Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0303.Seq
(612 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic... 122 7e-27
UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor... 114 2e-24
UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;... 89 6e-17
UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor... 70 4e-11
UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=... 66 6e-10
UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor... 65 1e-09
UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor... 64 3e-09
UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative; ... 58 1e-07
UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;... 47 4e-04
UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;... 46 0.001
UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release fa... 44 0.004
UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;... 41 0.020
UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-... 40 0.035
UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:... 38 0.14
UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus ... 38 0.14
UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2; Cr... 38 0.14
UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;... 37 0.33
UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1... 34 2.3
UniRef50_UPI00006CC094 Cluster: hypothetical protein TTHERM_0021... 33 5.3
UniRef50_Q8I368 Cluster: Putative uncharacterized protein PFI040... 33 5.3
UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing prot... 32 9.3
>UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic
release factor 1 CG5605-PA, isoform A; n=1; Apis
mellifera|Rep: PREDICTED: similar to eukaryotic release
factor 1 CG5605-PA, isoform A - Apis mellifera
Length = 265
Score = 122 bits (294), Expect = 7e-27
Identities = 69/93 (74%), Positives = 72/93 (77%), Gaps = 1/93 (1%)
Frame = +3
Query: 336 SEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-I 512
+EE+SADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS I
Sbjct: 3 NEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI 62
Query: 513 SSHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
S V +S GAI K PNGLV
Sbjct: 63 KSRVNRLS-VLGAITSVQHRLKLYTKVPPNGLV 94
>UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=137; Eukaryota|Rep: Eukaryotic peptide
chain release factor subunit 1 - Homo sapiens (Human)
Length = 437
Score = 114 bits (274), Expect = 2e-24
Identities = 65/92 (70%), Positives = 69/92 (75%), Gaps = 1/92 (1%)
Frame = +3
Query: 339 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-IS 515
+ S+ADRNVEIWKIKKLIKSLE ARGNGTSMISLIIPPKDQISRV+KMLADEFGTAS I
Sbjct: 4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIK 63
Query: 516 SHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
S V +S GAI K PNGLV
Sbjct: 64 SRVNRLS-VLGAITSVQQRLKLYNKVPPNGLV 94
>UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;
n=1; Encephalitozoon cuniculi|Rep: PEPTIDE CHAIN RELEASE
FACTOR SUBUNIT 1 - Encephalitozoon cuniculi
Length = 386
Score = 89.4 bits (212), Expect = 6e-17
Identities = 49/83 (59%), Positives = 60/83 (72%), Gaps = 1/83 (1%)
Frame = +3
Query: 363 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISSHV*IVSQ 539
VE+W++KKL+++L +RGNGTSMIS+IIPPKDQISRVSKML DE+GTAS I S V +S
Sbjct: 5 VEMWRMKKLLRNLRDSRGNGTSMISIIIPPKDQISRVSKMLTDEYGTASNIKSRVNRLS- 63
Query: 540 CFGAILXSXPTQSCILKXHPNGL 608
GAI + K PNGL
Sbjct: 64 VLGAITSAQSKLKQYSKTPPNGL 86
>UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=5; Eukaryota|Rep: Eukaryotic peptide chain
release factor subunit 1 - Loxodes striatus
Length = 436
Score = 70.1 bits (164), Expect = 4e-11
Identities = 36/80 (45%), Positives = 50/80 (62%)
Frame = +3
Query: 372 WKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASISSHV*IVSQCFGA 551
WKI++LI+ LE A+GNGTS+ISLIIPPK+QIS +++ L D G A + A
Sbjct: 12 WKIRRLIEYLEKAKGNGTSLISLIIPPKEQISLINQKLTDAHGRAQNIKSKAVQKAVQDA 71
Query: 552 ILXSXPTQSCILKXHPNGLV 611
I+ + S I + PNGL+
Sbjct: 72 IISTKQKLSVIKQIPPNGLI 91
>UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=1;
Guillardia theta|Rep: Eukaryotic release factor 1
homolog - Guillardia theta (Cryptomonas phi)
Length = 409
Score = 66.1 bits (154), Expect = 6e-10
