BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0367.Seq
(299 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53130.1 68414.m06016 stigma-specific Stig1 family protein si... 33 0.047
At5g14920.1 68418.m01750 gibberellin-regulated family protein si... 32 0.082
At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family prote... 29 0.76
At1g77000.1 68414.m08967 F-box family protein similar to GP|2155... 28 1.0
At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 27 1.8
At1g69280.1 68414.m07943 expressed protein 27 1.8
At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containi... 26 4.1
At4g18820.1 68417.m02778 expressed protein 26 4.1
At3g04980.1 68416.m00541 DNAJ heat shock N-terminal domain-conta... 26 4.1
At1g21410.1 68414.m02679 F-box family protein similar to SKP1 i... 26 4.1
At5g59960.1 68418.m07520 expressed protein 26 5.4
At5g10550.1 68418.m01221 DNA-binding bromodomain-containing prot... 25 7.1
At5g08730.1 68418.m01037 IBR domain-containing protein contains ... 25 7.1
At2g22990.5 68415.m02735 sinapoylglucose:malate sinapoyltransfer... 25 7.1
At2g22990.4 68415.m02733 sinapoylglucose:malate sinapoyltransfer... 25 7.1
At2g22990.3 68415.m02736 sinapoylglucose:malate sinapoyltransfer... 25 7.1
At2g22990.2 68415.m02737 sinapoylglucose:malate sinapoyltransfer... 25 7.1
At2g22990.1 68415.m02734 sinapoylglucose:malate sinapoyltransfer... 25 7.1
At1g50720.1 68414.m05703 stigma-specific Stig1 family protein si... 25 9.4
>At1g53130.1 68414.m06016 stigma-specific Stig1 family protein
similar to stigma-specific protein STIG1 [Nicotiana
tabacum] GI:496647; contains Pfam profile PF04885:
Stigma-specific protein, Stig1
Length = 168
Score = 32.7 bits (71), Expect = 0.047
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = -2
Query: 190 LNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHHCWSCI 11
L+ C +CR ++ ++C C HKC + Q RC C C ++ +HC C
Sbjct: 102 LHCCKKHCRNVLGDRNNCGRCGHKCGFGQ-----RC-----CGGVCTYVNFNPNHCGKCT 151
Query: 10 HKC 2
KC
Sbjct: 152 RKC 154
>At5g14920.1 68418.m01750 gibberellin-regulated family protein
similar to SP|P46689 Gibberellin-regulated protein 1
precursor {Arabidopsis thaliana}; contains Pfam profile
PF02704: Gibberellin regulated protein
Length = 275
Score = 31.9 bits (69), Expect = 0.082
Identities = 24/87 (27%), Positives = 28/87 (32%), Gaps = 1/87 (1%)
Frame = +2
Query: 20 PTVVMEVYHPPAVPVTLIQ-TQTAKDPEHLAVAALMDTVPTVVMEVYHPPAVPVTLIQTQ 196
P V PP P T T K P T P V Y+PP PV
Sbjct: 141 PPTTPPVQSPPVQPPTYKPPTSPVKPPTTTPPVKPPTTTPPVQPPTYNPPTTPVKPPTAP 200
Query: 197 TAQDPVHLAVAALMDTVPTA*WKCIIH 277
+ P V +D VP +C H
Sbjct: 201 PVKPPTPPPVRTRIDCVPLCGTRCGQH 227
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/41 (34%), Positives = 16/41 (39%)
Frame = +2
Query: 14 TAPTVVMEVYHPPAVPVTLIQTQTAKDPEHLAVAALMDTVP 136
T P V Y+PP PV K P V +D VP
Sbjct: 178 TTPPVQPPTYNPPTTPVKPPTAPPVKPPTPPPVRTRIDCVP 218
Score = 25.4 bits (53), Expect = 7.1
Identities = 15/48 (31%), Positives = 19/48 (39%), Gaps = 1/48 (2%)
Frame = +2
Query: 41 YHPPAVPVTLIQTQTAKDPEHLAVAALMDTVPTV-VMEVYHPPAVPVT 181
Y PP +P T I+ T K P + P V + PP P T
Sbjct: 59 YKPPTLPTTPIKPPTTKPPVKPPTIPVTPVKPPVSTPPIKLPPVQPPT 106
>At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family protein
low similarity to SP|Q02104 Lipase 1 precursor (EC
3.1.1.3) (Triacylglycerol lipase) {Psychrobacter
immobilis}, SP|P27747|ACOC_ALCEU Dihydrolipoamide
acetyltransferase component of acetoin cleaving system
(EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes
eutrophus}; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 417
Score = 28.7 bits (61), Expect = 0.76
