Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0345.Seq
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 136 3e-33
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 134 9e-33
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 107 1e-24
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 79 4e-16
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 77 1e-15
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 33 0.032
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 31 0.17
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 30 0.29
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 29 0.52
SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce... 29 0.68
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 2.1
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 27 2.1
SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc... 27 2.7
SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pomb... 27 2.7
SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ... 26 3.6
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 26 3.6
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 26 3.6
SPBC15D4.01c ||SPBC2D10.21c|kinesin-like protein|Schizosaccharom... 26 4.8
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 26 4.8
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 26 4.8
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 26 4.8
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 4.8
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.4
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 25 8.4
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 136 bits (328), Expect = 3e-33
Identities = 68/112 (60%), Positives = 83/112 (74%)
Frame = -3
Query: 590 GKFXX*PGIPPAPRGVPQI*GHLXHRCXGILNVSAIXKSTNKEXKITITNDKGRLSKEEI 411
GKF GIPPAPRGVPQI GILNVSA+ K T K KITITNDKGRLSKEEI
Sbjct: 455 GKFEL-SGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEI 513
Query: 410 ERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEKISDSDK 255
+RMV+EAEKY+ ED+ + IQAKN LESY +S++++++D LK+K+ SDK
Sbjct: 514 DRMVSEAEKYKAEDEAETSRIQAKNHLESYAYSLRNSLDDPNLKDKVDASDK 565
Score = 58.8 bits (136), Expect = 6e-10
Identities = 26/46 (56%), Positives = 33/46 (71%)
Frame = -2
Query: 258 QETILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 121
+E I +TI+WLD N A K+EYE KQKELEG+ NPI+ K+YQ
Sbjct: 565 KEAIDKAVKETIEWLDHNTTAAKDEYEDKQKELEGVANPIMAKIYQ 610
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 134 bits (324), Expect = 9e-33
Identities = 68/112 (60%), Positives = 82/112 (73%)
Frame = -3
Query: 590 GKFXX*PGIPPAPRGVPQI*GHLXHRCXGILNVSAIXKSTNKEXKITITNDKGRLSKEEI 411
GKF GIPPAPRGVPQI GILNVSA+ K T K KITITNDKGRLSKEEI
Sbjct: 455 GKFEL-SGIPPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEI 513
Query: 410 ERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEKISDSDK 255
+RMV EAEKY+ ED+ + IQAKN LESY +S++++++D LK+K+ SDK
Sbjct: 514 DRMVAEAEKYKAEDEAESGRIQAKNHLESYAYSLRNSLDDPNLKDKVDASDK 565
Score = 58.0 bits (134), Expect = 1e-09
Identities = 25/46 (54%), Positives = 34/46 (73%)
Frame = -2
Query: 258 QETILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 121
+ET+ +TI+WLDSN A K+E+E KQKELE + NPI+ K+YQ
Sbjct: 565 KETVDKAVKETIEWLDSNTTAAKDEFEAKQKELESVANPIMAKIYQ 610
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 107 bits (257), Expect = 1e-24
Identities = 59/115 (51%), Positives = 75/115 (65%), Gaps = 2/115 (1%)
Frame = -3
Query: 590 GKFXX*PGIPPAPRGVPQI*GHLXHRCXGILNVSAIXKS-TNKEXKITITNDKGRLSKEE 414
GKF GIPPAPRGVPQI G+L VSA+ KS K K+ I NDKGRLS+E+
Sbjct: 484 GKFDL-RGIPPAPRGVPQIEVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEED 542
Query: 413 IERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTM-EDEKLKEKISDSDKR 252
IERMV EAE++ ED KE I+A+N LE+Y +S+K +DE+L K+ DK+
Sbjct: 543 IERMVKEAEEFAEEDKILKERIEARNTLENYAYSLKGQFDDDEQLGGKVDPEDKQ 597
Score = 38.3 bits (85), Expect = 8e-04
Identities = 15/46 (32%), Positives = 31/46 (67%), Gaps = 1/46 (2%)
Frame = -2
