BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0443.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 79 6e-16 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 77 2e-15 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 2.1 SPBC2G2.09c |crs1|mug17|meiosis specific cyclin Crs1|Schizosacch... 27 2.1 SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase G... 25 8.4 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 78.6 bits (185), Expect = 6e-16 Identities = 36/70 (51%), Positives = 42/70 (60%) Frame = +1 Query: 256 VCHTDAYTLSGKDPEGVXPVVLXXXXXXXXXXXXXXXTXVKPGDHVVPLXVXQCNTCKFC 435 VCHTDAYTLSGKDPEG+ PV+L T V+ GD V+ L +C TCKFC Sbjct: 49 VCHTDAYTLSGKDPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFC 108 Query: 436 XNPKTNLARR 465 + KTNL R Sbjct: 109 KSGKTNLCGR 118 Score = 59.7 bits (138), Expect = 3e-10 Identities = 25/45 (55%), Positives = 34/45 (75%) Frame = +3 Query: 462 KVRSTQGQRVMPDGXSDSACXGQELYHFMGXSTFSQYTAVLEISL 596 ++R+TQG+ +MPDG S +C G L HFMG STFS+YT V +IS+ Sbjct: 118 RIRTTQGKGLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISV 162 Score = 55.2 bits (127), Expect = 7e-09 Identities = 23/39 (58%), Positives = 29/39 (74%) Frame = +2 Query: 128 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKI 244 T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKI 44 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 77.0 bits (181), Expect = 2e-15 Identities = 34/70 (48%), Positives = 40/70 (57%) Frame = +1 Query: 256 VCHTDAYTLSGKDPEGVXPVVLXXXXXXXXXXXXXXXTXVKPGDHVVPLXVXQCNTCKFC 435 VCHTDAYTLSG DPEG P+VL V+PGDHV+ L +C CKFC Sbjct: 46 VCHTDAYTLSGVDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFC 105 Query: 436 XNPKTNLARR 465 + KTNL + Sbjct: 106 RSGKTNLCSK 115 Score = 58.4 bits (135), Expect = 7e-10 Identities = 28/41 (68%), Positives = 32/41 (78%) Frame = +2 Query: 122 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKI 244 MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKV 41 Score = 57.6 bits (133), Expect = 1e-09 Identities = 25/45 (55%), Positives = 33/45 (73%) Frame = +3 Query: 462 KVRSTQGQRVMPDGXSDSACXGQELYHFMGXSTFSQYTAVLEISL 596 K+R TQG+ +MPDG S +C + L H+MG S+FSQYT V +ISL Sbjct: 115 KIRETQGRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISL 159 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.1 bits (57), Expect = 2.1 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +2 Query: 533 TLPFYGLXNIQSVHSCSRNF 592 T+PFYGL + HSCS F Sbjct: 552 TVPFYGLESYGLPHSCSTMF 571 >SPBC2G2.09c |crs1|mug17|meiosis specific cyclin Crs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 229 Score = 27.1 bits (57), Expect = 2.1 Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 2/40 (5%) Frame = +3 Query: 18 ICLIGF*YFVTS--SSKRFIARL*FQHFVIGSCPVQSCQQ 131 + L+ F +V S S + F+ L FQH S P +SC Q Sbjct: 175 LALLNFDIYVISLPSVESFLTPLIFQHVFFKSLPSESCDQ 214 >SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase Gde1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1076 Score = 25.0 bits (52), Expect = 8.4 Identities = 22/69 (31%), Positives = 35/69 (50%) Frame = -2 Query: 276 IRVSMADSVAVILTRTSPAFGGSTSISSMDSGLPASHATAAKHLITLPTVDMTALDNCQS 97 IRVS+ AV ++ SP+F S SS+D + S + +K+ T MT S Sbjct: 505 IRVSLQGDDAVYVSGRSPSFAASRP-SSVDF-MSQSTDSLSKNDTTASNGSMTP---SSS 559 Query: 96 QNVEIIVEL 70 QN +I+++ Sbjct: 560 QNNSVIIDI 568 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,125,896 Number of Sequences: 5004 Number of extensions: 36976 Number of successful extensions: 92 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 83 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 91 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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