BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0386.Seq (403 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 42 5e-05 SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||M... 29 0.36 SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce... 25 3.3 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 25 3.3 SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosacchar... 24 7.7 SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 24 7.7 >SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 41.5 bits (93), Expect = 5e-05 Identities = 18/34 (52%), Positives = 22/34 (64%) Frame = +2 Query: 152 KFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKT 253 K+ ID T D I DV FEKYL + +KV+GKT Sbjct: 11 KYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKT 44 >SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 28.7 bits (61), Expect = 0.36 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = -1 Query: 136 LDAFAADFATLHSFFASLLVQLGFLS 59 +D + DFAT H+ + + L +GFLS Sbjct: 87 IDLYILDFATQHNLYVASLRNMGFLS 112 >SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 25.4 bits (53), Expect = 3.3 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +3 Query: 267 SRCRRQG*DESRYHRRHSFFKEVPEVFNKTLPQEEQF 377 SR R + + ++ R SF +VPE + TLP+ F Sbjct: 135 SRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDF 171 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 25.4 bits (53), Expect = 3.3 Identities = 12/44 (27%), Positives = 24/44 (54%), Gaps = 1/44 (2%) Frame = -2 Query: 219 YFSKLPTSKILSSAGCVQSIVNFKLIFR-LMPLPRILPPFTPFL 91 ++ + ++ G +++I ++F L L RI+ PFTPF+ Sbjct: 729 WYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFI 772 >SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 24.2 bits (50), Expect = 7.7 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = -1 Query: 82 LVQLGFLSHRFLCYCHCGKRTRVEQ 8 +++ G LSHR+ C KRT E+ Sbjct: 61 MIRAGRLSHRYKGIGECFKRTAAEE 85 >SPBC4B4.04 |||translation initiation factor eIF2A |Schizosaccharomyces pombe|chr 2|||Manual Length = 576 Score = 24.2 bits (50), Expect = 7.7 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -2 Query: 333 PL*KRNVCGDSDFRLIPGDDNVIA 262 P+ K V G SDF L PG ++ +A Sbjct: 159 PIAKLRVEGISDFALSPGQNHAVA 182 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,504,813 Number of Sequences: 5004 Number of extensions: 26336 Number of successful extensions: 67 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 67 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 67 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 136158338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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