Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0302.Seq
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 168 5e-43
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 168 5e-43
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 82 5e-17
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 79 6e-16
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 43 4e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 8e-04
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 29 0.52
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 27 2.1
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 27 2.8
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 6.4
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 6.4
SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po... 25 8.5
SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch... 25 8.5
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 168 bits (409), Expect = 5e-43
Identities = 93/177 (52%), Positives = 116/177 (65%), Gaps = 1/177 (0%)
Frame = +2
Query: 2 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 181
LVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522
Query: 182 EESGEHIVAGAGELHLEICLKDLEGTMLAFQSRSLTLSCRTVRP*LRNRTSSVSQSR-PT 358
ESGEHIVAGAGELHLEICLKDL+ + ++ + R + +S + S+ P
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQEDHAGIPLK-ISPPVVSYRESVSEPSSMTALSKSPN 581
Query: 359 STTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAXAP 529
+PM + L I+ G VNPRDDFK AR + +++ + VT+A KI P
Sbjct: 582 KHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGP 638
Score = 70.9 bits (166), Expect = 1e-13
Identities = 50/118 (42%), Positives = 68/118 (57%), Gaps = 3/118 (2%)
Frame = +1
Query: 256 DHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQAHA*WSARGH**GSRE 435
DHA IP+K S PVVSYRE+V+E S LSKSPNKHNR+FM A+ + + G
Sbjct: 548 DHAGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETG-HV 606
Query: 436 SPR*LQDLRS-VSYRKVRIXCYRSPEDWCFXPEGTGPTSWWIAQRSSV--LNEIKDSV 600
+PR +R+ + + + + WCF P+ TG + + Q +V LNEIKDSV
Sbjct: 607 NPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTG-ANVVVDQTKAVAYLNEIKDSV 663
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 168 bits (409), Expect = 5e-43
Identities = 93/177 (52%), Positives = 116/177 (65%), Gaps = 1/177 (0%)
Frame = +2
Query: 2 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 181
LVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN DLPKLVEGLKRL+KSDP V C
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522
Query: 182 EESGEHIVAGAGELHLEICLKDLEGTMLAFQSRSLTLSCRTVRP*LRNRTSSVSQSR-PT 358
ESGEHIVAGAGELHLEICLKDL+ + ++ + R + +S + S+ P
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQEDHAGIPLK-ISPPVVSYRESVSEPSSMTALSKSPN 581
Query: 359 STTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAXAP 529
+PM + L I+ G VNPRDDFK AR + +++ + VT+A KI P
Sbjct: 582 KHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGP 638
Score = 70.9 bits (166), Expect = 1e-13
Identities = 50/118 (42%), Positives = 68/118 (57%), Gaps = 3/118 (2%)
Frame = +1
Query: 256 DHACIPIKKSDPVVSYRETVAEESDQLCLSKSPNKHNRLFMKAQAHA*WSARGH**GSRE 435
DHA IP+K S PVVSYRE+V+E S LSKSPNKHNR+FM A+ + + G
Sbjct: 548 DHAGIPLKISPPVVSYRESVSEPSSMTALSKSPNKHNRIFMTAEPMSEELSVAIETG-HV 606
Query: 436 SPR*LQDLRS-VSYRKVRIXCYRSPEDWCFXPEGTGPTSWWIAQRSSV--LNEIKDSV 600
+PR +R+ + + + + WCF P+ TG + + Q +V LNEIKDSV
Sbjct: 607 NPRDDFKVRARIMADEFGWDVTDARKIWCFGPDTTG-ANVVVDQTKAVAYLNEIKDSV 663
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 82.2 bits (194), Expect = 5e-17
Identities = 38/83 (45%), Positives = 56/83 (67%)
Frame = +2
Query: 2 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 181
+++T T+ + N N+ + + P+VRVA+EP P ++ KLV GL L ++DP VQ
Sbjct: 538 VLRTATLCSSPNGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAV 597
Query: 182 EESGEHIVAGAGELHLEICLKDL 250
EE+GEH++ AGE+HLE CLKDL
Sbjct: 598 EENGEHVIMCAGEIHLERCLKDL 620
Score = 25.4 bits (53), Expect = 6.4
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +1
Query: 262 ACIPIKKSDPVVSYRETVAEESDQLCLSK 348
