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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0422.Seq
         (400 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g07020.1 68415.m00803 protein kinase family protein contains ...    30   0.49 
At4g04910.1 68417.m00714 AAA-type ATPase family protein similar ...    30   0.65 
At3g05660.1 68416.m00630 disease resistance family protein conta...    29   1.5  
At4g38560.1 68417.m05459 expressed protein                             28   2.6  
At1g14270.1 68414.m01692 CAAX amino terminal protease family pro...    27   4.6  
At5g49310.1 68418.m06102 importin alpha-1 subunit, putative simi...    26   8.0  
At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph...    26   8.0  

>At2g07020.1 68415.m00803 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 700

 Score = 30.3 bits (65), Expect = 0.49
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = -2

Query: 261 LSNSSKLTTSDARPSVDWF*SNKSTHPITGQSSD 160
           +S+S     S  RPS+DWF  N+S +  +  SS+
Sbjct: 221 VSDSDLSFVSSDRPSMDWFEDNRSNYATSSSSSE 254


>At4g04910.1 68417.m00714 AAA-type ATPase family protein similar to
           SP|P18708 Vesicular-fusion protein NSF
           (N-ethylmaleimide-sensitive fusion protein)
           (NEM-sensitive fusion protein) {Cricetulus griseus};
           contains Pfam profiles PF00004: ATPase AAA family,
           PF02359: Cell division protein 48 (CDC48) N-terminal
           domain; contains non-consensus AT-AC splice sites at
           intron 2
          Length = 742

 Score = 29.9 bits (64), Expect = 0.65
 Identities = 15/29 (51%), Positives = 20/29 (68%), Gaps = 2/29 (6%)
 Frame = +2

Query: 200 DQNQSTEGLASE--VVNFDELDNFCRSHG 280
           +Q+Q T G ASE  V+ FDE+D  C+S G
Sbjct: 307 EQDQRTLGDASELHVIIFDEIDAICKSRG 335


>At3g05660.1 68416.m00630 disease resistance family protein contains
           leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
           pimpinellifolium] gi|1184077|gb|AAC15780
          Length = 883

 Score = 28.7 bits (61), Expect = 1.5
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = +2

Query: 188 VLLLDQNQSTEGLASEVVNFDELDNFCRSHGQVPATHLSNV 310
           +L LD N+ +  L  EV+N  +L     SH Q   T   N+
Sbjct: 211 ILRLDNNKLSGNLPLEVINLTKLSEISLSHNQFTGTLPPNI 251


>At4g38560.1 68417.m05459 expressed protein
          Length = 521

 Score = 27.9 bits (59), Expect = 2.6
 Identities = 15/48 (31%), Positives = 25/48 (52%)
 Frame = +1

Query: 7   SYMLVSKIKPCMSQCKPY*GDTANGSIYQFWFLRSYSVTWITVVILEL 150
           SY + + +   + +     GD A+GS  Q    +SYS+  +  V+LEL
Sbjct: 341 SYKVRASVSSTLQKILDKHGDIASGSKLQSLRTKSYSLETLAAVVLEL 388


>At1g14270.1 68414.m01692 CAAX amino terminal protease family
           protein contains Pfam profile PF02517: CAAX amino
           terminal protease family
          Length = 353

 Score = 27.1 bits (57), Expect = 4.6
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = +2

Query: 5   VVICLSQRLSHACLSASRIKAIPRMAQY 88
           ++ CLSQ  S  CLS SR   +P+   Y
Sbjct: 18  IISCLSQSSSLLCLSDSRRLILPKTCTY 45


>At5g49310.1 68418.m06102 importin alpha-1 subunit, putative similar
           to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 519

 Score = 26.2 bits (55), Expect = 8.0
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = -3

Query: 227 RGPPSIGFDLIKALIPSLVRVL 162
           RG PS  FDL+K ++P L R++
Sbjct: 228 RGKPSPPFDLVKHVLPVLKRLV 249


>At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) /
           phosphoglyceride transfer protein similar to
           polyphosphoinositide binding protein SEC14 homolog Ssh1p
           (GB:AAB94598) [Glycine max]; identified in Eur J Biochem
           1998 Dec 1;258(2):402-10 as AtSEC14, characterized by
           functional complementation in S. cerevisiae.
          Length = 325

 Score = 26.2 bits (55), Expect = 8.0
 Identities = 8/25 (32%), Positives = 16/25 (64%)
 Frame = +2

Query: 233 EVVNFDELDNFCRSHGQVPATHLSN 307
           ++++++ L +FCR  G     H+SN
Sbjct: 231 KIMDYESLPHFCRREGSGSGRHISN 255


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,608,417
Number of Sequences: 28952
Number of extensions: 159636
Number of successful extensions: 359
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 359
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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