Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0439.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 77 1e-15
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 71 9e-14
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 51 1e-07
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 36 0.004
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 24 0.34
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 28 1.0
SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|... 27 2.4
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 26 3.2
SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 4.2
SPCC1442.12 |||CDP-diacylglycerol--serine O-phosphatidyltransfer... 26 4.2
SPCC2H8.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 25 5.6
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 25 5.6
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 25 7.3
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 25 7.3
SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 25 7.3
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.3
SPAC26H5.04 |||vacuolar import and degradation protein Vid28|Sch... 25 7.3
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 25 7.3
SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 25 9.7
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 25 9.7
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 9.7
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 25 9.7
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 77.4 bits (182), Expect = 1e-15
Identities = 45/106 (42%), Positives = 60/106 (56%)
Frame = -1
Query: 509 QGRLSKEEIERMVNEAEKYRXEDDKQKETIQAKNALESYLLQHEVYHGG*EAQGKDL*L* 330
+GRLSKEEI+RMV+EAEKY+ ED+ + IQAKN LESY + K
Sbjct: 505 KGRLSKEEIDRMVSEAEKYKAEDEAETSRIQAKNHLESYAYSLRNSLDDPNLKDKVDASD 564
Query: 329 QADHPRQVQRHHQVAGFQPTGRQGGYEHKQKELEGICNPIITKMYQ 192
+ + V+ + T + YE KQKELEG+ NPI+ K+YQ
Sbjct: 565 KEAIDKAVKETIEWLDHNTTAAKDEYEDKQKELEGVANPIMAKIYQ 610
Score = 57.2 bits (132), Expect = 1e-09
Identities = 33/111 (29%), Positives = 56/111 (50%), Gaps = 2/111 (1%)
Frame = -2
Query: 544 TTKETKITITNDKVVSPRKRSSVWLMRQRSTEXRMTSKRRPSR--PRMHWNLTCFSMKST 371
T K KITITNDK + + M + + + + SR + H +S++++
Sbjct: 493 TGKTQKITITNDK--GRLSKEEIDRMVSEAEKYKAEDEAETSRIQAKNHLESYAYSLRNS 550
Query: 370 MEDEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEDMSTSRKNWKAFA 218
++D LK+K+ SDK+ I +TI+WLD N A K++ +K + A
Sbjct: 551 LDDPNLKDKVDASDKEAIDKAVKETIEWLDHNTTAAKDEYEDKQKELEGVA 601
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 71.3 bits (167), Expect = 9e-14
Identities = 43/106 (40%), Positives = 58/106 (54%)
Frame = -1
Query: 509 QGRLSKEEIERMVNEAEKYRXEDDKQKETIQAKNALESYLLQHEVYHGG*EAQGKDL*L* 330
+GRLSKEEI+RMV EAEKY+ ED+ + IQAKN LESY + K
Sbjct: 505 KGRLSKEEIDRMVAEAEKYKAEDEAESGRIQAKNHLESYAYSLRNSLDDPNLKDKVDASD 564
Query: 329 QADHPRQVQRHHQVAGFQPTGRQGGYEHKQKELEGICNPIITKMYQ 192
+ + V+ + T + +E KQKELE + NPI+ K+YQ
Sbjct: 565 KETVDKAVKETIEWLDSNTTAAKDEFEAKQKELESVANPIMAKIYQ 610
Score = 61.3 bits (142), Expect = 9e-11
Identities = 31/109 (28%), Positives = 57/109 (52%)
Frame = -2
Query: 544 TTKETKITITNDKVVSPRKRSSVWLMRQRSTEXRMTSKRRPSRPRMHWNLTCFSMKSTME 365
T K KITITNDK ++ + + ++ + + H +S++++++
Sbjct: 493 TGKTQKITITNDKGRLSKEEIDRMVAEAEKYKAEDEAESGRIQAKNHLESYAYSLRNSLD 552
Query: 364 DEKLKEKISDSDKQTILDKCNDTIKWLDSNQLADKEDMSTSRKNWKAFA 218
D LK+K+ SDK+T+ +TI+WLDSN A K++ +K ++ A
Sbjct: 553 DPNLKDKVDASDKETVDKAVKETIEWLDSNTTAAKDEFEAKQKELESVA 601