Query: 258 QETILDKCNDTIKWLDSN-QLADKEEYEHKQKELEGIYNPIITKMY 124
++ +LD D +WL+ + + A KEE+E ++++L+ + +PI K+Y
Sbjct: 596 KQAVLDAVEDVAEWLEIHGEDASKEEFEDQRQKLDAVVHPITQKLY 641
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 79.4 bits (187), Expect = 4e-16
Identities = 44/97 (45%), Positives = 57/97 (58%)
Frame = -3
Query: 569 GIPPAPRGVPQI*GHLXHRCXGILNVSAIXKSTNKEXKITITNDKGRLSKEEIERMVNEA 390
GI PAP+G PQI GI+NVSA K+TNK+ IT+ G L+ EIE MV +A
Sbjct: 504 GIAPAPKGQPQIEVSFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDSEIEAMVADA 562
Query: 389 EKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLK 279
EKYR D +KE I+ N ES C ++S ++ K K
Sbjct: 563 EKYRASDMARKEAIENGNRAESVCTDIESNLDIHKDK 599
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 77.4 bits (182), Expect = 1e-15
Identities = 36/105 (34%), Positives = 61/105 (58%)
Frame = -3
Query: 569 GIPPAPRGVPQI*GHLXHRCXGILNVSAIXKSTNKEXKITITNDKGRLSKEEIERMVNEA 390
GIPP PRG ++ GIL V+A+ K+T + I ITN G LS +I+ M+ A
Sbjct: 466 GIPPMPRGQAELEATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQEMIENA 525
Query: 389 EKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEKISDSDK 255
+K++ +D + ++AK+ LESY ++++T+ + + K+ DK
Sbjct: 526 DKFKQQDKDFAKKLEAKSQLESYISNIETTISEPNVMMKLKRGDK 570
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 33.1 bits (72), Expect = 0.032
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = -3
Query: 479 KSTNKEXKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQ-KETIQAKNALESYCFSMKS 303
K K ++++ + RL E +E+ EAE DK ET+ KNALE Y + ++
Sbjct: 534 KKLVKVADLSVSVQEDRLPTEVLEKY-REAEHQMIATDKLVAETVDRKNALEEYIYDTRA 592
Query: 302 TMED 291
++D
Sbjct: 593 KLDD 596
>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 682
Score = 30.7 bits (66), Expect = 0.17
Identities = 23/69 (33%), Positives = 35/69 (50%)
Frame = -3
Query: 470 NKEXKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMED 291
NK+ K T D SKEE+E +V E E D +Q + K+ L++ F ++
Sbjct: 529 NKKGKHTQIEDPTAASKEELENLVREDE----NDSEQLDHFDMKSILKAEKFK-----KN 579
Query: 290 EKLKEKISD 264
KLK+K S+
Sbjct: 580 RKLKKKASN 588
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 29.9 bits (64), Expect = 0.29
Identities = 17/56 (30%), Positives = 32/56 (57%)
Frame = -3
Query: 425 SKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEKISDSD 258
+KEE+E +NE + E ++Q + ++N ++ +K +E EKLKE ++ D
Sbjct: 1606 TKEELENQLNEKSQRIKELEEQAQKNSSENTHDNIDDMIKQQVE-EKLKENSANFD 1660
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 29.1 bits (62), Expect = 0.52
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = -3
Query: 437 KGRLSKEEIERMVNEAEKYRNEDD 366
KG LSKEE+ER V +A K N D
Sbjct: 525 KGTLSKEELERRVYDAAKLANAYD 548
>SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 190
Score = 28.7 bits (61), Expect = 0.68
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = -3
Query: 500 LNVSAIXKSTNKEXKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE--TIQAKNA 333
LNVS S +E +T ++ R+ +EE +RM ++ E K+K T+Q +A
Sbjct: 63 LNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRMASKVLIKTIERTKRKRVTTVQGLDA 120
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 27.1 bits (57), Expect = 2.1
Identities = 13/54 (24%), Positives = 28/54 (51%)
Frame = -3
Query: 494 VSAIXKSTNKEXKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNA 333
V A+ KS +++ ++ G + EEI ++++ E E+D++ E N+
Sbjct: 597 VPALFKSIDQDESDILSERIGAPTAEEISQLLDSVETNEEENDEELEKAMGVNS 650
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 2.1
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -3
Query: 404 MVNEAEKYRNEDDKQKETIQAKNALESYCFSMKST 300
M+++ RN+ +KQ TI KNA++ Y S+K T
Sbjct: 247 MLSKLVSSRNKPNKQ--TILTKNAIQDYLVSLKIT 279
>SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 26.6 bits (56), Expect = 2.7
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -3
Query: 500 LNVSAIXKSTNKEXKITITNDKGRLSKEEIERMVNEAEKYRNED 369
L VS +S K + + K EE+E VNE E++R D
Sbjct: 257 LCVSPEKRSLAKTREAVLAVTKWTTDDEELESFVNEEEEFRASD 300
>SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 827
