BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0309.Seq (548 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g31450.1 68414.m03851 aspartyl protease family protein contai... 29 1.5 At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 28 3.6 At1g66430.1 68414.m07546 pfkB-type carbohydrate kinase family pr... 28 3.6 >At1g31450.1 68414.m03851 aspartyl protease family protein contains eukaryotic and viral aspartyl proteases active site, PROSITE:PS00141 Length = 445 Score = 29.5 bits (63), Expect = 1.5 Identities = 15/52 (28%), Positives = 26/52 (50%) Frame = -3 Query: 309 TTKGSSGMFIAVNSYPQRPSPSAEHARGAVTLSHKGKRSFFFIRLNLILISK 154 TT G+S + + NS P +PS + A L K +++F+ L + + K Sbjct: 249 TTNGTSVINLGTNSIPS--NPSKDSATLTTPLIQKDPETYYFLTLEAVTVGK 298 >At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein similar to Anther-specific proline-rich protein APG from Brassica napus (SP|P40603 ), Arabidopsis thaliana (GI:22599); contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif Length = 367 Score = 28.3 bits (60), Expect = 3.6 Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 1/51 (1%) Frame = -1 Query: 542 AEAEDRGQLSPSLDALVSILNNPLSISRLSRDPQYFC-FSYLYSNFNSFRL 393 A+ RG + +L+ VSI N L S LSR Q F S+LYS++ ++ L Sbjct: 238 AKTVPRGSCNDALNFAVSIFNTKLRKS-LSRMTQKFIKTSFLYSDYYNYML 287 >At1g66430.1 68414.m07546 pfkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 pfkB family carbohydrate kinase Length = 384 Score = 28.3 bits (60), Expect = 3.6 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 276 VNSYPQRPSPSAEHARGAVTLSHKGKRSFFFIR 178 VN+ R P A A VTL+++G+R F F R Sbjct: 137 VNNDGMRFDPGARTALAFVTLTNEGEREFMFYR 169 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,448,961 Number of Sequences: 28952 Number of extensions: 194796 Number of successful extensions: 493 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 492 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 493 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1033331880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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