BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0422.Seq
(400 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g07020.1 68415.m00803 protein kinase family protein contains ... 30 0.49
At4g04910.1 68417.m00714 AAA-type ATPase family protein similar ... 30 0.65
At3g05660.1 68416.m00630 disease resistance family protein conta... 29 1.5
At4g38560.1 68417.m05459 expressed protein 28 2.6
At1g14270.1 68414.m01692 CAAX amino terminal protease family pro... 27 4.6
At5g49310.1 68418.m06102 importin alpha-1 subunit, putative simi... 26 8.0
At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph... 26 8.0
>At2g07020.1 68415.m00803 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 700
Score = 30.3 bits (65), Expect = 0.49
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = -2
Query: 261 LSNSSKLTTSDARPSVDWF*SNKSTHPITGQSSD 160
+S+S S RPS+DWF N+S + + SS+
Sbjct: 221 VSDSDLSFVSSDRPSMDWFEDNRSNYATSSSSSE 254
>At4g04910.1 68417.m00714 AAA-type ATPase family protein similar to
SP|P18708 Vesicular-fusion protein NSF
(N-ethylmaleimide-sensitive fusion protein)
(NEM-sensitive fusion protein) {Cricetulus griseus};
contains Pfam profiles PF00004: ATPase AAA family,
PF02359: Cell division protein 48 (CDC48) N-terminal
domain; contains non-consensus AT-AC splice sites at
intron 2
Length = 742
Score = 29.9 bits (64), Expect = 0.65
Identities = 15/29 (51%), Positives = 20/29 (68%), Gaps = 2/29 (6%)
Frame = +2
Query: 200 DQNQSTEGLASE--VVNFDELDNFCRSHG 280
+Q+Q T G ASE V+ FDE+D C+S G
Sbjct: 307 EQDQRTLGDASELHVIIFDEIDAICKSRG 335
>At3g05660.1 68416.m00630 disease resistance family protein contains
leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 883
Score = 28.7 bits (61), Expect = 1.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +2
Query: 188 VLLLDQNQSTEGLASEVVNFDELDNFCRSHGQVPATHLSNV 310
+L LD N+ + L EV+N +L SH Q T N+
Sbjct: 211 ILRLDNNKLSGNLPLEVINLTKLSEISLSHNQFTGTLPPNI 251
>At4g38560.1 68417.m05459 expressed protein
Length = 521
Score = 27.9 bits (59), Expect = 2.6
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +1
Query: 7 SYMLVSKIKPCMSQCKPY*GDTANGSIYQFWFLRSYSVTWITVVILEL 150
SY + + + + + GD A+GS Q +SYS+ + V+LEL
Sbjct: 341 SYKVRASVSSTLQKILDKHGDIASGSKLQSLRTKSYSLETLAAVVLEL 388
>At1g14270.1 68414.m01692 CAAX amino terminal protease family
protein contains Pfam profile PF02517: CAAX amino
terminal protease family
Length = 353
Score = 27.1 bits (57), Expect = 4.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 5 VVICLSQRLSHACLSASRIKAIPRMAQY 88
++ CLSQ S CLS SR +P+ Y
Sbjct: 18 IISCLSQSSSLLCLSDSRRLILPKTCTY 45
>At5g49310.1 68418.m06102 importin alpha-1 subunit, putative similar
to importin alpha-1 subunit (Karyopherin alpha-1
subunit, KAP alpha) [Arabidopsis thaliana]
SWISS-PROT:Q96321
Length = 519
Score = 26.2 bits (55), Expect = 8.0
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -3
Query: 227 RGPPSIGFDLIKALIPSLVRVL 162
RG PS FDL+K ++P L R++
Sbjct: 228 RGKPSPPFDLVKHVLPVLKRLV 249
>At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) /
phosphoglyceride transfer protein similar to
polyphosphoinositide binding protein SEC14 homolog Ssh1p
(GB:AAB94598) [Glycine max]; identified in Eur J Biochem
1998 Dec 1;258(2):402-10 as AtSEC14, characterized by
functional complementation in S. cerevisiae.
Length = 325
Score = 26.2 bits (55), Expect = 8.0
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = +2
Query: 233 EVVNFDELDNFCRSHGQVPATHLSN 307
++++++ L +FCR G H+SN
Sbjct: 231 KIMDYESLPHFCRREGSGSGRHISN 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,608,417
Number of Sequences: 28952
Number of extensions: 159636
Number of successful extensions: 359
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 359
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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