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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0309.Seq
         (548 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g31450.1 68414.m03851 aspartyl protease family protein contai...    29   1.5  
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot...    28   3.6  
At1g66430.1 68414.m07546 pfkB-type carbohydrate kinase family pr...    28   3.6  

>At1g31450.1 68414.m03851 aspartyl protease family protein contains
           eukaryotic and viral aspartyl proteases active site,
           PROSITE:PS00141
          Length = 445

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 15/52 (28%), Positives = 26/52 (50%)
 Frame = -3

Query: 309 TTKGSSGMFIAVNSYPQRPSPSAEHARGAVTLSHKGKRSFFFIRLNLILISK 154
           TT G+S + +  NS P   +PS + A     L  K   +++F+ L  + + K
Sbjct: 249 TTNGTSVINLGTNSIPS--NPSKDSATLTTPLIQKDPETYYFLTLEAVTVGK 298


>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
           similar to Anther-specific proline-rich protein APG from
           Brassica napus (SP|P40603 ), Arabidopsis thaliana
           (GI:22599); contains Pfam profile PF00657:
           Lipase/Acylhydrolase with GDSL-like motif
          Length = 367

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
 Frame = -1

Query: 542 AEAEDRGQLSPSLDALVSILNNPLSISRLSRDPQYFC-FSYLYSNFNSFRL 393
           A+   RG  + +L+  VSI N  L  S LSR  Q F   S+LYS++ ++ L
Sbjct: 238 AKTVPRGSCNDALNFAVSIFNTKLRKS-LSRMTQKFIKTSFLYSDYYNYML 287


>At1g66430.1 68414.m07546 pfkB-type carbohydrate kinase family
           protein contains Pfam profile: PF00294 pfkB family
           carbohydrate kinase
          Length = 384

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -3

Query: 276 VNSYPQRPSPSAEHARGAVTLSHKGKRSFFFIR 178
           VN+   R  P A  A   VTL+++G+R F F R
Sbjct: 137 VNNDGMRFDPGARTALAFVTLTNEGEREFMFYR 169


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,448,961
Number of Sequences: 28952
Number of extensions: 194796
Number of successful extensions: 493
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 493
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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