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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0296.Seq
         (598 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha...    35   0.047
At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha...    35   0.047

>At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           similar to SP|P36967 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Dictyostelium discoideum}; contains Pfam profiles
           PF00549: CoA-ligase, PF02629: CoA binding domain
          Length = 341

 Score = 34.7 bits (76), Expect = 0.047
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = -1

Query: 586 PPGXRMGHAGAIISAAKEAPWTKLR 512
           PPG RMGHAGAI+S  K     K++
Sbjct: 286 PPGRRMGHAGAIVSGGKGTAQDKIK 310



 Score = 29.5 bits (63), Expect = 1.8
 Identities = 15/29 (51%), Positives = 16/29 (55%)
 Frame = -3

Query: 596 LXAPPGSTNGSRWCHHFGGKGGAMDKIKA 510
           L APPG   G       GGKG A DKIK+
Sbjct: 283 LTAPPGRRMGHAGAIVSGGKGTAQDKIKS 311


>At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial, putative / succinyl-CoA
           synthetase, alpha chain, putative / SCS-alpha, putative
           identical to SP|P53586 Succinyl-CoA ligase [GDP-forming]
           alpha-chain, mitochondrial precursor (EC 6.2.1.4)
           (Succinyl-CoA synthetase, alpha chain) (SCS-alpha)
           {Arabidopsis thaliana}; strong similarity to SP|P13086
           Succinyl-CoA ligase [GDP-forming] alpha-chain,
           mitochondrial precursor {Rattus norvegicus}; contains
           Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding
           domain
          Length = 347

 Score = 34.7 bits (76), Expect = 0.047
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = -1

Query: 586 PPGXRMGHAGAIISAAKEAPWTKLR 512
           PPG RMGHAGAI+S  K     K++
Sbjct: 291 PPGRRMGHAGAIVSGGKGTAQDKIK 315



 Score = 29.5 bits (63), Expect = 1.8
 Identities = 15/29 (51%), Positives = 16/29 (55%)
 Frame = -3

Query: 596 LXAPPGSTNGSRWCHHFGGKGGAMDKIKA 510
           L APPG   G       GGKG A DKIK+
Sbjct: 288 LTAPPGRRMGHAGAIVSGGKGTAQDKIKS 316


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,319,780
Number of Sequences: 28952
Number of extensions: 235417
Number of successful extensions: 359
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 351
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 359
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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