BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0359.Seq (548 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g02580.1 68415.m00198 cytochrome P450 family protein 31 0.38 At5g15860.2 68418.m01856 expressed protein 29 2.0 At5g15860.1 68418.m01855 expressed protein 29 2.0 At1g76590.1 68414.m08912 zinc-binding family protein similar to ... 29 2.7 At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SD... 28 3.6 At3g10630.1 68416.m01278 glycosyl transferase family 1 protein c... 28 3.6 At1g62440.1 68414.m07044 leucine-rich repeat family protein / ex... 28 3.6 At1g35940.1 68414.m04463 AT hook motif-containing protein-relate... 28 3.6 At2g24930.1 68415.m02981 Ulp1 protease family protein contains P... 28 4.7 At3g26300.1 68416.m03282 cytochrome P450 family protein contains... 27 6.2 At2g17580.1 68415.m02034 polynucleotide adenylyltransferase fami... 27 6.2 At5g50780.1 68418.m06291 ATP-binding region, ATPase-like domain-... 27 8.3 At4g28910.2 68417.m04132 expressed protein 27 8.3 At4g28910.1 68417.m04131 expressed protein 27 8.3 At2g42160.1 68415.m05218 zinc finger (ubiquitin-hydrolase) domai... 27 8.3 At2g24190.1 68415.m02890 short-chain dehydrogenase/reductase (SD... 27 8.3 >At2g02580.1 68415.m00198 cytochrome P450 family protein Length = 500 Score = 31.5 bits (68), Expect = 0.38 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +3 Query: 21 LKLIFICAIVYYADAHKNNKRQTEQKPAEPDGPV 122 L L+F+C I+ A HK + +Q P+ P P+ Sbjct: 7 LSLLFLCCILLAAFKHKKRRTNQQQPPSPPGFPI 40 >At5g15860.2 68418.m01856 expressed protein Length = 299 Score = 29.1 bits (62), Expect = 2.0 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +2 Query: 329 YVDEXCLRINVYTPSNNVSKPXPVIFYIHAGA-XYSMTGRSDLAGPYYLLDRDLVLVTIN 505 Y D+ R+++Y PSNN PV+ ++ GA L G L +RD+++ ++ Sbjct: 135 YGDQPRNRLDLYLPSNN-DGLKPVVVFVTGGAWIIGYKAWGSLLG-MQLAERDIIVACLD 192 Query: 506 Y 508 Y Sbjct: 193 Y 193 >At5g15860.1 68418.m01855 expressed protein Length = 427 Score = 29.1 bits (62), Expect = 2.0 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +2 Query: 329 YVDEXCLRINVYTPSNNVSKPXPVIFYIHAGA-XYSMTGRSDLAGPYYLLDRDLVLVTIN 505 Y D+ R+++Y PSNN PV+ ++ GA L G L +RD+++ ++ Sbjct: 135 YGDQPRNRLDLYLPSNN-DGLKPVVVFVTGGAWIIGYKAWGSLLG-MQLAERDIIVACLD 192 Query: 506 Y 508 Y Sbjct: 193 Y 193 >At1g76590.1 68414.m08912 zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI:16117799; contains Pfam profile PF04640 : Protein of unknown function, DUF597 Length = 245 Score = 28.7 bits (61), Expect = 2.7 Identities = 16/44 (36%), Positives = 21/44 (47%) Frame = -2 Query: 208 YLMPL*AWNCFPRRVSIHEPKNEPDCERVTGPSGSAGFCSVCLL 77 +L+P+ N F SIH N+ +C S FCS CLL Sbjct: 20 WLIPMLRANYFIP-CSIHAASNKSECNMFCLDCSSEAFCSYCLL 62 >At3g61220.1 68416.m06851 short-chain dehydrogenase/reductase (SDR) family protein similar to carbonyl reductase GI:1049108 from [Mus musculus] Length = 296 Score = 28.3 bits (60), Expect = 3.6 Identities = 14/44 (31%), Positives = 20/44 (45%) Frame = +2 Query: 272 RVDASKEGPACPLPVPPTYYVDEXCLRINVYTPSNNVSKPXPVI 403 R A KEG + TY + E C++IN Y P P++ Sbjct: 108 RAGAGKEGFKWDEIITETYELTEECIKINYYGPKRMCEAFIPLL 151 >At3g10630.1 68416.m01278 glycosyl transferase family 1 protein contains Pfam glycosyl transferase, group 1 family protein domain PF00534; C-terminal portion similar to mannosyltransferase GB:BAA28328 [Escherichia coli] Length = 487 Score = 28.3 bits (60), Expect = 3.6 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +3 Query: 192 YRGIRYAEPPVGKLRFQPPKLFGNTKNALTRAKRAR 299 + G ++AEP V KLR ++ N A + KR R Sbjct: 422 FEGHQWAEPSVDKLRVLMRRVMSNPDEAKVKGKRGR 457 >At1g62440.1 68414.m07044 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 826 Score = 28.3 bits (60), Expect = 3.6 Identities = 15/36 (41%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +2 Query: 305 PLPVPPTYY-VDEXCLRINVYTPSNNVSKPXPVIFY 409 P P PPTYY V VY P S P P ++Y Sbjct: 619 PPPPPPTYYAVQSPPPPPPVYYPPVTASPPPPPVYY 654 >At1g35940.1 68414.m04463 AT hook motif-containing protein-related very low similarity to SP|Q9UUA2 DNA repair and recombination protein pif1, mitochondrial precursor {Schizosaccharomyces