BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0355.Seq (548 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 166 1e-41 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 166 1e-41 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 166 1e-41 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 166 1e-41 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 100 6e-22 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 82 3e-16 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 44 5e-05 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 44 7e-05 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 44 7e-05 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 44 7e-05 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 42 2e-04 At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 41 5e-04 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 35 0.031 At5g13650.2 68418.m01585 elongation factor family protein contai... 35 0.041 At5g13650.1 68418.m01584 elongation factor family protein contai... 35 0.041 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.17 At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 27 6.2 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 166 bits (403), Expect = 1e-41 Identities = 76/85 (89%), Positives = 79/85 (92%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDI Sbjct: 16 VDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDI 75 Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255 ALWKFET+KYY T+IDAPGHRDFIK Sbjct: 76 ALWKFETTKYYCTVIDAPGHRDFIK 100 Score = 122 bits (294), Expect = 2e-28 Identities = 59/83 (71%), Positives = 70/83 (84%) Frame = +3 Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425 F +NMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ I NKMD+ Sbjct: 98 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDA 157 Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494 T P YS+ R++E KEVSSY+++ Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKK 180 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 166 bits (403), Expect = 1e-41 Identities = 76/85 (89%), Positives = 79/85 (92%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDI Sbjct: 16 VDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDI 75 Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255 ALWKFET+KYY T+IDAPGHRDFIK Sbjct: 76 ALWKFETTKYYCTVIDAPGHRDFIK 100 Score = 122 bits (294), Expect = 2e-28 Identities = 59/83 (71%), Positives = 70/83 (84%) Frame = +3 Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425 F +NMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ I NKMD+ Sbjct: 98 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDA 157 Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494 T P YS+ R++E KEVSSY+++ Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKK 180 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 166 bits (403), Expect = 1e-41 Identities = 76/85 (89%), Positives = 79/85 (92%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDI Sbjct: 16 VDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDI 75 Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255 ALWKFET+KYY T+IDAPGHRDFIK Sbjct: 76 ALWKFETTKYYCTVIDAPGHRDFIK 100 Score = 122 bits (294), Expect = 2e-28 Identities = 59/83 (71%), Positives = 70/83 (84%) Frame = +3 Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425 F +NMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ I NKMD+ Sbjct: 98 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDA 157 Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494 T P YS+ R++E KEVSSY+++ Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKK 180 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 166 bits (403), Expect = 1e-41 Identities = 76/85 (89%), Positives = 79/85 (92%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDI Sbjct: 16 VDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDI 75 Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255 ALWKFET+KYY T+IDAPGHRDFIK Sbjct: 76 