A I I+ S P+V YRET D L +K
Sbjct: 625 AKIEIQASQPLVPYRETTIATPDLLAKNK 653
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 78.6 bits (185), Expect = 6e-16
Identities = 44/151 (29%), Positives = 74/151 (49%)
Frame = +2
Query: 77 VVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEG 256
V +VAVEP NP++LPKL++GL++ KS P+ EESGEH + G GE++++ L DL
Sbjct: 600 VFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRT 659
Query: 257 TMLAFQSRSLTLSCRTVRP*LRNRTSSVSQSRPTSTTVYS*RLKPMPDGLPEDIDEGRVN 436
+ R R + + P + ++P+ G+ DI+ G+VN
Sbjct: 660 LYSEIEIRVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDIENGKVN 719
Query: 437 PRDDFKTCARYLTEKYEYXVTEAPKIGAXAP 529
K + + + Y++ + + I A P
Sbjct: 720 INWPQKRISEFFQKNYDWDLLASRSIWAFGP 750
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 42.7 bits (96), Expect = 4e-05
Identities = 25/74 (33%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = +2
Query: 17 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESG 193
T T ++ M M F PV+ ++++PK+ D + L R + DP + ++ ES
Sbjct: 456 TFTDGSVSYTMTSM-FVPEPVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESK 513
Query: 194 EHIVAGAGELHLEI 235
E I++G GELHLE+
Sbjct: 514 ETIISGMGELHLEV 527
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 38.3 bits (85), Expect = 8e-04
Identities = 18/60 (30%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
Frame = +2
Query: 74 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDL 250
PV ++EP + D P L+E L + + DP + + E+G+ ++ G G +HL++ + L
Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERL 502
>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 241
Score = 29.1 bits (62), Expect = 0.52
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
Frame = +2
Query: 107 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 235
P D+ KL EGL++L + D +VQ +NE + GE H+++
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 27.1 bits (57), Expect = 2.1
Identities = 26/89 (29%), Positives = 34/89 (38%)
Frame = +2
Query: 206 AGAGELHLEICLKDLEGTMLAFQSRSLTLSCRTVRP*LRNRTSSVSQSRPTSTTVYS*RL 385
AG G + L + L RS + S R+ RP R S S P++
Sbjct: 323 AGVGSVPLPAPTSS-QSLRLGSLHRSRSPSPRSGRP--RRSPSPSHLSIPSTLPPADGVP 379
Query: 386 KPMPDGLPEDIDEGRVNPRDDFKTCARYL 472
KP PDG P + P D K +R L
Sbjct: 380 KPTPDGFPRRFERDPTIPPDSIKVYSRTL 408
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 26.6 bits (56), Expect = 2.8
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 380 RLKPMPDGLPEDIDEGRVNPRDDFKT 457
R K LPE IDEG+++ DD KT
Sbjct: 186 RAKLRDPTLPEKIDEGKLSDVDDGKT 211
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.4 bits (53), Expect = 6.4
Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = -1
Query: 222 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 130
S+ +P+T+CSPD+++ ++ G L ++ P+
Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.4 bits (53), Expect = 6.4
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +2
Query: 317 LRNRTSSVSQSRPTSTTVYS*RLKP 391
L N T+ + ++ PT+TT+ + R KP
Sbjct: 1683 LENATNDLKETFPTTTTISTARAKP 1707
>SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1057
Score = 25.0 bits (52), Expect = 8.5
Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 1/59 (1%)
Frame = +2
Query: 227 LEICLKDLEGTMLAFQSRSLTLSCRTVRP*LRNRTSSVSQSRPTSTTVYS*RL-KPMPD 400
LE C+KDL T S+ L+ + P L+ + SQS T + + L K +PD
Sbjct: 232 LEACIKDLSATTFVATVESVALA--VLVPILKRALAQRSQSMLRLTVIITDNLCKLVPD 288
>SPBC3F6.01c |||serine/threonine protein phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 25.0 bits (52), Expect = 8.5
Identities = 15/59 (25%), Positives = 26/59 (44%), Gaps = 1/59 (1%)
Frame = -1
Query: 294 DRVRLLDWNASMVPSRS-LRQISRWSSPAPATMCSPDSSLIHCTMGSDLARRLRPSTSL 121
D++R +D + P +S L W+ P PA P + G D+++R + L
Sbjct: 339 DQLRNIDRFSKKQPGQSGLMMEMLWTDPQPAPGRGPSKRGVGLQFGPDVSKRFCEANGL 397
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,527,714
Number of Sequences: 5004
Number of extensions: 51438
Number of successful extensions: 182
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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