BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0305.Seq (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 1.6 SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|c... 27 1.6 SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit |Sch... 27 1.6 SPCC70.06 |||nuclear export factor|Schizosaccharomyces pombe|chr... 25 6.3 SPAC2F3.02 |||ER protein translocation subcomplex subunit|Schizo... 25 6.3 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 25 6.3 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 8.4 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.1 bits (57), Expect = 1.6 Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = +3 Query: 9 PLAVPVTP-IQAQKVQDPVHPAVAALMDIAPAIVVEAYHPPAVPATLIQAQRMQDP 173 P A + P QA V V PAV+A + PA+V HP P I ++DP Sbjct: 1274 PTAQQIQPGKQASAVSSNV-PAVSASISTPPAVVPTVQHPQ--PTKQIPTAAVKDP 1326 >SPCC757.08 |||exosome subunit Rrp45 |Schizosaccharomyces pombe|chr 3|||Manual Length = 291 Score = 27.1 bits (57), Expect = 1.6 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 3/56 (5%) Frame = -3 Query: 203 IHKCCYRQMYWILHSLCL--NQCCWNCRWMIRF-HHDGWSYIHKCCYRRMYGILHF 45 I K R SLC+ Q CW+ R + F +HDG + + C + + HF Sbjct: 107 IEKAVRRSNALDTESLCIISGQKCWHVRASVHFINHDG-NLVDAACIAVIAALCHF 161 >SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 1006 Score = 27.1 bits (57), Expect = 1.6 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +3 Query: 141 TLIQAQRMQDPVHLAVAALMDIALAIVVEVYHPPAV 248 T+ +Q + HLA+A+ + A V+ VY P A+ Sbjct: 149 TVFASQSNVEAAHLALASTLAAKAAPVIHVYEPDAI 184 >SPCC70.06 |||nuclear export factor|Schizosaccharomyces pombe|chr 3|||Manual Length = 458 Score = 25.0 bits (52), Expect = 6.3 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 2/39 (5%) Frame = +3 Query: 21 PVTPIQAQKVQDPVHPAVA--ALMDIAPAIVVEAYHPPA 131 P ++ +K+Q +HP P++ V+AYH PA Sbjct: 79 PEFELEERKLQHAIHPYELDPVSKQAHPSLAVKAYHRPA 117 >SPAC2F3.02 |||ER protein translocation subcomplex subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 192 Score = 25.0 bits (52), Expect = 6.3 Identities = 8/17 (47%), Positives = 13/17 (76%) Frame = -3 Query: 71 RRMYGILHFLSLNRCYW 21 +++YG+ L+LNRC W Sbjct: 82 KKLYGLALQLALNRCTW 98 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 25.0 bits (52), Expect = 6.3 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -3 Query: 341 VHXDAWSYIRKCLLPP 294 V +AWS+I KCL P Sbjct: 434 VSDEAWSFITKCLTEP 449 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 24.6 bits (51), Expect = 8.4 Identities = 17/48 (35%), Positives = 21/48 (43%), Gaps = 7/48 (14%) Frame = +3 Query: 57 PVHPAVAALMDIAPAI-------VVEAYHPPAVPATLIQAQRMQDPVH 179 P P A++ APAI V E YHPP T + +Q P H Sbjct: 908 PPPPPTASMTASAPAIASPPPPKVGETYHPPTASGTRVPP--VQQPSH 953 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,665,584 Number of Sequences: 5004 Number of extensions: 31066 Number of successful extensions: 67 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 62 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 67 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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