BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0357.Seq (348 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar... 68 5e-13 SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha... 27 0.83 SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 26 1.5 SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su... 25 3.4 SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 24 5.9 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 24 5.9 >SPAC343.14c |||translation initiation factor eIF2B|Schizosaccharomyces pombe|chr 1|||Manual Length = 393 Score = 67.7 bits (158), Expect = 5e-13 Identities = 40/111 (36%), Positives = 65/111 (58%), Gaps = 3/111 (2%) Frame = +3 Query: 9 ELETMRSSITSQAREHVRADEVLLTFGASALVERFLR-PTQNRNCKVVVAEGTDVGE--S 179 EL+ + + I Q+ +H+ ++E++LT G S VE FLR + R V+VAEG + S Sbjct: 183 ELDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAFLRFAAKKRKFSVIVAEGFPNNQKGS 242 Query: 180 HAMARRLSNSGVSVTVINSSCVFAVCPXSXRWWXRCMLRXGXCAVLGDSGL 332 HAMA+RL+ +G+ TVI+ + +FA+ + + G A+LG+ GL Sbjct: 243 HAMAKRLAQAGIDTTVISDATIFAIMSRVNK------VILGTHAILGNGGL 287 >SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccharomyces pombe|chr 3|||Manual Length = 341 Score = 27.1 bits (57), Expect = 0.83 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +1 Query: 73 CYSRSGPALWSSGS*GRPRIGTARSSSRKGLTSARVTP 186 C+ ++WS GS + T +SSS KG+T V P Sbjct: 95 CHDNGQISMWSKGS--WLLVHTLKSSSHKGITGIAVHP 130 >SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 629 Score = 26.2 bits (55), Expect = 1.5 Identities = 7/23 (30%), Positives = 11/23 (47%) Frame = +3 Query: 246 FAVCPXSXRWWXRCMLRXGXCAV 314 F +C + WW R + G C + Sbjct: 545 FQLCSENYNWWWRSFITPGFCGI 567 >SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Sum3|Schizosaccharomyces pombe|chr 3|||Manual Length = 636 Score = 25.0 bits (52), Expect = 3.4 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -2 Query: 149 DDLAVPILGRPQEPLDQSAGPERE*HLVRPHVLPGLTR 36 DD+ V + G EP+++ P HL++ L G T+ Sbjct: 154 DDIPVEVSGGDIEPVNEFTSPPLNSHLLQNIKLSGYTQ 191 >SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 2073 Score = 24.2 bits (50), Expect = 5.9 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = -3 Query: 175 SPTSVPSATTTLQFLFWVGLRNRST 101 S + + +A L LFW+G+R++ T Sbjct: 285 SASFMENAKVALTTLFWIGVRSQQT 309 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 24.2 bits (50), Expect = 5.9 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = -3 Query: 265 DXGHTANTHELLMTVTETPELLSRRAMA*LSPTSVPSAT 149 D T NTHE + +PE A A P+S+PS + Sbjct: 214 DRSQTKNTHEETSPIVSSPE---DDADAAFKPSSLPSTS 249 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,134,755 Number of Sequences: 5004 Number of extensions: 16752 Number of successful extensions: 41 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 41 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 104153322 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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