BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0303.Seq (612 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 104 4e-23 At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 100 7e-22 At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 97 6e-21 At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 29 1.8 At4g14480.1 68417.m02233 protein kinase family protein contains ... 29 3.2 At5g56240.1 68418.m07018 expressed protein 28 5.6 At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f... 28 5.6 At1g60280.1 68414.m06788 no apical meristem (NAM) protein-relate... 28 5.6 At3g58490.1 68416.m06519 phosphatidic acid phosphatase family pr... 27 9.8 >At5g47880.1 68418.m05915 eukaryotic peptide chain release factor subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic peptide chain release factor subunit 1-1 (eRF1-1) (Eukaryotic release factor 1-1) (Omnipotent suppressor protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis thaliana}, eukaryotic release factor 1 homolog GI:1155261 from [Arabidopsis thaliana]; contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 436 Score = 104 bits (250), Expect = 4e-23 Identities = 56/94 (59%), Positives = 68/94 (72%), Gaps = 1/94 (1%) Frame = +3 Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 509 M +++ D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS Sbjct: 1 MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60 Query: 510 ISSHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611 I S V S GAI + + PNGLV Sbjct: 61 IKSRVNRQS-VLGAITSAQQRLKLYNRVPPNGLV 93 >At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 435 Score = 100 bits (240), Expect = 7e-22 Identities = 54/91 (59%), Positives = 65/91 (71%), Gaps = 1/91 (1%) Frame = +3 Query: 342 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISS 518 + +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GTAS I S Sbjct: 3 DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62 Query: 519 HV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611 V S AI + K PNGLV Sbjct: 63 RVNRQS-VLSAITSAQQRLKLYNKVPPNGLV 92 >At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 434 Score = 97.5 bits (232), Expect = 6e-21 Identities = 57/94 (60%), Positives = 65/94 (69%), Gaps = 1/94 (1%) Frame = +3 Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 509 M+EE AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS Sbjct: 1 MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58 Query: 510 ISSHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611 I S V S AI + K NGLV Sbjct: 59 IKSRVNRQS-VLSAITSAQQRLKLYNKVPTNGLV 91 >At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains similarity to NAD kinase [Homo sapiens] gi|20070086|gb|AAM01195; contains Pfam domain, PF01513: ATP-NAD kinase Length = 985 Score = 29.5 bits (63), Expect = 1.8 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +3 Query: 393 KSLEMARGNGTSMISLIIPPKDQISR 470 KS+ RGNG S + +PP D +SR Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586 >At4g14480.1 68417.m02233 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 487 Score = 28.7 bits (61), Expect = 3.2 Identities = 16/54 (29%), Positives = 30/54 (55%) Frame = +3 Query: 324 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 485 +N+ E D+++ + K+K L KSLE R + +I + K++ SR ++L Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460 >At5g56240.1 68418.m07018 expressed protein Length = 986 Score = 27.9 bits (59), Expect = 5.6 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = +2 Query: 344 IVGRSKCRNMEDKETYKEFGNGQRKWNIDDIPDHST*GSNLESIKDVG 487 I G++ C+ ED+ E G G +D IP GS L S +D+G Sbjct: 668 IYGQTSCK--EDERFDSEVGQGSLFVEVDTIPIPGPPGSFLPSPRDMG 713 >At3g59610.1 68416.m06651 F-box family protein / jacalin lectin family protein contains Pfam profile PF01419: Jacalin-like lectin domain Length = 521 Score = 27.9 bits (59), Expect = 5.6 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +3 Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 467 + +E RN+EIW K+ IK+ + +++S+++P +S Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295 >At1g60280.1 68414.m06788 no apical meristem (NAM) protein-related contains Pfam PF02365: No apical meristem (NAM) protein; contains similarity to NAM-like protein GI:4337200 from [Arabidopsis thaliana] Length = 347 Score = 27.9 bits (59), Expect = 5.6 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +1 Query: 445 FHLRIKSREYQRCWLMNLVQLQYQVTCE 528 F L+ K REY+R W+M +L + C+ Sbjct: 128 FCLKKKPREYKRSWVMEEYRLNNNLNCK 155 >At3g58490.1 68416.m06519 phosphatidic acid phosphatase family protein / PAP2 family protein similar to sphingosine-1-phosphate phosphohydrolase from [Mus musculus] GI:9623190, [Homo sapiens] GI:23345324; contains Pfam profile PF01569: PAP2 superfamily Length = 416 Score = 27.1 bits (57), Expect = 9.8 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = -2 Query: 416 SSGHFQTLYKFLYLPYFDISIGRRFFRH 333 S G+ Q L +FL FDI G RFF++ Sbjct: 369 SVGYLQKLCEFLSHDSFDIDTGIRFFQY 396 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,997,578 Number of Sequences: 28952 Number of extensions: 257808 Number of successful extensions: 605 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 586 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 602 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1226538000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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