Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0302.Seq
(600 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 159 1e-39
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 91 5e-19
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 91 5e-19
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 91 5e-19
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 87 8e-18
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 58 6e-09
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 42 2e-04
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 42 2e-04
At3g59420.1 68416.m06627 receptor protein kinase, putative (ACR4... 30 1.0
At2g47500.1 68415.m05929 kinesin motor protein-related 30 1.0
At1g44120.1 68414.m05096 C2 domain-containing protein / armadill... 30 1.0
At3g03405.1 68416.m00338 hypothetical protein temporary automate... 28 5.4
At1g53780.1 68414.m06120 26S proteasome AAA-ATPase subunit, puta... 28 5.4
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 27 7.2
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 27 9.5
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 27 9.5
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 159 bits (387), Expect = 1e-39
Identities = 94/201 (46%), Positives = 122/201 (60%), Gaps = 4/201 (1%)
Frame = +2
Query: 2 LVKTGTITTFK--NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC 175
+ K T+T K +AH ++ MKFSVSPVVRVAV+ K +DLPKLVEGLKRLAKSDPMV C
Sbjct: 461 ITKNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVC 520
Query: 176 INEESGEHIVAGAGELHLEICLKDLEGTMLAFQSRSLTLSCRTVRP*LRNR-TSSVSQSR 352
EESGEHIVAGAGELHLEICLKDL+ + + + R + +R T +V
Sbjct: 521 TMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVCDRSTRTVMSKS 580
Query: 353 PTSTTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAXAPR 532
P +PM +GL E ID+GR+ PRDD K ++ L E++ + A KI A P
Sbjct: 581 PNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPE 640
Query: 533 VPAQH-PGGLLKGVQSLMKLR 592
+ + KGVQ L +++
Sbjct: 641 TTGPNMVVDMCKGVQYLNEIK 661
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 91.1 bits (216), Expect = 5e-19
Identities = 56/182 (30%), Positives = 93/182 (51%), Gaps = 6/182 (3%)
Frame = +2
Query: 2 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 172
++KT T+ ++ + + +KF+ PVV+ A EP NP++LPK+VEGL++++KS P+
Sbjct: 559 IMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 618
Query: 173 CINEESGEHIVAGAGELHLEICLKDLEGTMLAFQSR---SLTLSCRTVRP*LRNRTSSVS 343
EESGEH + G GEL+L+ +KDL Q + + C TV + + +
Sbjct: 619 TKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCETV---VESSSMKCF 675
Query: 344 QSRPTSTTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAX 523
P + +P+ GL EDI+ G V+ + + KY++ + A I A
Sbjct: 676 AETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAF 735
Query: 524 AP 529
P
Sbjct: 736 GP 737
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.1 bits (216), Expect = 5e-19
Identities = 55/182 (30%), Positives = 94/182 (51%), Gaps = 6/182 (3%)
Frame = +2
Query: 2 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 172
++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS P+
Sbjct: 573 IMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 632
Query: 173 CINEESGEHIVAGAGELHLEICLKDLEGTMLAFQSR---SLTLSCRTVRP*LRNRTSSVS 343
EESGEH + G GEL+L+ +KDL + + + C TV + + +
Sbjct: 633 TKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV---VESSSMKCF 689
Query: 344 QSRPTSTTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAX 523
P + +P+ GL EDI+ G V+ + K + KY++ + A I A
Sbjct: 690 AETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAF 749
Query: 524 AP 529
P
Sbjct: 750 GP 751
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 91.1 bits (216), Expect = 5e-19
Identities = 55/182 (30%), Positives = 94/182 (51%), Gaps = 6/182 (3%)
Frame = +2
Query: 2 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 172
++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS P+
Sbjct: 573 IMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 632
Query: 173 CINEESGEHIVAGAGELHLEICLKDLEGTMLAFQSR---SLTLSCRTVRP*LRNRTSSVS 343
EESGEH + G GEL+L+ +KDL + + + C TV + + +
Sbjct: 633 TKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV---VESSSMKCF 689
Query: 344 QSRPTSTTVYS*RLKPMPDGLPEDIDEGRVNPRDDFKTCARYLTEKYEYXVTEAPKIGAX 523
P + +P+ GL EDI+ G V+ + K + KY++ + A I A
Sbjct: 690 AETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAF 749
Query: 524 AP 529
P
Sbjct: 750 GP 751
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 87.0 bits (206), Expect = 8e-18
