Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0355.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 175 4e-45
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 175 4e-45
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 175 4e-45
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 98 9e-22
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 88 9e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 56 3e-09
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 2e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 7e-04
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.016
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 29 0.34
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 29 0.34
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 29 0.34
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 1.4
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 27 1.4
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 2.4
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 4.2
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 5.6
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 7.3
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 25 7.3
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 7.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 175 bits (426), Expect = 4e-45
Identities = 80/85 (94%), Positives = 82/85 (96%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDI
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDI 75
Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255
ALWKFET KY VT+IDAPGHRDFIK
Sbjct: 76 ALWKFETPKYNVTVIDAPGHRDFIK 100
Score = 122 bits (293), Expect = 5e-29
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +3
Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425
F +NMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQ IV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494
T +S+ RFEE KE S++I++
Sbjct: 158 T--GWSQARFEEIVKETSNFIKK 178
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 490 KKIGLQPTDDXFVPISGW 543
KK+G P FVP+SG+
Sbjct: 177 KKVGFNPKTVPFVPVSGF 194
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 175 bits (426), Expect = 4e-45
Identities = 80/85 (94%), Positives = 82/85 (96%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDI
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDI 75
Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255
ALWKFET KY VT+IDAPGHRDFIK
Sbjct: 76 ALWKFETPKYNVTVIDAPGHRDFIK 100
Score = 122 bits (294), Expect = 3e-29
Identities = 60/83 (72%), Positives = 71/83 (85%)
Frame = +3
Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425
F +NMITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQ IV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494
T +S+ RFEE KE S++I++
Sbjct: 158 T--GWSQARFEEIVKETSNFIKK 178
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 490 KKIGLQPTDDXFVPISGW 543
KK+G P FVP+SG+
Sbjct: 177 KKVGFNPKTVPFVPVSGF 194
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 175 bits (426), Expect = 4e-45
Identities = 80/85 (94%), Positives = 82/85 (96%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDI
Sbjct: 16 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDI 75
Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255
ALWKFET KY VT+IDAPGHRDFIK
Sbjct: 76 ALWKFETPKYNVTVIDAPGHRDFIK 100
Score = 122 bits (293), Expect = 5e-29
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +3
Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425
F +NMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQ IV VNKMD+
Sbjct: 98 FIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDT 157
Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494
T +S+ RFEE KE S++I++
Sbjct: 158 T--GWSQARFEEIVKETSNFIKK 178
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 490 KKIGLQPTDDXFVPISGW 543
KK+G P FVP+SG+
Sbjct: 177 KKVGFNPKTVPFVPVSGF 194
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 97.9 bits (233), Expect = 9e-22
Identities = 44/84 (52%), Positives = 58/84 (69%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
VDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD + ER RG+T+D+
Sbjct: 186 VDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDV 245
Query: 181 ALWKFETSKYYVTIIDAPGHRDFI 252
A FE+ K I DAPGHRDFI
Sbjct: 246 ASTTFESDKKIYEIGDAPGHRDFI 269
Score = 74.5 bits (175), Expect = 9e-15
Identities = 38/81 (46%), Positives = 49/81 (60%)
Frame = +3
Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425
F MI G S AD AVL+V + FE G +NGQTREHA L LG+ + +V VNK+D
Sbjct: 268 FISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDL 327
Query: 426 TEPPYSEPRFEEXKKEVSSYI 488
+SE RF+E K VS ++
Sbjct: 328 MS--WSEDRFQEIKNIVSDFL 346
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 87.8 bits (208), Expect = 9e-19
Identities = 37/84 (44%), Positives = 58/84 (69%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
VD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD ERE+G T+++
Sbjct: 247 VDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEV 306
Query: 181 ALWKFETSKYYVTIIDAPGHRDFI 252
FET +++DAPGH+ ++
Sbjct: 307 GRAYFETEHRRFSLLDAPGHKGYV 330
Score = 84.6 bits (200), Expect = 9e-18
Identities = 38/79 (48%), Positives = 54/79 (68%)
Frame = +3
Query: 255 NMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDSTEP 434
NMI G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ +V +NKMD
