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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0303.Seq
         (612 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g47880.1 68418.m05915 eukaryotic peptide chain release factor...   104   4e-23
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot...   100   7e-22
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot...    97   6e-21
At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ...    29   1.8  
At4g14480.1 68417.m02233 protein kinase family protein contains ...    29   3.2  
At5g56240.1 68418.m07018 expressed protein                             28   5.6  
At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f...    28   5.6  
At1g60280.1 68414.m06788 no apical meristem (NAM) protein-relate...    28   5.6  
At3g58490.1 68416.m06519 phosphatidic acid phosphatase family pr...    27   9.8  

>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
           subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
           peptide chain release factor subunit 1-1 (eRF1-1)
           (Eukaryotic release factor 1-1) (Omnipotent suppressor
           protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
           thaliana}, eukaryotic release factor 1 homolog
           GI:1155261 from [Arabidopsis thaliana]; contains Pfam
           profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
           PF03465 eRF1 domain 3
          Length = 436

 Score =  104 bits (250), Expect = 4e-23
 Identities = 56/94 (59%), Positives = 68/94 (72%), Gaps = 1/94 (1%)
 Frame = +3

Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 509
           M +++  D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS 
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 510 ISSHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
           I S V   S   GAI  +        +  PNGLV
Sbjct: 61  IKSRVNRQS-VLGAITSAQQRLKLYNRVPPNGLV 93


>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 435

 Score =  100 bits (240), Expect = 7e-22
 Identities = 54/91 (59%), Positives = 65/91 (71%), Gaps = 1/91 (1%)
 Frame = +3

Query: 342 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS-ISS 518
           +  +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLADE+GTAS I S
Sbjct: 3   DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASNIKS 62

Query: 519 HV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
            V   S    AI  +        K  PNGLV
Sbjct: 63  RVNRQS-VLSAITSAQQRLKLYNKVPPNGLV 92


>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
           / eRF1 family protein contains Pfam profiles: PF03463
           eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
           domain 3
          Length = 434

 Score = 97.5 bits (232), Expect = 6e-21
 Identities = 57/94 (60%), Positives = 65/94 (69%), Gaps = 1/94 (1%)
 Frame = +3

Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTAS- 509
           M+EE  AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML DE+GTAS 
Sbjct: 1   MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 58

Query: 510 ISSHV*IVSQCFGAILXSXPTQSCILKXHPNGLV 611
           I S V   S    AI  +        K   NGLV
Sbjct: 59  IKSRVNRQS-VLSAITSAQQRLKLYNKVPTNGLV 91


>At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains
           similarity to NAD kinase [Homo sapiens]
           gi|20070086|gb|AAM01195; contains Pfam domain, PF01513:
           ATP-NAD kinase
          Length = 985

 Score = 29.5 bits (63), Expect = 1.8
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +3

Query: 393 KSLEMARGNGTSMISLIIPPKDQISR 470
           KS+   RGNG S   + +PP D +SR
Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586


>At4g14480.1 68417.m02233 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 487

 Score = 28.7 bits (61), Expect = 3.2
 Identities = 16/54 (29%), Positives = 30/54 (55%)
 Frame = +3

Query: 324 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 485
           +N+  E    D+++ + K+K L KSLE  R   + +I  +   K++ SR  ++L
Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460


>At5g56240.1 68418.m07018 expressed protein 
          Length = 986

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 17/48 (35%), Positives = 24/48 (50%)
 Frame = +2

Query: 344 IVGRSKCRNMEDKETYKEFGNGQRKWNIDDIPDHST*GSNLESIKDVG 487
           I G++ C+  ED+    E G G     +D IP     GS L S +D+G
Sbjct: 668 IYGQTSCK--EDERFDSEVGQGSLFVEVDTIPIPGPPGSFLPSPRDMG 713


>At3g59610.1 68416.m06651 F-box family protein / jacalin lectin
           family protein contains Pfam profile PF01419:
           Jacalin-like lectin domain
          Length = 521

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +3

Query: 333 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 467
           + +E    RN+EIW  K+ IK+ +       +++S+++P    +S
Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295


>At1g60280.1 68414.m06788 no apical meristem (NAM) protein-related
           contains Pfam PF02365: No apical meristem (NAM) protein;
           contains similarity to NAM-like protein GI:4337200 from
           [Arabidopsis thaliana]
          Length = 347

 Score = 27.9 bits (59), Expect = 5.6
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 445 FHLRIKSREYQRCWLMNLVQLQYQVTCE 528
           F L+ K REY+R W+M   +L   + C+
Sbjct: 128 FCLKKKPREYKRSWVMEEYRLNNNLNCK 155


>At3g58490.1 68416.m06519 phosphatidic acid phosphatase family
           protein / PAP2 family protein similar to
           sphingosine-1-phosphate phosphohydrolase from [Mus
           musculus] GI:9623190, [Homo sapiens] GI:23345324;
           contains Pfam profile PF01569: PAP2 superfamily
          Length = 416

 Score = 27.1 bits (57), Expect = 9.8
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = -2

Query: 416 SSGHFQTLYKFLYLPYFDISIGRRFFRH 333
           S G+ Q L +FL    FDI  G RFF++
Sbjct: 369 SVGYLQKLCEFLSHDSFDIDTGIRFFQY 396


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,997,578
Number of Sequences: 28952
Number of extensions: 257808
Number of successful extensions: 605
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 602
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1226538000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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