ALWKFETTKYYCTVIDAPGHRDFIK 100 Score = 122 bits (294), Expect = 2e-28 Identities = 59/83 (71%), Positives = 70/83 (84%) Frame = +3 Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDS 425 F +NMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ I NKMD+ Sbjct: 98 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDA 157 Query: 426 TEPPYSEPRFEEXKKEVSSYIQE 494 T P YS+ R++E KEVSSY+++ Sbjct: 158 TTPKYSKARYDEIIKEVSSYLKK 180 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 100 bits (240), Expect = 6e-22 Identities = 42/84 (50%), Positives = 63/84 (75%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+ ERERGIT+ + Sbjct: 248 VDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAEERERGITMTV 307 Query: 181 ALWKFETSKYYVTIIDAPGHRDFI 252 A+ F + +++V ++D+PGH+DF+ Sbjct: 308 AVAYFNSKRHHVVLLDSPGHKDFV 331 Score = 76.2 bits (179), Expect = 1e-14 Identities = 40/83 (48%), Positives = 52/83 (62%), Gaps = 1/83 (1%) Frame = +3 Query: 246 FHQNMITGTSQADCAVLIVAAGTGEFEAGISK-NGQTREHALLAFTLGVKQXIVGVNKMD 422 F NMI G +QAD A+L++ A G FEAG GQTREHA + GV+Q IV +NKMD Sbjct: 330 FVPNMIAGATQADAAILVIDASVGAFEAGFDNLKGQTREHARVLRGFGVEQVIVAINKMD 389 Query: 423 STEPPYSEPRFEEXKKEVSSYIQ 491 YS+ RF+ K+ V S++Q Sbjct: 390 IV--GYSKERFDLIKQHVGSFLQ 410 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 81.8 bits (193), Expect = 3e-16 Identities = 38/79 (48%), Positives = 55/79 (69%) Frame = +3 Query: 255 NMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIVGVNKMDSTEP 434 NMI+G SQAD VL+++A GEFE G + GQTREH LA TLGV + IV VNKMD Sbjct: 195 NMISGASQADIGVLVISARKGEFETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTV 254 Query: 435 PYSEPRFEEXKKEVSSYIQ 491 +S+ R++E ++++ +++ Sbjct: 255 NWSKERYDEIEQKMVPFLK 273 Score = 78.2 bits (184), Expect = 3e-15 Identities = 33/84 (39%), Positives = 56/84 (66%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D + ER +G T+++ Sbjct: 110 VDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTNEEERLKGKTVEV 169 Query: 181 ALWKFETSKYYVTIIDAPGHRDFI 252 FET TI+DAPGH+ ++ Sbjct: 170 GRAHFETESTRFTILDAPGHKSYV 193 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 44.4 bits (100), Expect = 5e-05 Identities = 31/90 (34%), Positives = 45/90 (50%), Gaps = 1/90 (1%) Frame = +3 Query: 219 HH*CSWTQRFHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQX 398 H C + +NMITG +Q D A+L+V+ G QT+EH LLA +GV Sbjct: 146 HVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP-------QTKEHILLAKQVGVPDM 198 Query: 399 IVGVNKMDSTEPPYSEPRFE-EXKKEVSSY 485 +V +NK D + E E ++ +SSY Sbjct: 199 VVFLNKEDQVDDAELLELVELEVRELLSSY 228 Score = 42.3 bits (95), Expect = 2e-04 Identities = 26/85 (30%), Positives = 39/85 (45%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VD GK+T T L I +K+++ +D ER RGITI+ Sbjct: 88 VDHGKTTLTAALTMALASIGSSVAKKYDE---------------IDAAPEERARGITINT 132 Query: 181 ALWKFETSKYYVTIIDAPGHRDFIK 255 A ++ET + +D PGH D++K Sbjct: 133 ATVEYETENRHYAHVDCPGHADYVK 157 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 44.0 bits (99), Expect = 7e-05 Identities = 18/41 (43%), Positives = 28/41 (68%) Frame = +1 Query: 133 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIK 255 +DK E++RGITI A ++ET+K + +D PGH D++K Sbjct: 105 IDKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVK 145 Score = 43.2 bits (97), Expect = 1e-04 Identities = 28/89 (31%), Positives = 43/89 (48%) Frame = +3 Query: 219 HH*CSWTQRFHQNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQX 398 H C + +NMITG +Q D +L+V+ G QT+EH LLA +GV Sbjct: 134 HVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP-------QTKEHILLARQVGVPSL 186 Query: 399 IVGVNKMDSTEPPYSEPRFEEXKKEVSSY 485 + +NK+D + P E +E+ S+ Sbjct: 187 VCFLNKVDVVDDPELLELVEMELRELLSF 215 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 44.0 bits (99), Expect = 7e-05 Identities = 31/83 (37%), Positives = 42/83 (50%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 +DSGK+T T +++ G I E E ++ G G+ +D + ERE+GITI Sbjct: 74 IDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLEREKGITIQS 123 Query: 181 