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 51.2 bits (117), Expect = 1e-07
Identities = 36/112 (32%), Positives = 59/112 (52%), Gaps = 7/112 (6%)
Frame = -1
Query: 509 QGRLSKEEIERMVNEAEKYRXEDDKQKETIQAKNALESYLL-------QHEVYHGG*EAQ 351
+GRLS+E+IERMV EAE++ ED KE I+A+N LE+Y E G + +
Sbjct: 535 KGRLSEEDIERMVKEAEEFAEEDKILKERIEARNTLENYAYSLKGQFDDDEQLGGKVDPE 594
Query: 350 GKDL*L*QADHPRQVQRHHQVAGFQPTGRQGGYEHKQKELEGICNPIITKMY 195
K L D V ++ G + + +E ++++L+ + +PI K+Y
Sbjct: 595 DKQAVL---DAVEDVAEWLEIHGEDASKEE--FEDQRQKLDAVVHPITQKLY 641
Score = 36.7 bits (81), Expect = 0.002
Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 2/57 (3%)
Frame = -2
Query: 388 FSMKSTMED-EKLKEKISDSDKQTILDKCNDTIKWLD-SNQLADKEDMSTSRKNWKA 224
+S+K +D E+L K+ DKQ +LD D +WL+ + A KE+ R+ A
Sbjct: 575 YSLKGQFDDDEQLGGKVDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFEDQRQKLDA 631
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 35.9 bits (79), Expect = 0.004
Identities = 18/35 (51%), Positives = 22/35 (62%)
Frame = -1
Query: 500 LSKEEIERMVNEAEKYRXEDDKQKETIQAKNALES 396
L+ EIE MV +AEKYR D +KE I+ N ES
Sbjct: 550 LTDSEIEAMVADAEKYRASDMARKEAIENGNRAES 584
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 24.2 bits (50), Expect(2) = 0.34
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -2
Query: 343 ISDSDKQTILDKCNDTIKWLDSNQLADK 260
I +K T+ D+ NDT +WLD +L DK
Sbjct: 215 IGKDEKYTLYDQ-NDTKEWLD--KLGDK 239
Score = 23.8 bits (49), Expect(2) = 0.34
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 193 RVPEESPEVCRASRAEHPEPEVP 125
++ ++ P RASRA EP+ P
Sbjct: 235 KLGDKGPAAARASRAAAEEPQAP 257
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 27.9 bits (59), Expect = 1.0
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = -1
Query: 512 RQGRLSKEEIERMVNEAEKYRXEDDKQ-KETIQAKNALESYL 390
++ RL E +E+ EAE DK ET+ KNALE Y+
Sbjct: 547 QEDRLPTEVLEKY-REAEHQMIATDKLVAETVDRKNALEEYI 587
>SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 440
Score = 26.6 bits (56), Expect = 2.4
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -1
Query: 296 HQVAGFQPTGRQGGYEHKQKEL 231
H + GFQP+ RQ Y+H +L
Sbjct: 290 HHLNGFQPSQRQEDYKHTMVDL 311
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 3.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 184 EESPEVCRASRAEHPEPE 131
EE+P AS EHPEP+
Sbjct: 244 EEAPAAAAASENEHPEPK 261
>SPBP18G5.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 430
Score = 25.8 bits (54), Expect = 4.2
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = -2
Query: 547 STTKETKITITNDKVVSPRKRSSVWLMRQRSTEXRMTSKRRPSRPRMH 404
S KE K TI + + SP++ S + +++RR S+P+ H
Sbjct: 189 SLEKEVK-TIYDSNIASPKESSDAIDADHAMIDESRSTQRRKSKPKKH 235
>SPCC1442.12 |||CDP-diacylglycerol--serine O-phosphatidyltransferase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 250
Score = 25.8 bits (54), Expect = 4.2
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +2
Query: 362 ILHGRLHAEASKIPMHSWPGWSPFACHP 445
IL G+ H +P W G +PFA HP
Sbjct: 194 ILKGKTH---ENLPWGEWCGNTPFAFHP 218
>SPCC2H8.04 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 266
Score = 25.4 bits (53), Expect = 5.6
Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
Frame = -2
Query: 487 RSSVWLMRQRST---EXRMTSKRRPSRPRMHWNLTCFSMKSTMEDEKLKEKISDSD 329
R W QR + + R+T K ++ R+ +++C + +EDE +K IS+ D
Sbjct: 149 RPKFWPQSQRISGMLKKRITMKE--NKHRLIQSMSCMLRHAKLEDEFMKAYISEED 202
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 25.4 bits (53), Expect = 5.6
