Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0357.Seq
         (348 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g07300.1 68416.m00869 eukaryotic translation initiation facto...    56   5e-09
At1g48970.1 68414.m05489 eukaryotic translation initiation facto...    33   0.069
At2g44070.1 68415.m05481 eukaryotic translation initiation facto...    30   0.37 
At5g38640.1 68418.m04673 eukaryotic translation initiation facto...    29   0.64 
At2g24170.1 68415.m02888 endomembrane protein 70, putative simil...    28   2.0  
At5g48670.1 68418.m06023 MADS-box family protein contains simila...    27   4.5  
At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f...    27   4.5  
At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4 f...    26   6.0  
At4g34980.1 68417.m04959 subtilase family protein similar to SBT...    26   7.9  

>At3g07300.1 68416.m00869 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|P49770 Translation initiation factor eIF-2B beta
           subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF01008: Initiation factor 2
           subunit family
          Length = 407

 Score = 56.4 bits (130), Expect = 5e-09
 Identities = 32/84 (38%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
 Frame = +3

Query: 9   ELETMRSSITSQAREHVRADEVLLTFGASALVERFL--RPTQNRNCKVVVAEGTDVGESH 182
           E+      I  QA EH+  +EV+LT G+S  V  FL     + R+ +V VAEG    + H
Sbjct: 190 EIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQGH 249

Query: 183 AMARRLSNSGVSVTVINSSCVFAV 254
            +A+ L   G+  TVI  S VFA+
Sbjct: 250 LLAKELVARGLQTTVITDSAVFAM 273


>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           guanine nucleotide exchange factor, eIF-2B, delta
           subunit [Mus musculus] GI:529428; contains Pfam profile
           PF01008: Initiation factor 2 subunit family
          Length = 756

 Score = 32.7 bits (71), Expect = 0.069
 Identities = 21/74 (28%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
 Frame = +3

Query: 30  SITSQAREHVRADEVLLTFGASALVERFLRPTQ--NRNCKVVVAEGTDVGESHAMARRLS 203
           +I   A   +R  EVLLT+G S +VE  L       +  +VV+ +     E   + RRL 
Sbjct: 540 AIVQHAVTKIRDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLV 599

Query: 204 NSGVSVTVINSSCV 245
             G+  T  + + +
Sbjct: 600 TRGLDCTYTHINAI 613


>At2g44070.1 68415.m05481 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|Q63186 Translation initiation factor eIF-2B delta
           subunit (eIF-2B GDP-GTP exchange factor) {Rattus
           norvegicus}; contains Pfam profile PF01008: Initiation
           factor 2 subunit family
          Length = 307

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 18/73 (24%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
 Frame = +3

Query: 33  ITSQAREHVRADEVLLTFGASALVERFLRPTQ--NRNCKVVVAEGTDVGESHAMARRLSN 206
           I   A   +R  +VLLT+G+S ++E  L       +  +V V +     +   + RRL  
Sbjct: 91  IVEHAVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIK 150

Query: 207 SGVSVTVINSSCV 245
            G++ T  + + +
Sbjct: 151 RGINCTYTHITAI 163


>At5g38640.1 68418.m04673 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           SP|P41111 Translation initiation factor eIF-2B delta
           subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF01008: Initiation
           factor 2 subunit family
          Length = 642

 Score = 29.5 bits (63), Expect = 0.64
 Identities = 18/73 (24%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
 Frame = +3

Query: 33  ITSQAREHVRADEVLLTFGASALVERFLRPTQ--NRNCKVVVAEGTDVGESHAMARRLSN 206
           I   A   +R  +VLLT+G+   VE  +       +  +V+V +     E   + RRL  
Sbjct: 433 IVKHAVTKIRDGDVLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIK 492

Query: 207 SGVSVTVINSSCV 245
            G++ T  + + +
Sbjct: 493 RGINCTYTHINAI 505


>At2g24170.1 68415.m02888 endomembrane protein 70, putative similar
           to  MURA transposase of maize Mutator transposon
          Length = 637

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 10/25 (40%), Positives = 12/25 (48%)
 Frame = +3

Query: 240 CVFAVCPXSXRWWXRCMLRXGXCAV 314
           C F +C    +WW R  L  G  AV
Sbjct: 551 CYFQLCSEDYQWWWRSYLTSGSSAV 575


>At5g48670.1 68418.m06023 MADS-box family protein contains
           similarity to MADS-box DNA-binding protein
          Length = 321

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 11/39 (28%), Positives = 24/39 (61%)
 Frame = +3

Query: 99  LVERFLRPTQNRNCKVVVAEGTDVGESHAMARRLSNSGV 215
           ++E++L+   NR  +++   GT++GES ++A   S   +
Sbjct: 141 MIEQYLKDV-NRRIEILRNSGTEIGESSSVAVAASEGNI 178


>At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4
           family;
          Length = 644

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 10/25 (40%), Positives = 11/25 (44%)
 Frame = +3

Query: 240 CVFAVCPXSXRWWXRCMLRXGXCAV 314
           C F +C     WW R  L  G  AV
Sbjct: 558 CYFQLCSEDYLWWWRSYLTSGSSAV 582


>At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4
           family;
          Length = 641

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 9/24 (37%), Positives = 10/24 (41%)
 Frame = +3

Query: 240 CVFAVCPXSXRWWXRCMLRXGXCA 311
           C F +C     WW R  L  G  A
Sbjct: 555 CYFQLCSEDYNWWWRAYLTAGSSA 578


>At4g34980.1 68417.m04959 subtilase family protein similar to SBT1,
           a subtilase from tomato plants GI:1771160 from
           [Lycopersicon esculentum]
          Length = 764

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 19/47 (40%), Positives = 25/47 (53%), Gaps = 4/47 (8%)
 Frame = +3

Query: 120 PTQNRNC--KVVVAEGTDVGESHAM--ARRLSNSGVSVTVINSSCVF 248
           PT  R    K V+   T+VG++ A+  AR  S  GV+VTV     VF
Sbjct: 664 PTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVF 710


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,323,972
Number of Sequences: 28952
Number of extensions: 101264
Number of successful extensions: 290
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 275
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 290
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 429398688
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)