pombe}; contains weak hit to PF02178: AT hook motif Length = 1678 Score = 28.3 bits (60), Expect = 3.6 Identities = 18/62 (29%), Positives = 35/62 (56%), Gaps = 5/62 (8%) Frame = -2 Query: 397 RXRLRDIVTRSIHVDSQAVFVDVVS-----GRDGQRAGRALFARVNAFFVLPNSFGGWNR 233 + + D++ R +VDSQ + ++ G++G+RA L+A + A+F ++ G N+ Sbjct: 1027 KSNIEDVLERVANVDSQFMAWLTLNRRNAVGKNGKRARECLYAEIPAYF----TWDGENK 1082 Query: 232 SF 227 SF Sbjct: 1083 SF 1084 >At2g24930.1 68415.m02981 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 926 Score = 27.9 bits (59), Expect = 4.7 Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 1/73 (1%) Frame = +3 Query: 75 NKRQTEQKPAEPDGPVTRSQSG-SFLGSWMETRRGKQFQAYRGIRYAEPPVGKLRFQPPK 251 N+R +KP P+ P + + + L TRRG+ + + P K+R PK Sbjct: 516 NRRLRSRKPPSPNVPKHKKEKTLNELIQKPPTRRGRGRKPLEQPKKVPPTALKIRINKPK 575 Query: 252 LFGNTKNALTRAK 290 TK+ AK Sbjct: 576 PSEETKSKAEEAK 588 >At3g26300.1 68416.m03282 cytochrome P450 family protein contains Pfam profile: PF00067 cytochrome P450 Length = 500 Score = 27.5 bits (58), Expect = 6.2 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 18 LLKLIFICAIVYYADAHKNNKRQTEQKPAEPDGPV 122 LL LIF+ I+ HKN + P+ P P+ Sbjct: 6 LLSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPI 40 >At2g17580.1 68415.m02034 polynucleotide adenylyltransferase family protein similar to SP|P13685 Poly(A) polymerase (EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam profile PF01743: polyA polymerase family protein Length = 757 Score = 27.5 bits (58), Expect = 6.2 Identities = 14/41 (34%), Positives = 24/41 (58%) Frame = +3 Query: 60 DAHKNNKRQTEQKPAEPDGPVTRSQSGSFLGSWMETRRGKQ 182 D+ +NN ++TE P P GP ++Q S + ++ RR K+ Sbjct: 593 DSEQNNDQETEVCPRTPSGP-AKNQDQSVV-QMLKRRRSKE 631 >At5g50780.1 68418.m06291 ATP-binding region, ATPase-like domain-containing protein low similarity to microrchidia [Homo sapiens] GI:5410257; contains Pfam profile PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein Length = 823 Score = 27.1 bits (57), Expect = 8.3 Identities = 18/49 (36%), Positives = 23/49 (46%) Frame = -2 Query: 532 YXNLKSXAVVDGXXHQISVQQVVRPGQVAPARHGVXRSGVNVKYHRXRL 386 Y NL + + H + VV G V A+H V G NV YH+ RL Sbjct: 457 YSNLYNLKIWLLVLHVSQLSAVVTIGFVKDAKHHVDVQGFNV-YHKNRL 504 >At4g28910.2 68417.m04132 expressed protein Length = 425 Score = 27.1 bits (57), Expect = 8.3 Identities = 13/27 (48%), Positives = 14/27 (51%) Frame = +3 Query: 60 DAHKNNKRQTEQKPAEPDGPVTRSQSG 140 D KN T Q+PAEP RS SG Sbjct: 50 DDFKNFLHPTSQRPAEPSSGSQRSDSG 76 >At4g28910.1 68417.m04131 expressed protein Length = 425 Score = 27.1 bits (57), Expect = 8.3 Identities = 13/27 (48%), Positives = 14/27 (51%) Frame = +3 Query: 60 DAHKNNKRQTEQKPAEPDGPVTRSQSG 140 D KN T Q+PAEP RS SG Sbjct: 50 DDFKNFLHPTSQRPAEPSSGSQRSDSG 76 >At2g42160.1 68415.m05218 zinc finger (ubiquitin-hydrolase) domain-containing protein similar to BRCA1-associated protein 2 [Homo sapiens] GI:3252872; contains Pfam profile PF02148: Zn-finger in ubiquitin-hydrolases and other protein Length = 488 Score = 27.1 bits (57), Expect = 8.3 Identities = 10/29 (34%), Positives = 16/29 (55%) Frame = +2 Query: 236 IPAPKTIRQYKERVDASKEGPACPLPVPP 322 I A KT+++ D +EG P+P+ P Sbjct: 444 IEAKKTLKKMSSDTDGIREGTVLPVPISP 472 >At2g24190.1 68415.m02890 short-chain dehydrogenase/reductase (SDR) family protein contains INTERPRO family IPR002198 short-chain dehydrogenase/reductase (SDR) superfamily Length = 296 Score = 27.1 bits (57), Expect = 8.3 Identities = 13/44 (29%), Positives = 19/44 (43%) Frame = +2 Query: 272 RVDASKEGPACPLPVPPTYYVDEXCLRINVYTPSNNVSKPXPVI 403 R KEG + TY + E C++IN Y P P++ Sbjct: 108 RAGTGKEGFKWEETITETYELAEECIKINYYGPKRMCEAFIPLL 151 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,617,915 Number of Sequences: 28952 Number of extensions: 248515 Number of successful extensions: 639 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 622 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 638 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1033331880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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