SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0327.Seq
         (421 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc...    51   8e-08
SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch...    50   1e-07
SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb...    26   2.1  
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy...    26   2.7  
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...    26   2.7  
SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc...    25   4.8  
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo...    25   6.3  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    25   6.3  
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo...    25   6.3  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    25   6.3  
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|...    24   8.4  
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ...    24   8.4  
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L...    24   8.4  
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb...    24   8.4  

>SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein
           L36|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 99

 Score = 50.8 bits (116), Expect = 8e-08
 Identities = 23/48 (47%), Positives = 33/48 (68%)
 Frame = +3

Query: 111 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLK 254
           K +T + +  RP+R KG  +K + FVR +VREV G A YE+R MEL++
Sbjct: 13  KVLTKRQLPERPSRRKGQLSKRTSFVRSIVREVAGFAPYERRVMELIR 60



 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 21/37 (56%), Positives = 26/37 (70%)
 Frame = +2

Query: 257 SKDKRALKFLKRRLGTHIRAKRKREELSNVLAQMRKA 367
           S+DKRA K  K+RLGT  RAK K EEL++V+   R A
Sbjct: 62  SQDKRARKLAKKRLGTLKRAKGKIEELTSVIQSSRLA 98


>SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein
           L36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 99

 Score = 50.4 bits (115), Expect = 1e-07
 Identities = 23/48 (47%), Positives = 33/48 (68%)
 Frame = +3

Query: 111 KGITDKAIRIRPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAMELLK 254
           K +T + +  RP+R KG  +K + FVR +VREV G A YE+R MEL++
Sbjct: 13  KTLTKRQLPERPSRRKGHLSKRTAFVRSIVREVAGFAPYERRVMELIR 60



 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 21/37 (56%), Positives = 26/37 (70%)
 Frame = +2

Query: 257 SKDKRALKFLKRRLGTHIRAKRKREELSNVLAQMRKA 367
           S+DKRA K  K+RLGT  RAK K EEL++V+   R A
Sbjct: 62  SQDKRARKLAKKRLGTLKRAKGKIEELTSVIQSSRLA 98


>SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 990

 Score = 26.2 bits (55), Expect = 2.1
 Identities = 16/44 (36%), Positives = 26/44 (59%)
 Frame = +2

Query: 197  STRSCRTRSI*EEGYGVT*VSKDKRALKFLKRRLGTHIRAKRKR 328
            +TR+C T+S+ EEG+    VS + +  +FL   L   I+ K K+
Sbjct: 928  ATRACLTQSLEEEGFPSRNVSNEIKR-RFLTDLLKARIKDKVKQ 970


>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 794

 Score = 25.8 bits (54), Expect = 2.7
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +1

Query: 310 PRQEEA*RT*QRARSDEEGSRQAHHHHH 393
           P Q+ A ++ +  +  + GS   HHHHH
Sbjct: 720 PGQQNANQS-EEKQGGQNGSNGGHHHHH 746


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score = 25.8 bits (54), Expect = 2.7
 Identities = 18/50 (36%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
 Frame = +2

Query: 254 VSKDKRALKFLKRRLGTHIRAKR--KREELSNVLAQMRKAAARLITITIK 397
           V+++ +ALK   R     +   +  KRE+ SNV  +M++ AARL+ I  K
Sbjct: 49  VAEEVQALKAKDRNFKPALAIVQVGKRED-SNVYVRMKEKAARLVGIDFK 97


>SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly
           scaffold protein Isu1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 192

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +3

Query: 141 RPARLKGLQTKHSKFVRDLVREVVGHAQYEKRAME 245
           RPA L+ L+T  SKFV  +    V    Y K  ++
Sbjct: 27  RPANLQFLKTNSSKFVPQVTAN-VSRRMYHKNVLD 60


>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 9/26 (34%), Positives = 17/26 (65%)
 Frame = -1

Query: 340 AKFFTLPLGADVCAQSSLQELQSTLV 263
           A+FFT PL ++VCA + +   +  ++
Sbjct: 560 AQFFTSPLVSNVCAYADVNHAKPVVI 585


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1101

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -3

Query: 119 DTLAASGYFSCFVAFSQAYCDFKTRSHDFG 30
           D   A  + S F AFSQ Y  +  +S+DFG
Sbjct: 319 DLRLACCFESHFFAFSQHYPRYLEQSNDFG 348


>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1183

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 9/24 (37%), Positives = 19/24 (79%)
 Frame = +2

Query: 287 KRRLGTHIRAKRKREELSNVLAQM 358
           K+RL  +I  K K+EE+++++A++
Sbjct: 89  KKRLDKYIENKLKKEEVASLIAKL 112


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 11/36 (30%), Positives = 17/36 (47%)
 Frame = +3

Query: 162  LQTKHSKFVRDLVREVVGHAQYEKRAMELLKCQKTS 269
            +Q  HSKF  +L +EV+    Y    +  + C   S
Sbjct: 1038 IQGNHSKFTTNLQKEVIISPHYFAHCLSEVVCNDNS 1073


>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
           ribosomal protein L8|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 253

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 296 LGTHIRAKRKREELSNVLAQMRKAAARLITI 388
           +G  IRA+RK   +     ++RK AA+L T+
Sbjct: 1   MGRVIRAQRKSGGIFQAHTRLRKGAAQLRTL 31


>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
           L8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 253

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 296 LGTHIRAKRKREELSNVLAQMRKAAARLITI 388
           +G  IRA+RK   +     ++RK AA+L T+
Sbjct: 1   MGRVIRAQRKSGGIFQAHTRLRKGAAQLRTL 31


>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
           L8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 253

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 296 LGTHIRAKRKREELSNVLAQMRKAAARLITI 388
           +G  IRA+RK   +     ++RK AA+L T+
Sbjct: 1   MGRVIRAQRKSGGIFQAHTRLRKGAAQLRTL 31


>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 420

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 7/14 (50%), Positives = 10/14 (71%)
 Frame = +1

Query: 352 SDEEGSRQAHHHHH 393
           SD++   + HHHHH
Sbjct: 290 SDKDLHHEGHHHHH 303


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,574,593
Number of Sequences: 5004
Number of extensions: 27176
Number of successful extensions: 78
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 72
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -