BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0400.Seq (406 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 99 1e-22 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 99 1e-22 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 50 1e-07 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 99 bits (238), Expect = 1e-22 Identities = 44/50 (88%), Positives = 47/50 (94%) Frame = -3 Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKDYEEVGMDS 105 RLDHKFDLMY+KRAFVHW VGEGM EGEFSEAREDL+ALE+DYEEVG DS Sbjct: 394 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443 Score = 91.1 bits (216), Expect = 6e-20 Identities = 39/52 (75%), Positives = 44/52 (84%) Frame = -1 Query: 403 TIQFVDWCPTGFKVGINYQPPTVVPXGDLAKVQRAVCMLSNTTAIAEAWAAL 248 TIQFVDWCPTGFK+GI Y+PP VP +AKV RAVCMLSNTT+IAEAW+ L Sbjct: 344 TIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRL 395 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 99 bits (238), Expect = 1e-22 Identities = 44/50 (88%), Positives = 47/50 (94%) Frame = -3 Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKDYEEVGMDS 105 RLDHKFDLMY+KRAFVHW VGEGM EGEFSEAREDL+ALE+DYEEVG DS Sbjct: 390 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439 Score = 84.2 bits (199), Expect = 7e-18 Identities = 36/52 (69%), Positives = 43/52 (82%) Frame = -1 Query: 403 TIQFVDWCPTGFKVGINYQPPTVVPXGDLAKVQRAVCMLSNTTAIAEAWAAL 248 TIQFVDWCPTGFK+GI +PP + ++AKV RAVCMLSNTT+IAEAW+ L Sbjct: 340 TIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRL 391 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 50.4 bits (115), Expect = 1e-07 Identities = 22/52 (42%), Positives = 34/52 (65%), Gaps = 3/52 (5%) Frame = -3 Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKD---YEEVGMD 108 RL +F M+ ++AF+HW GEGM E EF+EA +++ L + Y+E G+D Sbjct: 380 RLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,472,416 Number of Sequences: 5004 Number of extensions: 23232 Number of successful extensions: 72 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 70 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 72 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 138190552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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