Identities = 10/44 (22%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = -2
Query: 244 CIHKCCYRQMY-WILR-CLSLNQCYWNCRWMI-HFHHDCWNCIH 122
C C + +++ W+++ C+ + +W + + H HH W+ +H
Sbjct: 292 CFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMH 335
>At1g77000.1 68414.m08967 F-box family protein similar to
GP|21554029| F-box protein AtFBL5 from [Arabidopsis
thaliana]; similar to F-box protein FBL2 GI:6063090 from
[Homo sapiens]
Length = 360
Score = 28.3 bits (60), Expect = 1.0
Identities = 15/59 (25%), Positives = 25/59 (42%)
Frame = -2
Query: 205 LRCLSLNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHH 29
LR L+++QC + + D + +H C R + CL+L + C H H
Sbjct: 294 LRSLNISQCTYLTPSAVQAVCDTFPALHTCSGRHSLVMSGCLNLQSVHCACILQAHRTH 352
>At3g61670.1 68416.m06911 expressed protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 790
Score = 27.5 bits (58), Expect = 1.8
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = -2
Query: 154 HFHHDCWNCIHKCCYRQMFW 95
HFHH +C H CY +W
Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339
>At1g69280.1 68414.m07943 expressed protein
Length = 400
Score = 27.5 bits (58), Expect = 1.8
Identities = 18/58 (31%), Positives = 24/58 (41%), Gaps = 7/58 (12%)
Frame = -2
Query: 265 LPSRCWNCIHKCCYRQMYWILRCLSLNQCYW-------NCRWMIHFHHDCWNCIHKCC 113
LPS +N CC R + C + C W +C W+ H CW+C CC
Sbjct: 317 LPSCGYNFF--CCKR----LKCCPCFSWCRWPSCDYNSSCGWLFCCHWSCWSCC--CC 366
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/64 (26%), Positives = 24/64 (37%), Gaps = 12/64 (18%)
Frame = -2
Query: 169 CRWMIHFHHD-CWNCIH-KCCYRQMFWILR---CLSLNQCYW-------NCRWMIHFHHH 26
C W+ + C++C C F R C + C W +C W+ H
Sbjct: 300 CSWLCCKNTGPCFSCCRLPSCGYNFFCCKRLKCCPCFSWCRWPSCDYNSSCGWLFCCHWS 359
Query: 25 CWSC 14
CWSC
Sbjct: 360 CWSC 363
>At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 904
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/31 (32%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = -2
Query: 190 LNQCYWNCRWM-IHFHHDCWNCIHKCCYRQM 101
L Y+ R IH++ + +NC+ CC R +
Sbjct: 648 LQHLYYRIRKSGIHWNQEMYNCVINCCARAL 678
>At4g18820.1 68417.m02778 expressed protein
Length = 1111
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 4/33 (12%)
Frame = -2
Query: 271 DTLPSRCWNCIHK----CCYRQMYWILRCLSLN 185
DTL S CWN + K R + +IL C SL+
Sbjct: 555 DTLSSDCWNALSKVVDRAAPRHVVFILVCSSLD 587
>At3g04980.1 68416.m00541 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile PF00226
DnaJ domain
Length = 1165
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -2
Query: 100 FWILRCLSLNQCYWNCRWMIHFHHHCWSC 14
FW RC QCY R ++ HC SC
Sbjct: 147 FWT-RCRHCGQCYKYLREYMNTSMHCSSC 174
>At1g21410.1 68414.m02679 F-box family protein similar to SKP1
interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 360
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/60 (25%), Positives = 25/60 (41%)
Frame = -2
Query: 205 LRCLSLNQCYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHH 26
LR L+++QC + D + +H C R + CL+L + C H H+
Sbjct: 294 LRSLNISQCTALTPSAVQAVCDSFPALHTCSGRHSLVMSGCLNLTTVHCACILQAHRAHN 353
>At5g59960.1 68418.m07520 expressed protein
Length = 359
Score = 25.8 bits (54), Expect = 5.4
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = -2
Query: 262 PSRCWNCIHKCCYRQMYWILRCL 194
PS CWN + C +Y I+R +
Sbjct: 327 PSSCWNFVVSCIKAVLYSIVRLI 349
>At5g10550.1 68418.m01221 DNA-binding bromodomain-containing protein
low similarity to kinase [Gallus gallus] GI:1370092;
contains Pfam profile PF00439: Bromodomain
Length = 678
Score = 25.4 bits (53), Expect = 7.1
Identities = 9/31 (29%), Positives = 19/31 (61%)
Frame = +2
