Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0424.Seq
(400 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14770.2 68414.m01766 expressed protein 31 0.28
At1g14770.1 68414.m01765 expressed protein 31 0.28
At1g23280.1 68414.m02912 MAK16 protein-related contains similari... 30 0.49
At2g22795.1 68415.m02704 expressed protein 29 0.86
At5g20680.1 68418.m02456 expressed protein predicted proteins, A... 29 1.5
At3g53670.1 68416.m05927 expressed protein 28 2.0
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 28 2.6
At3g01100.1 68416.m00015 early-responsive to dehydration protein... 27 3.5
At2g04865.1 68415.m00502 expressed protein ; expression supporte... 27 3.5
At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:100... 27 3.5
At3g50050.1 68416.m05472 aspartyl protease family protein contai... 27 4.6
At2g27790.1 68415.m03369 expressed protein 27 4.6
At5g58130.1 68418.m07273 RNA recognition motif (RRM)-containing ... 27 6.1
At5g15700.1 68418.m01836 DNA-directed RNA polymerase (RPOT2) ide... 27 6.1
At4g28270.1 68417.m04049 zinc finger (C3HC4-type RING finger) fa... 27 6.1
At4g02320.1 68417.m00316 pectinesterase family protein contains ... 27 6.1
At1g69670.1 68414.m08018 cullin, putative contains similarity to... 27 6.1
At5g27160.1 68418.m03241 hypothetical protein contains Pfam prof... 26 8.0
At5g02390.1 68418.m00162 expressed protein ; expression supporte... 26 8.0
At4g07520.1 68417.m01174 hypothetical protein contains Pfam prof... 26 8.0
At4g02250.1 68417.m00304 invertase/pectin methylesterase inhibit... 26 8.0
At3g23750.1 68416.m02986 leucine-rich repeat family protein / pr... 26 8.0
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu... 26 8.0
At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containi... 26 8.0
>At1g14770.2 68414.m01766 expressed protein
Length = 429
Score = 31.1 bits (67), Expect = 0.28
Identities = 17/46 (36%), Positives = 24/46 (52%)
Frame = -2
Query: 399 EANFSVGKNEKIDSDEQMIGIAANLQATEKDEVKTGDKESDKIETS 262
E + +G NEK DEQ + I L EKD+V D E + + T+
Sbjct: 206 EQHVHIG-NEKESIDEQQVHIGLELNRNEKDKVIAIDDEDEPMHTN 250
>At1g14770.1 68414.m01765 expressed protein
Length = 429
Score = 31.1 bits (67), Expect = 0.28
Identities = 17/46 (36%), Positives = 24/46 (52%)
Frame = -2
Query: 399 EANFSVGKNEKIDSDEQMIGIAANLQATEKDEVKTGDKESDKIETS 262
E + +G NEK DEQ + I L EKD+V D E + + T+
Sbjct: 206 EQHVHIG-NEKESIDEQQVHIGLELNRNEKDKVIAIDDEDEPMHTN 250
>At1g23280.1 68414.m02912 MAK16 protein-related contains similarity
to MAK16 protein SP:P10962 from [Saccharomyces
cerevisiae]
Length = 303
Score = 30.3 bits (65), Expect = 0.49
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -1
Query: 226 EVKTDDKKSNKIETSGIIENVEREETIEPELSDTLSD 116
E++T+D+ + E G+IE VE ++ +E E + + D
Sbjct: 193 EIETNDEVEKEEEEEGVIEYVEGDDELEAEEEEDMED 229
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 29.5 bits (63), Expect = 0.86
Identities = 17/41 (41%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Frame = -1
Query: 244 KREDTYEVKTDDKKSNKIETSGIIENVERE-ETIEPELSDT 125
K E + + K +DK++ KIE+S + E E+E ET E E S +
Sbjct: 476 KVESSSQEKNEDKETEKIESSFLEETKEKEDETKEKEESSS 516
Score = 28.7 bits (61), Expect = 1.5
Identities = 22/66 (33%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
Frame = -2
Query: 372 EKIDSDEQMIGIAANLQATEKDEV----KTGDKESDKIETSGIERM*NVKTHTKLKQTIK 205
EK +S Q + +A EK E K DKE++KIE+S +E + TK K+
Sbjct: 456 EKEESSSQEETMDKETEAKEKVESSSQEKNEDKETEKIESSFLEETKEKEDETKEKEESS 515
Query: 204 SLIKSK 187
S K++
Sbjct: 516 SQEKTE 521
>At5g20680.1 68418.m02456 expressed protein predicted proteins,
Arabidopsis thaliana
Length = 551
Score = 28.7 bits (61), Expect = 1.5
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -1
Query: 163 EREETIEPELSDTLSDAVPINVVDPITNNHINLKPDNFWINKY 35
E T+ S TL D P+N+ DP T + ++L ++ +Y
Sbjct: 337 ETNTTVLYHWSSTLCDIEPLNITDPATEHAMHLDRPPAFLRQY 379
