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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0400.Seq
         (406 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    99   1e-22
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    99   1e-22
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    50   1e-07

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =   99 bits (238), Expect = 1e-22
 Identities = 44/50 (88%), Positives = 47/50 (94%)
 Frame = -3

Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKDYEEVGMDS 105
           RLDHKFDLMY+KRAFVHW VGEGM EGEFSEAREDL+ALE+DYEEVG DS
Sbjct: 394 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443



 Score = 91.1 bits (216), Expect = 6e-20
 Identities = 39/52 (75%), Positives = 44/52 (84%)
 Frame = -1

Query: 403 TIQFVDWCPTGFKVGINYQPPTVVPXGDLAKVQRAVCMLSNTTAIAEAWAAL 248
           TIQFVDWCPTGFK+GI Y+PP  VP   +AKV RAVCMLSNTT+IAEAW+ L
Sbjct: 344 TIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRL 395


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =   99 bits (238), Expect = 1e-22
 Identities = 44/50 (88%), Positives = 47/50 (94%)
 Frame = -3

Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKDYEEVGMDS 105
           RLDHKFDLMY+KRAFVHW VGEGM EGEFSEAREDL+ALE+DYEEVG DS
Sbjct: 390 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439



 Score = 84.2 bits (199), Expect = 7e-18
 Identities = 36/52 (69%), Positives = 43/52 (82%)
 Frame = -1

Query: 403 TIQFVDWCPTGFKVGINYQPPTVVPXGDLAKVQRAVCMLSNTTAIAEAWAAL 248
           TIQFVDWCPTGFK+GI  +PP  +   ++AKV RAVCMLSNTT+IAEAW+ L
Sbjct: 340 TIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRL 391


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 50.4 bits (115), Expect = 1e-07
 Identities = 22/52 (42%), Positives = 34/52 (65%), Gaps = 3/52 (5%)
 Frame = -3

Query: 254 RLDHKFDLMYAKRAFVHWXVGEGMXEGEFSEAREDLSALEKD---YEEVGMD 108
           RL  +F  M+ ++AF+HW  GEGM E EF+EA  +++ L  +   Y+E G+D
Sbjct: 380 RLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,472,416
Number of Sequences: 5004
Number of extensions: 23232
Number of successful extensions: 72
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 72
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 138190552
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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