Identities = 30/48 (62%), Positives = 37/48 (77%)
Frame = +3
Query: 366 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
E+WKIKK + L +GNGTSMISL+IP + IS+VSKML+DE TAS
Sbjct: 9 ELWKIKKTYQYLSTIKGNGTSMISLVIPSNEHISKVSKMLSDELATAS 56
Score = 32.3 bits (70), Expect = 9.3
Identities = 17/29 (58%), Positives = 18/29 (62%)
Frame = +2
Query: 509 NIKSRVNRLSVLWRNSXVXTDSKLYTKXP 595
NIKSR+NR SVL S V K YTK P
Sbjct: 57 NIKSRINRSSVLSAISSVQQKLKYYTKTP 85
>UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=2; Giardia intestinalis|Rep: Eukaryotic
peptide chain release factor subunit 1 - Giardia lamblia
(Giardia intestinalis)
Length = 457
Score = 64.9 bits (151), Expect = 1e-09
Identities = 36/86 (41%), Positives = 53/86 (61%), Gaps = 1/86 (1%)
Frame = +3
Query: 354 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISSHV*I 530
+R + IW++K+ I+ LE G+GTS+ISL+IPP +Q+S V+ ML +E+GTAS I S V
Sbjct: 15 ERQIAIWQLKRTIEKLESYTGSGTSVISLLIPPGEQLSSVTGMLTNEYGTASNIKSRV-N 73
Query: 531 VSQCFGAILXSXPTQSCILKXHPNGL 608
+ AI + + PNGL
Sbjct: 74 KNAVLSAITSAMSRLKLYNRLPPNGL 99
>UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=6; Trypanosomatidae|Rep: Eukaryotic peptide
chain release factor subunit 1 - Trypanosoma brucei
brucei
Length = 452
Score = 63.7 bits (148), Expect = 3e-09
Identities = 40/92 (43%), Positives = 57/92 (61%), Gaps = 1/92 (1%)
Frame = +3
Query: 339 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-IS 515
E S +++ +E +K+KKLI+ LE ARG GTS+IS+ + PK+QIS + L +E+GTAS I
Sbjct: 5 ELSESEKAIERYKVKKLIQMLESARGMGTSVISIYMTPKEQISGMVTKLNNEYGTASNIK 64
Query: 516 SHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
SH +S AI S + PNGL+
Sbjct: 65 SHTNKLS-VQSAITASLGRLKQYNRLPPNGLL 95
>UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative;
n=2; Trichomonas vaginalis|Rep: Eukaryotic release
factor 1, putative - Trichomonas vaginalis G3
Length = 438
Score = 58.4 bits (135), Expect = 1e-07
Identities = 25/49 (51%), Positives = 37/49 (75%)
Frame = +3
Query: 363 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
+E++KIK+LI L A G+GTSM++++IPP D + R+ + L DE GTAS
Sbjct: 14 IELFKIKRLINYLRDAHGDGTSMVTILIPPGDAVHRMRQKLTDELGTAS 62
>UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoplasmatales|Rep: Peptide chain release factor
subunit 1 - Thermoplasma volcanium
Length = 418
Score = 46.8 bits (106), Expect = 4e-04
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = +3
Query: 354 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
D + ++ K+ ++ L G GT +ISL IPP+ QIS V L DE+ T+S
Sbjct: 7 DEQIRRYEFKRALEELSKLHGRGTELISLYIPPEKQISDVVAYLRDEYSTSS 58
>UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;
n=5; Archaea|Rep: Peptide chain release factor subunit 1
- Pyrococcus furiosus
Length = 420
Score = 45.6 bits (103), Expect = 0.001
Identities = 20/50 (40%), Positives = 33/50 (66%)
Frame = +3
Query: 357 RNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 506
R+ +++++KK I L+ RG GT +ISL IP +S+V + L +E+ TA
Sbjct: 7 RDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTA 56
>UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release
factor 1; n=3; Paramecium tetraurelia|Rep: Eukaryotic
polypeptide chain release factor 1 - Paramecium
tetraurelia
Length = 437
Score = 43.6 bits (98), Expect = 0.004
Identities = 21/55 (38%), Positives = 36/55 (65%)
Frame = +3
Query: 345 SSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
+ A+ +E +++KKLIK+L R GTS++S+ IPPK IS ++ L ++ A+
Sbjct: 6 NDAEIALEQFRLKKLIKTLSQERTAGTSVVSVYIPPKRIISDITNRLNTQYAEAA 60
>UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;
n=12; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Methanococcus jannaschii
Length = 421
Score = 41.1 bits (92), Expect = 0.020
Identities = 19/48 (39%), Positives = 31/48 (64%)
Frame = +3
Query: 366 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
+++ KK++K L+ +G GT +ISL IP +IS V++ L +E AS
Sbjct: 8 QLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQAS 55
>UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-2;
n=9; Euryarchaeota|Rep: Peptide chain release factor
subunit 1-2 - Methanosarcina acetivorans
Length = 415
Score = 40.3 bits (90), Expect = 0.035
Identities = 21/48 (43%), Positives = 29/48 (60%)
Frame = +3