Identities = 37/82 (45%), Positives = 59/82 (71%)
Frame = +2
Query: 8 KTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEE 187
K+ T+++ +N + M+F VSP +RVA+EP +PAD+ L++GL+ L ++DP V+
Sbjct: 515 KSATLSSTRNCWPLASMEFQVSPTLRVAIEPSDPADMSALMKGLRLLNRADPFVEITVSA 574
Query: 188 SGEHIVAGAGELHLEICLKDLE 253
GEH++A AGE+HLE C+KDL+
Sbjct: 575 RGEHVLAAAGEVHLERCVKDLK 596
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 57.6 bits (133), Expect = 6e-09
Identities = 31/80 (38%), Positives = 49/80 (61%), Gaps = 1/80 (1%)
Frame = +2
Query: 17 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ-CINEESG 193
T++ +N ++ M F PV++VA+EPK AD+ K+ GL +LA+ DP +EE
Sbjct: 475 TLSDPENPVVLERMDFP-DPVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMN 533
Query: 194 EHIVAGAGELHLEICLKDLE 253
+ ++ G GELHLEI + L+
Sbjct: 534 QTVIEGMGELHLEIIVDRLK 553
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 42.3 bits (95), Expect = 2e-04
Identities = 21/55 (38%), Positives = 34/55 (61%), Gaps = 1/55 (1%)
Frame = +2
Query: 74 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEI 235
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDI 515
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 42.3 bits (95), Expect = 2e-04
Identities = 21/55 (38%), Positives = 34/55 (61%), Gaps = 1/55 (1%)
Frame = +2
Query: 74 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEI 235
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDI 515
>At3g59420.1 68416.m06627 receptor protein kinase, putative (ACR4)
identical to putative receptor protein kinase ACR4
[Arabidopsis thaliana] GI:20302590; contains protein
kinase domain, Pfam:PF00069
Length = 895
Score = 30.3 bits (65), Expect = 1.0
Identities = 13/31 (41%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +1
Query: 253 GDHACIPIKKSDPVVSYRETV-AEESDQLCL 342
G H C+P S P Y+++V E SDQ+C+
Sbjct: 366 GSHICLPCSTSCPPGMYQKSVCTERSDQVCV 396
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 30.3 bits (65), Expect = 1.0
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +2
Query: 32 KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGL 139
+N N S+S +VR + K P D+PKL+E L
Sbjct: 218 ENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At1g44120.1 68414.m05096 C2 domain-containing protein /
armadillo/beta-catenin repeat family protein similar to
CCLS 65 [Silene latifolia] GI:2570102; contains Pfam
profiles PF00514: Armadillo/beta-catenin-like repeat,
PF00168: C2 domain
Length = 2114
Score = 30.3 bits (65), Expect = 1.0
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = -1
Query: 267 ASMVPSRSLRQISRWSSPAPATMCSPDSSLIHCTMGSDLARRLRPSTSLGRSAGF 103
A +P L S+ SPAP++ +SL++C GS L ++ +L RS F
Sbjct: 1958 ADAIPVLQLMMKSKLKSPAPSSFHERGNSLLNCLPGS-LTVAIKRGDNLKRSNAF 2011
>At3g03405.1 68416.m00338 hypothetical protein temporary automated
functional assignment
Length = 193
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 303 VRHDRVRLLDWNASMVPSRSLRQISRWSSPAPATMC 196
VR D RL+ WN + +RS++ + W + + A C
Sbjct: 27 VRGDNTRLVVWNPYIGQTRSIKLVKGWDTCSYAFGC 62
>At1g53780.1 68414.m06120 26S proteasome AAA-ATPase subunit,
putative similar to 26S proteasome AAA-ATPase subunit
RPT1 SP:Q41365 from [Spinacia oleracea]
Length = 464
Score = 27.9 bits (59), Expect = 5.4
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 227 LEICLKDLEGTMLAFQSRSLTLSC-RTVR 310
+E CL DLEG F+ + T+SC R +R
Sbjct: 371 VEFCLPDLEGRTQIFKIHTRTMSCERDIR 399
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/45 (31%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Frame = +2
Query: 74 PVVRVAVEPKNPADLPKLVEGLKRLAK-SDPMVQCINEESGEHIV 205
P+++ V P N AD+ VE +K + S+ + +C+ EE E+++
Sbjct: 63 PIIKKVVVP-NRADIKTSVEEVKAIIDDSEALYECLIEEGEEYLL 106
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 83 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGE 220
+VA+ +P D+ K+ E L RLA D + +S E + G +
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSK 173
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +2
Query: 83 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGE 220
+VA+ +P D+ K+ E L RLA D + +S E + G +
Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSK 173
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,555,026
Number of Sequences: 28952
Number of extensions: 282629
Number of successful extensions: 865
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 832
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 854
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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