BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0309.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 27 1.8
SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1||... 26 3.2
SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid... 26 3.2
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 3.2
SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr 2|... 26 3.2
SPAC186.09 |||pyruvate decarboxylase |Schizosaccharomyces pombe|... 26 4.2
SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|ch... 25 5.6
SPBC1347.10 |cdc23|mcm10|MCM-associated protein Mcm10|Schizosacc... 25 7.3
SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 25 7.3
SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.7
>SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 785
Score = 27.1 bits (57), Expect = 1.8
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -2
Query: 307 YEGQLWNVYCSKQLPSTTEPFS*ACARCCDTITQREKIFFFY 182
Y WN + S+ P T FS C CC T+++ + FF +
Sbjct: 439 YIAPYWN-FPSEPFPRTMS-FSKMCPLCCTTLSKFVRHFFMF 478
>SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 467
Score = 26.2 bits (55), Expect = 3.2
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +2
Query: 410 SYYINTKSKSIVDLSKGERWKGG 478
SY++ SI+ S G++WK G
Sbjct: 56 SYFVTRNKSSIIAFSIGKKWKPG 78
>SPCC553.04 |cyp9||WD repeat containing cyclophilin family
peptidyl-prolyl cis-trans isomerase
Cyp9|Schizosaccharomyces pombe|chr 3|||Manual
Length = 610
Score = 26.2 bits (55), Expect = 3.2
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = -1
Query: 512 PSLDALVSILNNPLSISRLSRDPQYFCFSYLYSNFNSF 399
PSLD + S NNPL +DP F ++ F F
Sbjct: 382 PSLDVIAS--NNPLVEESFQKDPTLFATAWKKQRFYLF 417
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 26.2 bits (55), Expect = 3.2
Identities = 20/42 (47%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +1
Query: 40 SKTLIDLEKKKLYDIN*L---SLEKANNSSHWLQNEELKLHL 156
SKTL DL+K+KL + N + SL K N S LQ E+L L
Sbjct: 152 SKTLTDLKKRKLVERNKIMYFSLRKGPNFS--LQIEKLNTDL 191
>SPBC31E1.04 |pep12||SNARE Pep12|Schizosaccharomyces pombe|chr
2|||Manual
Length = 317
Score = 26.2 bits (55), Expect = 3.2
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = -1
Query: 437 FCFSYLYSNFNSFRLQHA**NNLGSTR 357
FCF ++ F+SFR Q+A NL S R
Sbjct: 243 FCFLKSFAMFSSFRSQNANLYNLNSIR 269
>SPAC186.09 |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 572
Score = 25.8 bits (54), Expect = 4.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 152 ILDIKIKFSLIKKKDLFPLCDSVTAPRACSAEG 250
++D IK +L+KKK ++ + A + C+A G
Sbjct: 158 LIDYAIKMALLKKKPVYIEVPTNVASQPCAAPG 190
>SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 397
Score = 25.4 bits (53), Expect = 5.6
Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 6/55 (10%)
Frame = +1
Query: 58 LEKKKLYDIN*LSLEKANNSSHWLQNEELKL------HLRY*NQVQPYKKKRSFP 204
LEK D N L L++ N L EL+L +L Y N + PY+ S+P
Sbjct: 115 LEKVSTADNN-LVLQELENLRERLNQVELQLSERPSSYLGYHNNLSPYRSPNSYP 168
>SPBC1347.10 |cdc23|mcm10|MCM-associated protein
Mcm10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 593
Score = 25.0 bits (52), Expect = 7.3
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -3
Query: 303 KGSSGMFIAVNSYPQRPSPSAEHARGAVTLSHKGKRS 193
KG +G +++ P+ P P H R A + + KRS
Sbjct: 80 KGKTGKDVSLGKGPRGPLPKPFHERLAEARNQERKRS 116
>SPAC8E11.01c
||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 25.0 bits (52), Expect = 7.3
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 110 TTHHTGYKTKN*NYILDIKIKFSLIKKKDLFPLCDS 217
TTH+ +T+N Y LD I F KK + + +S
Sbjct: 111 TTHYDNRETQNIAYSLDGGITFIKYKKNPILDIKES 146
>SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 24.6 bits (51), Expect = 9.7
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -2
Query: 337 VFTFPVFFLDYEGQLWNVYCSKQLPSTTE 251
+ TFP D + QLWNV L S ++
Sbjct: 72 ILTFPFLDPDSQNQLWNVNFRNLLKSLSK 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,063,699
Number of Sequences: 5004
Number of extensions: 39338
Number of successful extensions: 84
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 84
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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