>At3g53670.1 68416.m05927 expressed protein
Length = 203
Score = 28.3 bits (60), Expect = 2.0
Identities = 12/44 (27%), Positives = 26/44 (59%)
Frame = -1
Query: 223 VKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVD 92
V +DD + + + SG I EE+ + +S+ + +++P+N +D
Sbjct: 34 VGSDDAQESDGDDSGYIHQTVIEESKDKAISEPIPESLPLNSLD 77
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 27.9 bits (59), Expect = 2.6
Identities = 13/45 (28%), Positives = 26/45 (57%)
Frame = -1
Query: 217 TDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVDPIT 83
+DD+ K +++ ++ E ++PE+ D LSD + + V+ IT
Sbjct: 395 SDDRILGKRSIHELLQQIDPSEKLDPEVEDILSD-IAEDFVESIT 438
>At3g01100.1 68416.m00015 early-responsive to dehydration
protein-related / ERD protein-related low similarity to
ERD4 protein (early-responsive to dehydration stress)
[Arabidopsis thaliana] GI:15375406; contains Pfam
profile PF02714: Domain of unknown function DUF221
Length = 703
Score = 27.5 bits (58), Expect = 3.5
Identities = 23/81 (28%), Positives = 35/81 (43%), Gaps = 8/81 (9%)
Frame = +2
Query: 125 CIGQFRF-NSLFTFYIL-YNTGS------FDFIRLFIVCFNFVCVFTFHILSIPEVSILS 280
C+G + N L Y Y TG + +V + + V F + +P S L+
Sbjct: 574 CLGYIIYRNQLLNVYAAKYETGGKFWPIVHSYTIFSLVLMHIIAVGLFGLKELPVASSLT 633
Query: 281 DSLSPVLTSSFSVACKLAAMP 343
L PVLT FS+ C+ +P
Sbjct: 634 IPL-PVLTVLFSIYCQRRFLP 653
>At2g04865.1 68415.m00502 expressed protein ; expression supported
by MPSS
Length = 667
Score = 27.5 bits (58), Expect = 3.5
Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = -1
Query: 250 NVKREDTY-EVKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDA 113
+VK+ED +V+ DD + SG EN REE E E+ ++++++
Sbjct: 609 DVKKEDKESKVEDDDAAKGFSDVSGE-ENANREEEDETEMGESVAES 654
>At1g28400.1 68414.m03492 expressed protein similar to E6
(GI:1000090) [Gossypium barbadense]
Length = 335
Score = 27.5 bits (58), Expect = 3.5
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -1
Query: 274 NRNFWY*ENVKREDTYEVKTDDKKSNKIETSGIIENVERE 155
N N+ Y ENVK E+++ +D K N ++ +ERE
Sbjct: 189 NYNYKYDENVK-EESFPENNEDNKKNVYNSNAYGTELERE 227
>At3g50050.1 68416.m05472 aspartyl protease family protein contains
Pfam PF00026: Eukaryotic aspartyl protease
Length = 632
Score = 27.1 bits (57), Expect = 4.6
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 4/58 (6%)
Frame = -1
Query: 211 DKKSNKIETSGII----ENVEREETIEPELSDTLSDAVPINVVDPITNNHINLKPDNF 50
D KS+++ S + E++ R + PE S S+ N+V TN+ I L P+ F
Sbjct: 507 DVKSSQVSLSNLTSKGNESLVRMVVLPPEPSTWFSNVTATNIVSRFTNHQIKL-PEIF 563
>At2g27790.1 68415.m03369 expressed protein
Length = 240
Score = 27.1 bits (57), Expect = 4.6
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -1
Query: 223 VKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVDP 89
VKT +K NKIE SG + ++ +PE S P + + P
Sbjct: 183 VKTVREKLNKIEESGNQKRLQPSSQTQPEESGNQKRLQPSSQIQP 227
>At5g58130.1 68418.m07273 RNA recognition motif (RRM)-containing
protein
Length = 748
Score = 26.6 bits (56), Expect = 6.1
Identities = 18/73 (24%), Positives = 32/73 (43%), Gaps = 4/73 (5%)
Frame = -1
Query: 247 VKREDTYEVKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPI----NVVDPITN 80
V+ + + DD SN + S +NVE + I+ DT +D V ++ D +++
Sbjct: 471 VENDTAIDSMADDTVSNSMAESDDGDNVEDDTAIDSMCDDTANDDVGSDDSGSLADTVSD 530
Query: 79 NHINLKPDNFWIN 41
+ P F N
Sbjct: 531 TSVEAVPLEFVAN 543
>At5g15700.1 68418.m01836 DNA-directed RNA polymerase (RPOT2)
identical to phage-type RNA polymerase rpoT2
[Arabidopsis thaliana] GI:11340683
Length = 1011
Score = 26.6 bits (56), Expect = 6.1
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = -2
Query: 309 DEVKTGDKESDKIETSGIERM*NVKTHTKLKQTIKSLIKSK 187
D+ K GD D E+ G+E ++K KL++ + LIK +
Sbjct: 303 DKKKKGD---DNEESGGVENETSMKEQDKLRKKVNELIKKQ 340
>At4g28270.1 68417.m04049 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 193