Query: 89 KDPEHLAVAALMDTVPTVVMEVYHPPAVPVT 181
K PE +++A +D+V ++ + PP + +T
Sbjct: 388 KGPEQISIAKKLDSVKPLLPTLPPPPVIEIT 418
>At5g08730.1 68418.m01037 IBR domain-containing protein contains
similarity to Swiss-Prot:Q94981 ariadne-1 protein
(Ari-1) [Drosophila melanogaster]
Length = 500
Score = 25.4 bits (53), Expect = 7.1
Identities = 9/29 (31%), Positives = 12/29 (41%)
Frame = -2
Query: 214 YWILRCLSLNQCYWNCRWMIHFHHDCWNC 128
Y ++ C+ N W C H WNC
Sbjct: 256 YRLINCICSNNFCWICLRTEEQHQGNWNC 284
>At2g22990.5 68415.m02735 sinapoylglucose:malate sinapoyltransferase
(SNG1) similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:malate sinapoyltransferase (SNG1)
GI:8699618
Length = 433
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%)
Frame = -2
Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83
+CL L + Y C I+ HH DC N CY + ++ C
Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299
>At2g22990.4 68415.m02733 sinapoylglucose:malate sinapoyltransferase
(SNG1) similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:malate sinapoyltransferase (SNG1)
GI:8699618
Length = 416
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%)
Frame = -2
Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83
+CL L + Y C I+ HH DC N CY + ++ C
Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299
>At2g22990.3 68415.m02736 sinapoylglucose:malate sinapoyltransferase
(SNG1) similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:malate sinapoyltransferase (SNG1)
GI:8699618
Length = 458
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%)
Frame = -2
Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83
+CL L + Y C I+ HH DC N CY + ++ C
Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299
>At2g22990.2 68415.m02737 sinapoylglucose:malate sinapoyltransferase
(SNG1) similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:malate sinapoyltransferase (SNG1)
GI:8699618
Length = 319
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%)
Frame = -2
Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83
+CL L + Y C I+ HH DC N CY + ++ C
Sbjct: 140 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 185
>At2g22990.1 68415.m02734 sinapoylglucose:malate sinapoyltransferase
(SNG1) similar to serine carboxypeptidase I precursor
(SP:P37890) [Oryza sativa]; contains Pfam profile
PF00450: Serine carboxypeptidase; identical to cDNA
sinapoylglucose:malate sinapoyltransferase (SNG1)
GI:8699618
Length = 433
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/46 (30%), Positives = 20/46 (43%), Gaps = 6/46 (13%)
Frame = -2
Query: 202 RCLSLNQCYWNCRWMIHFHH----DC--WNCIHKCCYRQMFWILRC 83
+CL L + Y C I+ HH DC N CY + ++ C
Sbjct: 254 QCLKLTEEYHKCTAKINIHHILTPDCDVTNVTSPDCYYYPYHLIEC 299
>At1g50720.1 68414.m05703 stigma-specific Stig1 family protein
similar to stigma-specific protein STIG1 [Nicotiana
tabacum] GI:496647; contains Pfam profile PF04885:
Stigma-specific protein, Stig1
Length = 154
Score = 25.0 bits (52), Expect = 9.4
Identities = 14/60 (23%), Positives = 24/60 (40%)
Frame = -2
Query: 181 CYWNCRWMIHFHHDCWNCIHKCCYRQMFWILRCLSLNQCYWNCRWMIHFHHHCWSCIHKC 2
C C + + + +C C ++C + Q C C C ++ + HC C H C
Sbjct: 91 CSNKCVDLAYDNDNCGACKNQCKFTQT-----C-----CRGECVYLAYDKRHCGECNHSC 140
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,800,172
Number of Sequences: 28952
Number of extensions: 109087
Number of successful extensions: 285
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 279
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 291273680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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