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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0386.Seq
         (403 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa...    42   5e-05
SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||M...    29   0.36 
SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyce...    25   3.3  
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|...    25   3.3  
SPBC530.10c |anc1||adenine nucleotide carrier Anc1|Schizosacchar...    24   7.7  
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar...    24   7.7  

>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
           L22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 41.5 bits (93), Expect = 5e-05
 Identities = 18/34 (52%), Positives = 22/34 (64%)
 Frame = +2

Query: 152 KFTIDCTHPAEDSILDVGNFEKYLKEHVKVEGKT 253
           K+ ID T    D I DV  FEKYL + +KV+GKT
Sbjct: 11  KYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKT 44


>SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 172

 Score = 28.7 bits (61), Expect = 0.36
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = -1

Query: 136 LDAFAADFATLHSFFASLLVQLGFLS 59
           +D +  DFAT H+ + + L  +GFLS
Sbjct: 87  IDLYILDFATQHNLYVASLRNMGFLS 112


>SPBC409.04c |mis12||kinetochore protein Mis12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = +3

Query: 267 SRCRRQG*DESRYHRRHSFFKEVPEVFNKTLPQEEQF 377
           SR R +  +  ++  R SF  +VPE +  TLP+   F
Sbjct: 135 SRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDF 171


>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1064

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 12/44 (27%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
 Frame = -2

Query: 219 YFSKLPTSKILSSAGCVQSIVNFKLIFR-LMPLPRILPPFTPFL 91
           ++ +    ++    G +++I    ++F  L  L RI+ PFTPF+
Sbjct: 729 WYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFI 772


>SPBC530.10c |anc1||adenine nucleotide carrier
           Anc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 322

 Score = 24.2 bits (50), Expect = 7.7
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = -1

Query: 82  LVQLGFLSHRFLCYCHCGKRTRVEQ 8
           +++ G LSHR+     C KRT  E+
Sbjct: 61  MIRAGRLSHRYKGIGECFKRTAAEE 85


>SPBC4B4.04 |||translation initiation factor eIF2A
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 576

 Score = 24.2 bits (50), Expect = 7.7
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = -2

Query: 333 PL*KRNVCGDSDFRLIPGDDNVIA 262
           P+ K  V G SDF L PG ++ +A
Sbjct: 159 PIAKLRVEGISDFALSPGQNHAVA 182


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,504,813
Number of Sequences: 5004
Number of extensions: 26336
Number of successful extensions: 67
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 67
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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