Score = 26.6 bits (56), Expect = 6.1
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +2
Query: 185 SFDFIRLFIVCFNFVCVFTF 244
S + LF++CF F+C+F F
Sbjct: 174 SLSRVYLFLLCFMFMCLFLF 193
>At4g02320.1 68417.m00316 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 518
Score = 26.6 bits (56), Expect = 6.1
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +3
Query: 177 IPEVSILLDFLSSVLTSYVSSRFTFS 254
I ++ LL FLS+V +S+V+ RFT S
Sbjct: 9 ILSLTFLLLFLSTVFSSHVTQRFTSS 34
>At1g69670.1 68414.m08018 cullin, putative contains similarity to
Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from
[Homo sapiens]; contains Pfam profile PF00888: Cullin
family
Length = 732
Score = 26.6 bits (56), Expect = 6.1
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +2
Query: 86 DRVYNINRNSVR*CIGQFRFNSLFTFYILYNTGSFDFIRLFI 211
DR+ N+ N+ + Q NS F +++ NT S +FI LF+
Sbjct: 344 DRIINMAFNNDK--TFQNALNSSFEYFVNLNTRSPEFISLFV 383
>At5g27160.1 68418.m03241 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 702
Score = 26.2 bits (55), Expect = 8.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -2
Query: 336 AANLQATEKDEVKTGDKESDKIETSGIE 253
A E D V+TG E+ +ET G+E
Sbjct: 535 AVETDGVETDGVETGGVETHDVETDGVE 562
>At5g02390.1 68418.m00162 expressed protein ; expression supported
by MPSS
Length = 835
Score = 26.2 bits (55), Expect = 8.0
Identities = 15/42 (35%), Positives = 28/42 (66%)
Frame = -2
Query: 315 EKDEVKTGDKESDKIETSGIERM*NVKTHTKLKQTIKSLIKS 190
EK + K +E K + + ER + KTH+++K+++K+LIK+
Sbjct: 105 EKTKKKHNPEEKSK-KLNSEER--SRKTHSEIKRSVKALIKA 143
>At4g07520.1 68417.m01174 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 734
Score = 26.2 bits (55), Expect = 8.0
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = -2
Query: 336 AANLQATEKDEVKTGDKESDKIETSGIE 253
A E D V+TG E+ +ET G+E
Sbjct: 590 AVETDGVETDGVETGGVETHDVETDGVE 617
>At4g02250.1 68417.m00304 invertase/pectin methylesterase inhibitor
family protein low similarity to SP|P83326
Pectinesterase inhibitor (Pectin methylesterase
inhibitor) (PMEI) {Actinidia chinensis}, pistil-specific
gene sts15 [Solanum tuberosum] GI:1616628; contains Pfam
profile PF04043: Plant invertase/pectin methylesterase
inhibitor
Length = 145
Score = 26.2 bits (55), Expect = 8.0
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -1
Query: 202 SNKIETSGIIENVEREETIEPELSDTLSD 116
SN +TS I+ +++ +EP L DTL D
Sbjct: 39 SNASDTSFYIKAKLKQKNLEPALEDTLDD 67
>At3g23750.1 68416.m02986 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 928
Score = 26.2 bits (55), Expect = 8.0
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2
Query: 20 GIISCVFVYPEIIGFKVYMVVSDRVY 97
G+I V V+ I+GF VY V R Y
Sbjct: 478 GVIVAVLVFLAILGFVVYKFVMKRKY 503
>At1g79280.1 68414.m09242 expressed protein weak similarity to
Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
Length = 2111
Score = 26.2 bits (55), Expect = 8.0
Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 5/55 (9%)
Frame = -2
Query: 336 AANLQATEKDE----VKTGDKESDKIETSGIERM*NVKTHTK-LKQTIKSLIKSK 187
A +L + EK+ + +G +SD +E SG++R+ + TK + +T SL++ +
Sbjct: 1191 AKHLNSAEKNSRSGTISSGSTDSDHLEDSGLQRVVHYLRRTKEIAETEISLMRQE 1245
>At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containing
protein low similarity to SP|P46825 Kinesin light chain
(KLC) {Loligo pealeii}; contains Pfam profile PF00515:
TPR Domain
Length = 1797
Score = 26.2 bits (55), Expect = 8.0
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = -2
Query: 318 TEKDEVKTGDKESDKIETSGIERM*NVKTHTKLKQTIKS 202
TEKD+ +TG+K ++++ G+ + +K K + S
Sbjct: 568 TEKDKKQTGEKSKNELKVEGLGKPLKSLNSSKKKTDVSS 606
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,137,962
Number of Sequences: 28952
Number of extensions: 121238
Number of successful extensions: 540
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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