ALWKFETSKYYVTIIDAPGHRDF 249 A Y V IID PGH DF Sbjct: 124 AATYCTWKDYKVNIIDTPGHVDF 146 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 44.0 bits (99), Expect = 7e-05 Identities = 31/83 (37%), Positives = 42/83 (50%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 +DSGK+T T +++ G I E E ++ G G+ +D + ERE+GITI Sbjct: 74 IDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLEREKGITIQS 123 Query: 181 ALWKFETSKYYVTIIDAPGHRDF 249 A Y V IID PGH DF Sbjct: 124 AATYCTWKDYKVNIIDTPGHVDF 146 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 42.3 bits (95), Expect = 2e-04 Identities = 28/83 (33%), Positives = 42/83 (50%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 +D+GK+TTT ++Y G + E+ +G+ W ++ E+ERGITI Sbjct: 105 IDAGKTTTTERILYYTGR---------NYKIGEVHEGTATMDW----MEQEQERGITITS 151 Query: 181 ALWKFETSKYYVTIIDAPGHRDF 249 A K+ + IID PGH DF Sbjct: 152 AATTTFWDKHRINIIDTPGHVDF 174 >At5g08650.1 68418.m01029 GTP-binding protein LepA, putative Length = 681 Score = 41.1 bits (92), Expect = 5e-04 Identities = 29/79 (36%), Positives = 41/79 (51%), Gaps = 4/79 (5%) Frame = +1 Query: 133 LDKLKAERERGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKT*SQEPLRLIALCSS 300 LD + ERERGITI + + +E + + + +ID PGH DF S E R +A C Sbjct: 123 LDNMDLERERGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDF----SYEVSRSLAACEG 178 Query: 301 *LPVPVNSKLVSLRTVKPV 357 L V S+ V +T+ V Sbjct: 179 ALLVVDASQGVEAQTLANV 197 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 35.1 bits (77), Expect = 0.031 Identities = 27/83 (32%), Positives = 37/83 (44%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 VD GK+T HLI GG + GK F +D L E+ R IT+ Sbjct: 18 VDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQRRAITMKS 64 Query: 181 ALWKFETSKYYVTIIDAPGHRDF 249 + + Y + +ID+PGH DF Sbjct: 65 SSISLKYKDYSLNLIDSPGHMDF 87 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 34.7 bits (76), Expect = 0.041 Identities = 18/40 (45%), Positives = 20/40 (50%) Frame = +1 Query: 130 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 249 ++D ERERGITI V IID PGH DF Sbjct: 120 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDF 159 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 34.7 bits (76), Expect = 0.041 Identities = 18/40 (45%), Positives = 20/40 (50%) Frame = +1 Query: 130 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 249 ++D ERERGITI V IID PGH DF Sbjct: 119 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDF 158 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 32.7 bits (71), Expect = 0.17 Identities = 41/125 (32%), Positives = 53/125 (42%), Gaps = 9/125 (7%) Frame = +1 Query: 1 VDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDI 180 +D GKST L+ G I K G G +Y LDKL +RERGIT+ Sbjct: 75 IDHGKSTLADRLMELTGTIKK-------------GHGQPQY---LDKL--QRERGITVKA 116 Query: 181 ALWKF---------ETSKYYVTIIDAPGHRDFIKT*SQEPLRLIALCSS*LPVPVNSKLV 333 E S Y + +ID PGH DF S E R ++ C L V ++ V Sbjct: 117 QTATMFYENKVEDQEASGYLLNLIDTPGHVDF----SYEVSRSLSACQGALLVVDAAQGV 172 Query: 334 SLRTV 348 +TV Sbjct: 173 QAQTV 177 >At3g01360.1 68416.m00057 expressed protein contains Pfam profile PF04819: Family of unknown function (DUF716) (Plant viral-response family) Length = 319 Score = 27.5 bits (58), Expect = 6.2 Identities = 19/69 (27%), Positives = 29/69 (42%), Gaps = 1/69 (1%) Frame = +3 Query: 228 CSWTQRFHQNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQXIV 404 C +H+ T Q +C + L+V TG F +KNG R+ LG + + Sbjct: 228 CKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELENL 287 Query: 405 GVNKMDSTE 431 +DS E Sbjct: 288 STFTLDSDE 296 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,353,891 Number of Sequences: 28952 Number of extensions: 247390 Number of successful extensions: 633 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 607 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 624 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1033331880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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