Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0317.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 100 6e-22
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 98 4e-21
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 92 2e-19
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 0.77
At1g30750.1 68414.m03758 expressed protein 29 3.1
At5g10520.1 68418.m01218 protein kinase family protein contains ... 28 4.1
At2g06000.2 68415.m00654 pentatricopeptide (PPR) repeat-containi... 27 7.2
At2g06000.1 68415.m00653 pentatricopeptide (PPR) repeat-containi... 27 7.2
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 27 9.5
At1g49400.1 68414.m05537 ribosomal protein S17 family protein si... 27 9.5
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 100 bits (240), Expect = 6e-22
Identities = 45/67 (67%), Positives = 58/67 (86%)
Frame = +1
Query: 55 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 234
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKGKK 67
Query: 235 RKETVCM 255
RK+TVC+
Sbjct: 68 RKDTVCI 74
Score = 94.7 bits (225), Expect = 4e-20
Identities = 44/94 (46%), Positives = 57/94 (60%)
Frame = +3
Query: 258 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSS*GVLLAIY 437
L+D+ C + KIRM DV+S+ CP VKYGKRVHILP+DD+ GV ++
Sbjct: 76 LADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLF 135
Query: 438 SKYT*SPYFMEAYRPIHRDDTFMVGGACAPVEFK 539
Y PYF+EAYRP+ + D F+V G VEFK
Sbjct: 136 DAYL-KPYFLEAYRPVRKGDLFLVRGGMRSVEFK 168
Score = 33.5 bits (73), Expect = 0.11
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +1
Query: 514 GHARPSSSKVVETDPSPFCIVASDTGI 594
G R KV+ETDP+ +C+VA DT I
Sbjct: 160 GGMRSVEFKVIETDPAEYCVVAPDTEI 186
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 97.9 bits (233), Expect = 4e-21
Identities = 43/67 (64%), Positives = 57/67 (85%)
Frame = +1
Query: 55 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKR 234
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK+
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKGKK 67
Query: 235 RKETVCM 255
RK+TVC+
Sbjct: 68 RKDTVCI 74
Score = 94.7 bits (225), Expect = 4e-20
Identities = 44/94 (46%), Positives = 57/94 (60%)
Frame = +3
Query: 258 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSS*GVLLAIY 437
L+D+ C + KIRM DV+S+ CP VKYGKRVHILP+DD+ GV ++
Sbjct: 76 LADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHILPVDDTVEGVTGNLF 135
Query: 438 SKYT*SPYFMEAYRPIHRDDTFMVGGACAPVEFK 539
Y PYF+EAYRP+ + D F+V G VEFK
Sbjct: 136 DAYL-KPYFLEAYRPVRKGDLFLVRGGMRSVEFK 168
Score = 33.5 bits (73), Expect = 0.11
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +1
Query: 514 GHARPSSSKVVETDPSPFCIVASDTGI 594
G R KV+ETDP+ +C+VA DT I
Sbjct: 160 GGMRSVEFKVIETDPAEYCVVAPDTEI 186
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 92.3 bits (219), Expect = 2e-19
Identities = 43/94 (45%), Positives = 56/94 (59%)
Frame = +3
Query: 258 LSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHILPIDDSS*GVLLAIY 437
L+D+ C + KIRM DV+S+ CP VKYG RVHILP+DD+ GV I+
Sbjct: 77 LADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHILPLDDTIEGVSGNIF 136
Query: 438 SKYT*SPYFMEAYRPIHRDDTFMVGGACAPVEFK 539
Y PYF+EAYRP+ + D F+V G +EFK
Sbjct: 137 DAYL-KPYFLEAYRPVRKGDLFLVRGGMRSIEFK 169
Score = 88.2 bits (209), Expect = 4e-18
Identities = 41/68 (60%), Positives = 56/68 (82%), Gaps = 1/68 (1%)
Frame = +1
Query: 55 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGK 231
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KGK
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKGK 67
Query: 232 RRKETVCM 255
+RK+TVC+
Sbjct: 68 KRKDTVCI 75
Score = 33.5 bits (73), Expect = 0.11
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +1
Query: 514 GHARPSSSKVVETDPSPFCIVASDTGI 594
G R KV+ETDP+ +C+VA DT I
Sbjct: 161 GGMRSIEFKVIETDPAEYCVVAPDTEI 187
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 0.77
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -2
Query: 207 TTEELKLLHFGL*KCHD*VVIAD 139
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At1g30750.1 68414.m03758 expressed protein
Length = 212
Score = 28.7 bits (61), Expect = 3.1
Identities = 13/53 (24%), Positives = 25/53 (47%)
Frame = +2
Query: 92 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSKANAARKPF 250
SS +P + S++ F++P + FN + + S S ++A +PF
Sbjct: 115 SSTGLNNPILGQPSTSSSLNQPLTGFNQPSSTGFNQPISSSSSSSSSAQNQPF 167
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 28.3 bits (60), Expect = 4.1
Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +2
Query: 50 KWQIIKVLMIYR-PRSSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNF 193
++ +I +L Y R ++ R P L+ +SA T +++ +P W NF
Sbjct: 95 RFSVIPLLASYELTRKNLRRKQP--KLTPSESAFTCEAFFMAKPSWRNF 141
>At2g06000.2 68415.m00654 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 536
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +2
Query: 497 HLHGRGGMRARRVQKWSKQIHHHFASWLL 583
H H GG +AR +Q ++++ H +WL+
Sbjct: 14 HTHSHGGAQARPLQNNTREVIHCPEAWLV 42
>At2g06000.1 68415.m00653 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 536
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +2
Query: 497 HLHGRGGMRARRVQKWSKQIHHHFASWLL 583
H H GG +AR +Q ++++ H +WL+
Sbjct: 14 HTHSHGGAQARPLQNNTREVIHCPEAWLV 42
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = -3
Query: 362 RTRSYGYHIRKTHTKVVSHDTVHPNFLIRAII 267
RT S +++ HTK++ H+ + L+R +I
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLI 59
>At1g49400.1 68414.m05537 ribosomal protein S17 family protein
similar to 40S ribosomal protein S17 GI:1620985 from
[Nicotiana plumbaginifolia]
Length = 116
Score = 27.1 bits (57), Expect = 9.5
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +2
Query: 29 SNNKF*IKWQIIKVLMIYRPRSSVARTDPTVSLSKKQSAMTTQS 160
S NK I +IIK IY P+++ A + S S SA TT S
Sbjct: 64 SKNKHWIVAEIIKKARIYSPKAAAAAVSASASAS---SASTTDS 104
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,992,283
Number of Sequences: 28952
Number of extensions: 292819
Number of successful extensions: 811
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 784
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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