BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0353.Seq (508 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 comple... 30 0.23 SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizo... 29 0.40 SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk... 27 2.1 SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb... 26 3.7 SPAC1071.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 6.5 >SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 442 Score = 29.9 bits (64), Expect = 0.23 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 286 VLDHAICKSYPIHQN*RLRTRGPPSIGFDLIKALIP 179 ++D K Y IH + L + PS GF +I++L+P Sbjct: 391 LIDQGYIKGYIIHASSTLVLKKDPSFGFSVIESLMP 426 >SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizosaccharomyces pombe|chr 3|||Manual Length = 979 Score = 29.1 bits (62), Expect = 0.40 Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 3/66 (4%) Frame = +1 Query: 226 ASEVVNFDESDNFCRSHGQVPATHLSNVCLINFRW*F---LRLPWLPRVTGNQGSIPXRX 396 + E+++F E G + L C+IN W LRL +L + NQ S + Sbjct: 243 SKEIIDFLEKSKTLVELGMDSSCSLVAECMINETWPVDRALRLQFLIQQRNNQSSNEEQK 302 Query: 397 PEKRLP 414 EKR+P Sbjct: 303 QEKRVP 308 >SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 306 Score = 26.6 bits (56), Expect = 2.1 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = -2 Query: 423 PWMW*PFLRXPXRNRTLIPRYPWQPW*SQKLPS 325 P MW P N+ + YP +PW S+ LPS Sbjct: 236 PSMWPELSTFPDWNKFIFHEYPPKPW-SEILPS 267 >SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pombe|chr 1|||Manual Length = 649 Score = 25.8 bits (54), Expect = 3.7 Identities = 18/42 (42%), Positives = 25/42 (59%) Frame = -3 Query: 353 NHGSRRNYHRKLIRQTFERCVAGT*PCDLQKLSDSSKLTTSD 228 N SR+ ++ L R C+ G PC+L+ LSD+S LTT D Sbjct: 70 NKPSRKALYQAL-RSLRLNCL-GDKPCELELLSDTS-LTTGD 108 >SPAC1071.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 25.0 bits (52), Expect = 6.5 Identities = 11/35 (31%), Positives = 22/35 (62%) Frame = +3 Query: 75 NGSIYQFWFLRSYSVTWITVVILELIHAIRTLTSD 179 N IY+ +F ++S++ + + I L +RT++SD Sbjct: 14 NTQIYRIFFTLTFSLSNLFLAICYLFLNVRTVSSD 48 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,116,399 Number of Sequences: 5004 Number of extensions: 40893 Number of successful extensions: 86 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 84 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 86 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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