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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0300.Seq
         (595 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p...   133   2e-32
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po...    30   0.29 
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc...    29   0.39 
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca...    28   1.2  
SPBP22H7.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   6.3  
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    25   8.3  
SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po...    25   8.3  
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   8.3  

>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 525

 Score =  133 bits (322), Expect = 2e-32
 Identities = 70/102 (68%), Positives = 79/102 (77%), Gaps = 1/102 (0%)
 Frame = -3

Query: 551 QAIYVPADDL-QILLRYHFCSLGCYH*LSRAIAELGIYPAVDPLDSTSRIMDPNIIGAEH 375
           QA+YVPADDL        F  L     LSR+I+ELGIYPAVDPLDS SR+MDP I+G EH
Sbjct: 354 QAVYVPADDLTDPAPATTFAHLDATTVLSRSISELGIYPAVDPLDSKSRMMDPRILGEEH 413

Query: 374 YNVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVARAVK 249
           YN+A  VQ++LQ+YKSLQDIIAILGMDELSE DKLTV RA K
Sbjct: 414 YNLAGSVQQMLQEYKSLQDIIAILGMDELSEADKLTVERARK 455



 Score = 89.0 bits (211), Expect = 5e-19
 Identities = 41/67 (61%), Positives = 53/67 (79%)
 Frame = -2

Query: 252 KIQRFLSQPFQVAEVFTGHAGKLVPLEETIKGFSKILAGDYDHLPEVAFYMVGPIEEVVA 73
           K+QRFLSQPF VAEVFTG  G+LV L++TI+ F +IL G +D LPE AFYMVG I++ V 
Sbjct: 455 KVQRFLSQPFAVAEVFTGIEGRLVSLKDTIRSFKEILEGKHDSLPESAFYMVGSIDDAVK 514

Query: 72  KADTLAK 52
           KA+ +A+
Sbjct: 515 KAEKIAQ 521


>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 619

 Score = 29.9 bits (64), Expect = 0.29
 Identities = 14/38 (36%), Positives = 25/38 (65%), Gaps = 1/38 (2%)
 Frame = -3

Query: 374 YNVARG-VQKILQDYKSLQDIIAILGMDELSEEDKLTV 264
           +N  R  +++I+Q   S+ +II ++G   LSE DK+T+
Sbjct: 480 FNTLRDQIKQIIQQEDSMLEIIQLVGKSALSETDKVTL 517


>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 503

 Score = 29.5 bits (63), Expect = 0.39
 Identities = 20/80 (25%), Positives = 39/80 (48%), Gaps = 4/80 (5%)
 Frame = -3

Query: 473 LSRAIAELGIYPAVDPLDSTSRIMDPNI----IGAEHYNVARGVQKILQDYKSLQDIIAI 306
           + R +    IYP ++ L S SR+M   I       +H +V+  +  +    +    + ++
Sbjct: 357 VDRQLHNNAIYPPINVLPSLSRLMKSAIGEGMTRNDHGDVSNQLYAMYAIGRDAASMKSV 416

Query: 305 LGMDELSEEDKLTVARAVKF 246
           +G + LS+ED+L +    KF
Sbjct: 417 VGEEALSQEDRLALEFLGKF 436


>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
           synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 2410

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 15/57 (26%), Positives = 26/57 (45%)
 Frame = -3

Query: 488 GCYH*LSRAIAELGIYPAVDPLDSTSRIMDPNIIGAEHYNVARGVQKILQDYKSLQD 318
           GCY+  + +   + + PA      +   +D   I + H NV R +  I + Y SL +
Sbjct: 529 GCYNGSATSYPTVDLSPAQRGCQDSWNYLDHFDIASAHRNVYRNIHSIRRHYLSLSE 585


>SPBP22H7.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 17/64 (26%), Positives = 27/64 (42%), Gaps = 1/64 (1%)
 Frame = -1

Query: 529 MTCRSCSATTFAHLDATTDSPEPLLNWVSTQLWILLTQLPVSW-TPILLELSTTMLHVEF 353
           M    C  T+FA +D      E   NWV    +  L+ +  ++ TP L    T +L +  
Sbjct: 109 MLINVCVITSFASVDIYRAKDEIFANWVDMDYYEKLSYIGSAFITPALYFTLTLILFLPR 168

Query: 352 RKFF 341
           R  +
Sbjct: 169 RNIY 172


>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 25.0 bits (52), Expect = 8.3
 Identities = 11/33 (33%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
 Frame = -1

Query: 583  YHHQEGSITSDRLFMYQ---LMTCRSCSATTFA 494
            +   E ++T D+ F+     L+ C SC+AT+F+
Sbjct: 1233 FQRYESTLTDDQCFINVSPLLLKCPSCNATSFS 1265


>SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 449

 Score = 25.0 bits (52), Expect = 8.3
 Identities = 11/44 (25%), Positives = 25/44 (56%)
 Frame = -3

Query: 431 DPLDSTSRIMDPNIIGAEHYNVARGVQKILQDYKSLQDIIAILG 300
           + +D+T +  +P  I  E   V +  Q ++ + + ++DI++ LG
Sbjct: 371 EEIDATIQTYNPQNIDLEDEAVEKEWQDLVAEEQKVEDIVSTLG 414


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 25.0 bits (52), Expect = 8.3
 Identities = 17/72 (23%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
 Frame = -1

Query: 529 MTCRSCSATTFAHLDATTDSPEPLLNW-VSTQLWILLTQLPVSWTPILLELSTTMLHVEF 353
           +T  +C+ +T     +T  +  PL     +T   +  T  PV+ TP+     TT   + +
Sbjct: 422 LTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTTTNCTTSTSIPY 481

Query: 352 RKFFRTTNPCRT 317
                T+ P  T
Sbjct: 482 TSTPVTSTPLTT 493


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,535,781
Number of Sequences: 5004
Number of extensions: 52398
Number of successful extensions: 162
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 258201856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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