BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0367.Seq (299 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g53130.1 68414.m06016 stigma-specific Stig1 family protein si... 33 0.047 At5g14920.1 68418.m01750 gibberellin-regulated family protein si... 32 0.082 At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family prote... 29 0.76 At1g77000.1 68414.m08967 F-box family protein similar to GP|2155... 28 1.0 At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 27 1.8 At1g69280.1 68414.m07943 expressed protein 27 1.8 At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containi... 26 4.1 At4g18820.1 68417.m02778 expressed protein 26 4.1 At3g04980.1 68416.m00541 DNAJ heat shock N-terminal domain-conta... 26 4.1 At1g21410.1 68414.m02679 F-box family protein similar to SKP1 i... 26 4.1 At5g59960.1 68418.m07520 expressed protein 26 5.4 At5g10550.1 68418.m01221 DNA-binding bromodomain-containing prot... 25 7.1 At5g08730.1 68418.m01037 IBR domain-containing protein contains ... 25 7.1 At2g22990.5 68415.m02735 sinapoylglucose:malate sinapoyltransfer... 25 7.1 At2g22990.4 68415.m02733 sinapoylglucose:malate sinapoyltransfer... 25 7.1 At2g22990.3 68415.m02736 sinapoylglucose:malate sinapoyltransfer... 25 7.1 At2g22990.2 68415.m02737 sinapoylglucose:malate sinapoyltransfer... 25 7.1 At2g22990.1 68415.m02734 sinapoylglucose:malate sinapoyltransfer... 25 7.1 At1g50720.1 68414.m05703 stigma-specific Stig1 family protein si... 25 9.4 >At1g53130.1 68414.m06016 stigma-specific Stig1 family protein similar to stigma-specific protein STIG1 [Nicotiana tabacum] GI:496647; contains Pfam profile PF04885: Stigma-specific protein, Stig1 Length = 168 Score = 32.7 bits (71), Expect = 0.047 Identities = 19/63 (30%), Positives = 29/63 (46%) Frame = -2 Query: 190 LNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHHCWSCI 11 L+ C +CR ++ ++C C HKC + Q RC C C ++ +HC C Sbjct: 102 LHCCKKHCRNVLGDRNNCGRCGHKCGFGQ-----RC-----CGGVCTYVNFNPNHCGKCT 151 Query: 10 HKC 2 KC Sbjct: 152 RKC 154 >At5g14920.1 68418.m01750 gibberellin-regulated family protein similar to SP|P46689 Gibberellin-regulated protein 1 precursor {Arabidopsis thaliana}; contains Pfam profile PF02704: Gibberellin regulated protein Length = 275 Score = 31.9 bits (69), Expect = 0.082 Identities = 24/87 (27%), Positives = 28/87 (32%), Gaps = 1/87 (1%) Frame = +2 Query: 20 PTVVMEVYHPPAVPVTLIQ-TQTAKDPEHLAVAALMDTVPTVVMEVYHPPAVPVTLIQTQ 196 P V PP P T T K P T P V Y+PP PV Sbjct: 141 PPTTPPVQSPPVQPPTYKPPTSPVKPPTTTPPVKPPTTTPPVQPPTYNPPTTPVKPPTAP 200 Query: 197 TAQDPVHLAVAALMDTVPTA*WKCIIH 277 + P V +D VP +C H Sbjct: 201 PVKPPTPPPVRTRIDCVPLCGTRCGQH 227 Score = 26.2 bits (55), Expect = 4.1 Identities = 14/41 (34%), Positives = 16/41 (39%) Frame = +2 Query: 14 TAPTVVMEVYHPPAVPVTLIQTQTAKDPEHLAVAALMDTVP 136 T P V Y+PP PV K P V +D VP Sbjct: 178 TTPPVQPPTYNPPTTPVKPPTAPPVKPPTPPPVRTRIDCVP 218 Score = 25.4 bits (53), Expect = 7.1 Identities = 15/48 (31%), Positives = 19/48 (39%), Gaps = 1/48 (2%) Frame = +2 Query: 41 YHPPAVPVTLIQTQTAKDPEHLAVAALMDTVPTV-VMEVYHPPAVPVT 181 Y PP +P T I+ T K P + P V + PP P T Sbjct: 59 YKPPTLPTTPIKPPTTKPPVKPPTIPVTPVKPPVSTPPIKLPPVQPPT 106 >At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family protein low similarity to SP|Q02104 Lipase 1 precursor (EC 3.1.1.3) (Triacylglycerol lipase) {Psychrobacter immobilis}, SP|P27747|ACOC_ALCEU Dihydrolipoamide acetyltransferase component of acetoin cleaving system (EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes eutrophus}; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 417 Score = 28.7 bits (61), Expect = 0.76 Identities = 10/44 (22%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Frame = -2 Query: 244 CIHKCCYRQMY-WILR-CLSLNQCYWNCRWMI-HFHHDCWNCIH 122 C C + +++ W+++ C+ + +W + + H HH W+ +H Sbjct: 292 CFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMH 335 >At1g77000.1 68414.m08967 F-box family protein similar to GP|21554029| F-box protein AtFBL5 from [Arabidopsis thaliana]; similar to F-box protein FBL2 GI:6063090 from [Homo sapiens] Length = 360 Score = 28.3 bits (60), Expect = 1.0 Identities = 15/59 (25%), Positives = 25/59 (42%) Frame = -2 Query: 205 LRCLSLNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHH 29 LR L+++QC + + D + +H C R + CL+L + C H H Sbjct: 294 LRSLNISQCTYLTPSAVQAVCDTFPALHTCSGRHSLVMSGCLNLQSVHCACILQAHRTH 352 >At3g61670.1 68416.m06911 expressed protein weak similarity to extra-large G-protein [Arabidopsis thaliana] GI:3201682 Length = 790 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/20 (45%), Positives = 11/20 (55%) Frame = -2 Query: 154 HFHHDCWNCIHKCCYRQMFW 95 HFHH +C H CY +W Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339 >At1g69280.1 68414.m07943 expressed protein Length = 400 Score = 27.5 bits (58), Expect = 1.8 Identities = 18/58 (31%), Positives = 24/58 (41%), Gaps = 7/58 (12%) Frame = -2 Query: 265 LPSRCWNCIHKCCYRQMYWILRCLSLNQCYW-------NCRWMIHFHHDCWNCIHKCC 113 LPS +N CC R + C + C W +C W+ H CW+C CC Sbjct: 317 LPSCGYNFF--CCKR----LKCCPCFSWCRWPSCDYNSSCGWLFCCHWSCWSCC--CC 366 Score = 26.2 bits (55), Expect = 4.1 Identities = 17/64 (26%), Positives = 24/64 (37%), Gaps = 12/64 (18%) Frame = -2 Query: 169 CRWMIHFHHD-CWNCIH-KCCYRQMFWILR---CLSLNQCYW-------NCRWMIHFHHH 26 C W+ + C++C C F R C + C W +C W+ H Sbjct: 300 CSWLCCKNTGPCFSCCRLPSCGYNFFCCKRLKCCPCFSWCRWPSCDYNSSCGWLFCCHWS 359 Query: 25 CWSC 14 CWSC Sbjct: 360 CWSC 363 >At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 904 Score = 26.2 bits (55), Expect = 4.1 Identities = 10/31 (32%), Positives = 17/31 (54%), Gaps = 1/31 (3%) Frame = -2 Query: 190 LNQCYWNCRWM-IHFHHDCWNCIHKCCYRQM 101 L Y+ R IH++ + +NC+ CC R + Sbjct: 648 LQHLYYRIRKSGIHWNQEMYNCVINCCARAL 678 >At4g18820.1 68417.m02778 expressed protein Length = 1111 Score = 26.2 bits (55), Expect = 4.1 Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 4/33 (12%) Frame = -2 Query: 271 DTLPSRCWNCIHK----CCYRQMYWILRCLSLN 185 DTL S CWN + K R + +IL C SL+ Sbjct: 555 DTLSSDCWNALSKVVDRAAPRHVVFILVCSSLD 587 >At3g04980.1 68416.m00541 DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226 DnaJ domain Length = 1165 Score = 26.2 bits (55), Expect = 4.1 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = -2 Query: 100 FWILRCLSLNQCYWNCRWMIHFHHHCWSC 14 FW RC QCY R ++ HC SC Sbjct: 147 FWT-RCRHCGQCYKYLREYMNTSMHCSSC 174 >At1g21410.1 68414.m02679 F-box family protein similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250 Length = 360 Score = 26.2 bits (55), Expect = 4.1 Identities = 15/60 (25%), Positives = 25/60 (41%) Frame = -2 Query: 205 LRCLSLNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHH 26 LR L+++QC + D + +H C R + CL+L + C H H+ Sbjct: 294 LRSLNISQCTALTPSAVQAVCDSFPALHTCSGRHSLVMSGCLNLTTVHCACILQAHRAHN 353 >At5g59960.1 68418.m07520 expressed protein Length = 359 Score = 25.8 bits (54), Expect = 5.4 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = -2 Query: 262 PSRCWNCIHKCCYRQMYWILRCL 194 PS CWN + C +Y I+R + Sbjct: 327 PSSCWNFVVSCIKAVLYSIVRLI 349 >At5g10550.1 68418.m01221 DNA-binding bromodomain-containing protein low similarity to kinase [Gallus gallus] GI:1370092; contains Pfam profile PF00439: Bromodomain Length = 678 Score = 25.4 bits (53), Expect = 7.1 Identities = 9/31 (29%), Positives = 19/31 (61%) Frame = +2 Query: 89 KDPEHLAVAALMDTVPTVVMEVYHPPAVPVT 181 K PE +++A +D+V ++ + PP + +T Sbjct: 388 KGPEQISIAKKLDSVKPLLPTLPPPPVIEIT 418 >At5g08730.1 68418.m01037 IBR domain-containing protein contains similarity to Swiss-Prot:Q94981 ariadne-1 protein (Ari-1) [Drosophila melanogaster] Length = 500 Score = 25.4 bits (53), Expect = 7.1 Identities = 9/29 (31%), Positives = 12/29 (41%) Frame = -2 Query: 214 YWILRCLSLNQCYWNCRWMIHFHHDCWNC 128 Y ++ C+ N W C H WNC Sbjct: 256 YRLINCICSNNFCWICLRTEEQHQGNWNC 284 >At2g22990.5 68415.m02735 sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa]; contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltransferase (SNG1) GI:8699618 Length = 433 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%) Frame = -2 Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83 +CL L + Y C I+ HH DC N CY + ++ C Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299 >At2g22990.4 68415.m02733 sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa]; contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltransferase (SNG1) GI:8699618 Length = 416 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%) Frame = -2 Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83 +CL L + Y C I+ HH DC N CY + ++ C Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299 >At2g22990.3 68415.m02736 sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa]; contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltransferase (SNG1) GI:8699618 Length = 458 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%) Frame = -2 Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83 +CL L + Y C I+ HH DC N CY + ++ C Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299 >At2g22990.2 68415.m02737 sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa]; contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltransferase (SNG1) GI:8699618 Length = 319 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%) Frame = -2 Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83 +CL L + Y C I+ HH DC N CY + ++ C Sbjct: 140 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 185 >At2g22990.1 68415.m02734 sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP:P37890) [Oryza sativa]; contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltransferase (SNG1) GI:8699618 Length = 433 Score = 25.4 bits (53), Expect = 7.1 Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%) Frame = -2 Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83 +CL L + Y C I+ HH DC N CY + ++ C Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299 >At1g50720.1 68414.m05703 stigma-specific Stig1 family protein similar to stigma-specific protein STIG1 [Nicotiana tabacum] GI:496647; contains Pfam profile PF04885: Stigma-specific protein, Stig1 Length = 154 Score = 25.0 bits (52), Expect = 9.4 Identities = 14/60 (23%), Positives = 24/60 (40%) Frame = -2 Query: 181 CYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHHCWSCIHKC 2 C C + + + +C C ++C + Q C C C ++ + HC C H C Sbjct: 91 CSNKCVDLAYDNDNCGACKNQCKFTQT-----C-----CRGECVYLAYDKRHCGECNHSC 140 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 5,800,172 Number of Sequences: 28952 Number of extensions: 109087 Number of successful extensions: 285 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 223 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 279 length of database: 12,070,560 effective HSP length: 70 effective length of database: 10,043,920 effective search space used: 291273680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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