Sbjct: 332 NMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSV 391
Query: 435 PYSEPRFEEXKKEVSSYIQ 491
+SE R++E ++S +++
Sbjct: 392 QWSEERYKECVDKLSMFLR 410
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 56.4 bits (130), Expect = 3e-09
Identities = 34/89 (38%), Positives = 49/89 (55%)
Frame = +3
Query: 219 HH*CSWTQRFHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQX 398
H C + +NMITG + D A+++V+A G+ QTREH LLA +GVKQ
Sbjct: 120 HVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQI 172
Query: 399 IVGVNKMDSTEPPYSEPRFEEXKKEVSSY 485
+V +NK+D EP E E ++ +S Y
Sbjct: 173 VVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 46.0 bits (104), Expect = 4e-06
Identities = 21/53 (39%), Positives = 32/53 (60%)
Frame = +1
Query: 97 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIK 255
++G+ SF +DK E+ RGITI A ++ET+ + +D PGH D+IK
Sbjct: 79 DLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIK 131
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 2e-05
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1
Query: 130 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 249
V+D L AER+RGITI+ A F + +ID PGH DF
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADF 106
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 7e-04
Identities = 18/43 (41%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +1
Query: 133 LDKLKAERERGITIDI----ALWKFETSKYYVTIIDAPGHRDF 249
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDF 137
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.9 bits (74), Expect = 0.016
Identities = 34/102 (33%), Positives = 47/102 (46%), Gaps = 19/102 (18%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180
+DSGK+T T ++Y G I K+ E+ +G +D ++ ERE+GITI
Sbjct: 68 IDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELEREKGITIQS 117
Query: 181 A----LWK---------------FETSKYYVTIIDAPGHRDF 249
A W+ FE S Y + IID PGH DF
Sbjct: 118 AATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDF 158
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 29.5 bits (63), Expect = 0.34
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -1
Query: 173 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIPPH 45
I IP+ A S+ N++ + +D LP + S V+L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 29.5 bits (63), Expect = 0.34
Identities = 28/86 (32%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGID--KRTIEKF-EKEAQEMGKGSFKYAWVLDKLKAERERGIT 171
VD GKST T L+ K G I K +F + A E +G + + L AE
Sbjct: 28 VDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAIS-LFAEMTDDDM 86
Query: 172 IDIALWKFETSKYYVTIIDAPGHRDF 249
D+ + + + V +ID+PGH DF
Sbjct: 87 KDMKE-PADGTDFLVNLIDSPGHVDF 111
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 29.5 bits (63), Expect = 0.34
Identities = 28/86 (32%), Positives = 40/86 (46%), Gaps = 3/86 (3%)
Frame = +1
Query: 1 VDSGKSTTTGHLIYKCGGID--KRTIEKF-EKEAQEMGKGSFKYAWVLDKLKAERERGIT 171
VD GKST T L+ K G I K +F + A E +G + + L AE
Sbjct: 28 VDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAIS-LFAEMTDDDM 86
Query: 172 IDIALWKFETSKYYVTIIDAPGHRDF 249
D+ + + + V +ID+PGH DF
Sbjct: 87 KDMKE-PADGTDFLVNLIDSPGHVDF 111
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 27.5 bits (58), Expect = 1.4
Identities = 18/80 (22%), Positives = 33/80 (41%)
Frame = +3
Query: 258 MITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDSTEPP 437
M+ G + D A+L++A QT EH + +K I+ NK+D
Sbjct: 125 MLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQNKVDLIRES 178
Query: 438 YSEPRFEEXKKEVSSYIQED 497
+E ++ K + + E+
Sbjct: 179 AAEEHYQSILKFIKGTVAEN 198
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 27.5 bits (58), Expect = 1.4
Identities = 14/42 (33%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = +1
Query: 136 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFI 252
+K++A+ E+ + I+I + E +++ T+ID PG DFI
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFI 101
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 2.4
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -1
Query: 197 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMV 66
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 4.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -2
Query: 163 HAHAQPLVCPIPKHI*RILYPFPGPPSRTSRWY 65
H+H PL P+P +L P P P S WY
Sbjct: 135 HSHHPPLHNPLPVSCQPVLRP-PPVPQVPSHWY 166
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 5.6
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -2
Query: 142 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 14
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 7.3
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 97 PGPPSRTSRWYVCQYHHIC 41
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 25.0 bits (52), Expect = 7.3
Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +1
Query: 103 GKGSFKYAWVLDKLKAERERGITIDIALWK--FETSKYYVTIIDAPGHRDF 249
GK + A+ + + GIT I + F+ ++T +D PGH F
Sbjct: 183 GKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAF 233
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 7.3
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 208 YYVTIIDAPGHRDF 249
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,313,308
Number of Sequences: 5004
Number of extensions: 45766
Number of successful extensions: 132
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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