BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0313.Seq (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 29 0.54 SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 28 0.94 SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 28 1.2 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 26 5.0 SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 25 6.6 SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces p... 25 8.8 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 25 8.8 >SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1131 Score = 29.1 bits (62), Expect = 0.54 Identities = 26/100 (26%), Positives = 44/100 (44%) Frame = +2 Query: 293 TVSKPSKFFSKISVKSPALLTSGT*SRESLNVNSIGTASFKYLDSV*VKIYGSVAGSRSL 472 T S S+ S + S +GT S NVNS+G +S + + I GS + + Sbjct: 610 TTSSTSEIVSTPASNSNTGSLNGT---SSFNVNSVGPSSSQTTPTSSSSITGSQSLKETS 666 Query: 473 LKATLRSSLSFNXSPMTTEIHLSK*NWKDLDAQEFSISAY 592 A + S++S+ S + + + D+Q FS + Y Sbjct: 667 SPAYVSSTVSYTSSSVDSSSTYNSTGSSSSDSQSFSGTTY 706 >SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1588 Score = 28.3 bits (60), Expect = 0.94 Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = -1 Query: 239 FEQPTEEQAESVVAEPDFIFNKPYFFLILNK 147 + +PT E+A++V+AE + + NKP + + K Sbjct: 1074 YSRPTIEEAKTVLAESENLTNKPEEYTVAQK 1104 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 27.9 bits (59), Expect = 1.2 Identities = 11/39 (28%), Positives = 25/39 (64%) Frame = -3 Query: 582 IENSWASRSFQFYFERWISVVIGXLLNDNEDLSVAFNKL 466 ++ S + + F+ + R +V++ ++NDNE+++V KL Sbjct: 121 LKKSGSYKGFEAFVNRTFAVLLRIVVNDNEEMAVLALKL 159 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.8 bits (54), Expect = 5.0 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = +2 Query: 209 FQLALLWAVRSQYAYAQHMGYIS 277 F L + Q++Y+ HMGYIS Sbjct: 207 FTLHCAVLIMKQFSYSHHMGYIS 229 >SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1822 Score = 25.4 bits (53), Expect = 6.6 Identities = 12/50 (24%), Positives = 31/50 (62%) Frame = +1 Query: 274 ILIYCIDRIEAVEVFLKNLCQESGPFNFRYIIT*ISQCQFDRNCELQVFR 423 +LI CI+++ ++V +KN ++ + ++ ++CQFD+ +++F+ Sbjct: 143 VLIKCIEQLIQIKVCMKNEKMKNLMEQLKPLLQ--TECQFDKFLIVELFQ 190 >SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 25.0 bits (52), Expect = 8.8 Identities = 10/31 (32%), Positives = 17/31 (54%) Frame = -3 Query: 396 IELTLRDSRDYVPEVKRAGLLTEIFEKNFDG 304 + LT+ +V V +AG+L +E +DG Sbjct: 155 LSLTVAQRHPFVDTVTKAGILLNYWENAYDG 185 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 25.0 bits (52), Expect = 8.8 Identities = 20/80 (25%), Positives = 33/80 (41%), Gaps = 2/80 (2%) Frame = -3 Query: 594 KYAEIENSWASRSFQFY--FERWISVVIGXLLNDNEDLSVAFNKLRDPATLPYILTQTES 421 KY+E+ ++ Q + + S + G + E+ +F K AT PYI + T Sbjct: 22 KYSELPIAYQEIPLQSLPPYPKVASKLKGVVAGGKENNIASFQKPSSKATRPYIPSYTRL 81 Query: 420 KYLKLAVPIELTLRDSRDYV 361 Y +PI S D + Sbjct: 82 TYSVPPLPIPPPSEQSLDTI 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,411,335 Number of Sequences: 5004 Number of extensions: 48858 Number of successful extensions: 139 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 135 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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