTAEIGRVVVPTRADSQEVLPS 441
T+ PS +++Y FTA G + T ++ +LP+
Sbjct: 230 TSNTPSLRSNYSFTARSGCSISTTMVNNASLLPN 263
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,277,287
Number of Sequences: 28952
Number of extensions: 275664
Number of successful extensions: 642
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 642
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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