Identities = 12/28 (42%), Positives = 15/28 (53%), Gaps = 1/28 (3%)
Frame = +2
Query: 173 RGLLRHPGTSSLLSDCKCLPILSA-CAH 253
R ++ H GT + D CLP SA C H
Sbjct: 122 REVVHHEGTCEICYDEGCLPFFSAECDH 149
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 25.0 bits (52), Expect = 7.3
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = -1
Query: 512 RQGRLSKEEIERMVNEAEKYRXEDDKQKETIQAKNALE 399
R+ RL +EE +R++ E K R + DK++E + + LE
Sbjct: 689 REQRLREEEEKRILEE-RKRREKLDKEEEERRRRELLE 725
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 25.0 bits (52), Expect = 7.3
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -1
Query: 509 QGRLSKEEIERMVNEAEK 456
+G LSKEE+ER V +A K
Sbjct: 525 KGTLSKEELERRVYDAAK 542
>SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 25.0 bits (52), Expect = 7.3
Identities = 7/29 (24%), Positives = 18/29 (62%)
Frame = -2
Query: 370 MEDEKLKEKISDSDKQTILDKCNDTIKWL 284
+++++++ K+S D L+ CN+ + L
Sbjct: 260 LKEQEIRRKVSSDDVHNYLESCNNHLSML 288
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.0 bits (52), Expect = 7.3
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = -2
Query: 145 HPEPEVPPPGLEALAPPSRRSIKPTFHXTRKPTCNNHLVTSP 20
H +P +L+PPS R++KP + T ++ +SP
Sbjct: 169 HLRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC26H5.04 |||vacuolar import and degradation protein
Vid28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 729
Score = 25.0 bits (52), Expect = 7.3
Identities = 13/36 (36%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = -2
Query: 529 KITITNDKVV-SPRKRSSVWLMRQRSTEXRMTSKRR 425
++ + N KV SPR S+++ + R+T+ R+ S+RR
Sbjct: 176 RLILYNSKVGDSPRFCSALFQITNRATQLRLYSRRR 211
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.0 bits (52), Expect = 7.3
Identities = 15/38 (39%), Positives = 17/38 (44%), Gaps = 2/38 (5%)
Frame = -2
Query: 190 VPEESPEVCRA--SRAEHPEPEVPPPGLEALAPPSRRS 83
V +SP R SR P E P A PPSRR+
Sbjct: 891 VSRDSPRYSRGGYSRGSVPPRETLAPSKGAYVPPSRRN 928
>SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog
Rhp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 963
Score = 24.6 bits (51), Expect = 9.7
Identities = 18/59 (30%), Positives = 25/59 (42%)
Frame = -2
Query: 499 SPRKRSSVWLMRQRSTEXRMTSKRRPSRPRMHWNLTCFSMKSTMEDEKLKEKISDSDKQ 323
S R+R S S+ R +K PS R H+ L + EKL+E+ KQ
Sbjct: 288 SKRRRVSARASSSASSSSRTQAKSIPSHERTHYRLIRQHPELEHVWEKLEEEAPREVKQ 346
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 24.6 bits (51), Expect = 9.7
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = -1
Query: 515 QRQGRLSKEEIERMVNEAEKYRXEDDKQKETIQAK-NALES--YLLQHEV 375
Q +L++E +ER+ E ++YR + + E K +LE +LQ E+
Sbjct: 946 QAASKLAEEMVERVKTEYDEYRLQTSESLEKNHLKITSLEQRIVILQDEI 995
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 24.6 bits (51), Expect = 9.7
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = -1
Query: 539 QGDQDHHYQRQGRLSKEEIERMVNEAEKYRXEDDKQKETIQAKNA 405
Q + D +R G + EEI ++++ E E+D++ E N+
Sbjct: 606 QDESDILSERIGAPTAEEISQLLDSVETNEEENDEELEKAMGVNS 650
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 24.6 bits (51), Expect = 9.7
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 385 SMKSTMEDEKLKEKISD 335
S+K MEDEKL EK+++
Sbjct: 114 SLKLIMEDEKLIEKVNE 130
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,965,177
Number of Sequences: 5004
Number of extensions: 38402
Number of successful extensions: 199
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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