Query: 366 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
E + KK +++L+ G T +ISL IPP QIS V+K L +E AS
Sbjct: 8 EKYVFKKQLETLKSKNGRSTELISLYIPPDKQISDVTKHLREEHEQAS 55
>UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:
NEQ052 - Nanoarchaeum equitans
Length = 358
Score = 38.3 bits (85), Expect = 0.14
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = +3
Query: 366 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
E +K+K L++ L+ G GT ++SL IP I ++ L E+ TA+
Sbjct: 5 EKYKLKSLLRQLKSIEGRGTELVSLYIPSNYPIGKILDQLNYEYATAA 52
>UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: ERF1 domain 2 protein
- Candidatus Nitrosopumilus maritimus SCM1
Length = 426
Score = 38.3 bits (85), Expect = 0.14
Identities = 17/49 (34%), Positives = 29/49 (59%)
Frame = +3
Query: 360 NVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 506
+V+++KI+K ++ L G GT +I++ IP Q+ + L E GTA
Sbjct: 11 SVKVYKIRKTLEELSKKSGRGTELITVYIPKGKQLHEIIGTLQQEQGTA 59
>UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2;
Crenarchaeota|Rep: Peptide chain release factor 1 -
Cenarchaeum symbiosum
Length = 411
Score = 38.3 bits (85), Expect = 0.14
Identities = 17/46 (36%), Positives = 28/46 (60%)
Frame = +3
Query: 369 IWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 506
++KI+K++ L G GT +IS+ IP Q+ + +L +E GTA
Sbjct: 1 MYKIRKMLDELSGISGRGTELISVYIPKGKQLHEIISVLKEEQGTA 46
>UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;
n=7; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Halobacterium salinarium (Halobacterium
halobium)
Length = 416
Score = 37.1 bits (82), Expect = 0.33
Identities = 20/61 (32%), Positives = 34/61 (55%)
Frame = +3
Query: 327 NKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 506
++ E S DR ++ +K+I+ L+ G+GT ++++ IPP QIS V + E A
Sbjct: 2 SEQDEVPSEDRRK--YEFRKVIEELKDYEGSGTQLVTIYIPPDKQISDVVAHVTQEHSEA 59
Query: 507 S 509
S
Sbjct: 60 S 60
>UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1;
n=2; Methanosarcinaceae|Rep: Peptide chain release
factor, subunit 1 - Methanosarcina barkeri (strain
Fusaro / DSM 804)
Length = 415
Score = 34.3 bits (75), Expect = 2.3
Identities = 19/48 (39%), Positives = 26/48 (54%)
Frame = +3
Query: 366 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS 509
E + KK +++L G T +ISL IP QIS V+ L +E AS
Sbjct: 8 EKYVFKKKLETLRNKNGKSTELISLYIPSDKQISDVTNHLREEHEQAS 55
>UniRef50_UPI00006CC094 Cluster: hypothetical protein
TTHERM_00215930; n=1; Tetrahymena thermophila SB210|Rep:
hypothetical protein TTHERM_00215930 - Tetrahymena
thermophila SB210
Length = 905
Score = 33.1 bits (72), Expect = 5.3
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +3
Query: 375 KIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEF 497
K++ LI+ L NGT+ +SL I K Q +++S+ L DEF
Sbjct: 182 KVEYLIEQLLEGSNNGTNTLSLKILQKQQANQLSQDLKDEF 222
>UniRef50_Q8I368 Cluster: Putative uncharacterized protein PFI0400c;
n=2; Plasmodium|Rep: Putative uncharacterized protein
PFI0400c - Plasmodium falciparum (isolate 3D7)
Length = 352
Score = 33.1 bits (72), Expect = 5.3
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = -2
Query: 461 LILRWNDQGYHRCSISSGHFQTLYKFLYLPYFDISIGRRFFRHFVSLFI*LINHKHYI 288
+IL W+ Y +SS H QTLY FL + + I++ +FV + I + + I
Sbjct: 172 IILFWSQVYYASVLVSSPHLQTLYIFLNVLVYAINVILASLAYFVKAYFDYIYYNYII 229
>UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing
protein; n=1; Trichomonas vaginalis G3|Rep: DHHC zinc
finger domain containing protein - Trichomonas vaginalis
G3
Length = 347
Score = 32.3 bits (70), Expect = 9.3
Identities = 19/55 (34%), Positives = 26/55 (47%)
Frame = -2
Query: 380 YLPYFDISIGRRFFRHFVSLFI*LINHKHYICINN*NNVFAQTKTLNKRILWKGS 216
Y P+ + IG R R+F+ I I Y CIN+ VF + +RI W S
Sbjct: 172 YCPWVLVPIGERNHRYFLLFLIACIVASTYYCINDVFVVFFYFMQVYQRIPWTNS 226
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 584,856,157
Number of Sequences: 1657284
Number of extensions: 10989967
Number of successful extensions: 25842
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 25082
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25834
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 43977329078
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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