Score = 26.6 bits (56), Expect = 2.7
Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Frame = -3
Query: 428 LSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEK---ISDSD 258
++K + + +AEK R ED+++K Q ++ L+S+ K +K + ISD D
Sbjct: 213 IAKSKHYKAERQAEKERYEDEREKLDEQMED-LQSFLSDYKKASRKSGIKTQRPIISDGD 271
Query: 257 KR 252
R
Sbjct: 272 AR 273
>SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 905
Score = 26.2 bits (55), Expect = 3.6
Identities = 16/66 (24%), Positives = 31/66 (46%)
Frame = -3
Query: 449 ITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLKEKI 270
+ +K SKE ++ EAE+ E +E + + + S K +++ LK K
Sbjct: 433 LEREKDEASKERLKAARKEAEQVEEETRPIREKYELEKSRGSELQDAKRRLDE--LKAKA 490
Query: 269 SDSDKR 252
D+++R
Sbjct: 491 EDAERR 496
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 3.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 113 EESPEVCRASRAEHPEPE 60
EE+P AS EHPEP+
Sbjct: 244 EEAPAAAAASENEHPEPK 261
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 3.6
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = -3
Query: 425 SKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKS 303
S+ E +++ E ++ ED+KQ E + K AL+ +C +++
Sbjct: 299 SETEQQQLFFEYKQKLLEDEKQLEKDRRKEALDDFCSLLRN 339
>SPBC15D4.01c ||SPBC2D10.21c|kinesin-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 633
Score = 25.8 bits (54), Expect = 4.8
Identities = 14/49 (28%), Positives = 31/49 (63%), Gaps = 1/49 (2%)
Frame = -3
Query: 419 EEIERMVNEAEKYRNEDDKQKETI-QAKNALESYCFSMKSTMEDEKLKE 276
+++E+M +K ++E+ ++ ETI Q + +E ++S +E+E +KE
Sbjct: 491 QKLEKMGGWMKKLQDENSEKTETIAQLEQIIEELHEELRS-LEEESIKE 538
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 25.8 bits (54), Expect = 4.8
Identities = 15/60 (25%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Frame = -3
Query: 443 NDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNA-LESYCFSMKSTMEDEKLKEKIS 267
+DK + E + E YRNE +E++ +N L S +++ +E+ K ++++S
Sbjct: 635 DDKMKSIDESTRELEKNYEVYRNEMTAIQESLSKRNQDLLSEMEAIRKELENSKYQQQLS 694
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 25.8 bits (54), Expect = 4.8
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = -3
Query: 437 KGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALE 327
+G L E E NEA++ NE+ E +N E
Sbjct: 89 EGELKLENFENQENEADEAENEETSYSEQNHTENTEE 125
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 25.8 bits (54), Expect = 4.8
Identities = 17/56 (30%), Positives = 32/56 (57%), Gaps = 1/56 (1%)
Frame = -3
Query: 458 KITITNDKGRLSKEEIERM-VNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTME 294
++TIT++ S E++E++ N AEK + E + K + ++ LES S + +E
Sbjct: 290 QMTITDE----SSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLE 341
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 25.8 bits (54), Expect = 4.8
Identities = 14/50 (28%), Positives = 24/50 (48%)
Frame = -3
Query: 437 KGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDE 288
+GR + + N+ E + DDKQ + KN+LE+ M+ + E
Sbjct: 1259 RGRQKTSIVAKYRNKRELPEDSDDKQDTASKDKNSLETIDEKMEDASKIE 1308
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.0 bits (52), Expect = 8.4
Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 6/74 (8%)
Frame = -3
Query: 572 PGIPPAPRGVPQ---I*GHLXHRCXG---ILNVSAIXKSTNKEXKITITNDKGRLSKEEI 411
P AP+ V Q + GH H + N S KS+NKE + + RLS
Sbjct: 382 PDPKAAPKAVSQKTNVDGHRNHEAKHGNTVQNESKSQKSSNKEGRSSRGGFFSRLSFSRS 441
Query: 410 ERMVNEAEKYRNED 369
+ + K ++ED
Sbjct: 442 SSRMKKGSKAKHED 455
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.0 bits (52), Expect = 8.4
Identities = 15/38 (39%), Positives = 17/38 (44%), Gaps = 2/38 (5%)
Frame = -3
Query: 119 VPEESPEVCRA--SRAEHPEPEVPPPGLEALAPPSRRS 12
V +SP R SR P E P A PPSRR+
Sbjct: 891 VSRDSPRYSRGGYSRGSVPPRETLAPSKGAYVPPSRRN 928
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,977,017
Number of Sequences: 5004
Number of extensions: 35983
Number of successful extensions: 185
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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