BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0436.Seq
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g21950.1 68418.m02549 hydrolase, alpha/beta fold family prote... 31 0.87
At5g09430.1 68418.m01092 hydrolase, alpha/beta fold family prote... 28 6.1
>At5g21950.1 68418.m02549 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas fluorescens] GI:1871461, hydrolase EtbD2
[Rhodococcus sp.] GI:3273241; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 300
Score = 30.7 bits (66), Expect = 0.87
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = +2
Query: 44 AVVFSNLMKRLGYKKYYVQGGDWGAFIGSSIATIFPNEV 160
A+ LM++L +++ V G +G F+ ++A +FP +V
Sbjct: 108 ALCMGKLMEKLEVERFSVVGTSYGGFVAYNMAKMFPEKV 146
>At5g09430.1 68418.m01092 hydrolase, alpha/beta fold family protein
low similarity to hydrolases from Rhodococcus sp. EtbD1
GI:3273239, EtbD2 GI:3273241; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 303
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +2
Query: 62 LMKRLGYKKYYVQGGDWGAFIGSSIATIFPNEV 160
LM+ G ++ + G +G F+G S+A FP V
Sbjct: 120 LMEAHGVQRMNIVGISYGGFVGYSLAAQFPENV 152
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,718,893
Number of Sequences: 28952
Number of extensions: 316915
Number of successful extensions: 643
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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