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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0323.Seq
         (548 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related s...    33   0.095
At3g06670.1 68416.m00786 expressed protein                             28   3.6  
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl...    28   3.6  
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl...    28   3.6  
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit...    27   6.2  
At2g20790.1 68415.m02445 expressed protein weak similarity to cl...    27   8.3  

>At5g15170.1 68418.m01777 tyrosyl-DNA phosphodiesterase-related
           similar to Chain A, Crystal Structure Of Human
           Tyrosyl-Dna Phosphodiesterase (Tdp1) [Homo sapiens]
           GI:20150581
          Length = 605

 Score = 33.5 bits (73), Expect = 0.095
 Identities = 22/78 (28%), Positives = 32/78 (41%), Gaps = 2/78 (2%)
 Frame = -3

Query: 504 IPRKTXMRDWIFSCRSASMAGVASTSSSXRDLLPRIASQIQ--WHPSADVAE*QSSPRNR 331
           +P     +  +FSC + S   V       +D + + +  +   W    D+ E  S P   
Sbjct: 515 LPSPIKTQGCVFSC-TESNPSVMKAKQETKDEVEKRSKLVTMTWQGDRDLPEIISLPVPY 573

Query: 330 QLRPKV*GPLDSPWLWAR 277
           QL PK   P D PW W R
Sbjct: 574 QLPPKPYSPEDVPWSWDR 591


>At3g06670.1 68416.m00786 expressed protein
          Length = 865

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 19/52 (36%), Positives = 30/52 (57%), Gaps = 2/52 (3%)
 Frame = +1

Query: 82  DEDVFRSVMGVLKTCSD-DNV-ALCLKEKALRYVENVSNSRELNLIDGVSLI 231
           D D FR++MGV K C D +NV  L +    ++ +  +++S+ L  I G  LI
Sbjct: 167 DHDFFRNLMGVFKICEDLENVDGLHMIFNIVKGIILLNSSQILEKIFGDELI 218


>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 490

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = +1

Query: 109 GVLKTCSDDNVALCLKEKALRYVENVSNSRELN-LIDGVSL 228
           GV+  C DDN ++ + E  LR+ E   ++ E + +ID V +
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 199


>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
           4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
           4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
          Length = 561

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
 Frame = +1

Query: 109 GVLKTCSDDNVALCLKEKALRYVENVSNSRELN-LIDGVSL 228
           GV+  C DDN ++ + E  LR+ E   ++ E + +ID V +
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 199


>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
           family protein low similarity to extensin [Volvox
           carteri] GI:21992
          Length = 312

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 12/17 (70%), Positives = 13/17 (76%)
 Frame = +1

Query: 241 SPRSAGPSSLYPTSPEP 291
           SP SA PSSL P+SP P
Sbjct: 44  SPSSAPPSSLSPSSPPP 60



 Score = 27.5 bits (58), Expect = 6.2
 Identities = 12/17 (70%), Positives = 13/17 (76%)
 Frame = +1

Query: 241 SPRSAGPSSLYPTSPEP 291
           SP SA PSSL P+SP P
Sbjct: 93  SPSSAPPSSLSPSSPPP 109


>At2g20790.1 68415.m02445 expressed protein weak similarity to
           clathrin-adaptor medium chain apm 3 (GI:12000359)
           [Dictyostelium discoideum]; weak similarity to
           Adapter-related protein complex 3 mu 2 subunit (Clathrin
           coat assembly protein AP47 homolog 2) (Golgi adaptor
           AP-1 47 kDa protein homolog 2) (HA1 47 kDa subunit
           homolog 2) (P47B) (Swiss-Prot:P53678) [Rattus
           norvegicus]
          Length = 613

 Score = 27.1 bits (57), Expect = 8.3
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +2

Query: 236 KAPLDQQVLRAFTRRAQSQGESSG 307
           + P DQQ+  +FTRR + +G + G
Sbjct: 49  RLPTDQQISDSFTRRKRREGSTRG 72


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,751,435
Number of Sequences: 28952
Number of extensions: 215826
Number of successful extensions: 691
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 691
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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