BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0296.Seq (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 35 0.047 At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 35 0.047 >At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial, putative / succinyl-CoA synthetase, alpha chain, putative / SCS-alpha, putative similar to SP|P36967 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) {Dictyostelium discoideum}; contains Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding domain Length = 341 Score = 34.7 bits (76), Expect = 0.047 Identities = 14/25 (56%), Positives = 17/25 (68%) Frame = -1 Query: 586 PPGXRMGHAGAIISAAKEAPWTKLR 512 PPG RMGHAGAI+S K K++ Sbjct: 286 PPGRRMGHAGAIVSGGKGTAQDKIK 310 Score = 29.5 bits (63), Expect = 1.8 Identities = 15/29 (51%), Positives = 16/29 (55%) Frame = -3 Query: 596 LXAPPGSTNGSRWCHHFGGKGGAMDKIKA 510 L APPG G GGKG A DKIK+ Sbjct: 283 LTAPPGRRMGHAGAIVSGGKGTAQDKIKS 311 >At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial, putative / succinyl-CoA synthetase, alpha chain, putative / SCS-alpha, putative identical to SP|P53586 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) {Arabidopsis thaliana}; strong similarity to SP|P13086 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor {Rattus norvegicus}; contains Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding domain Length = 347 Score = 34.7 bits (76), Expect = 0.047 Identities = 14/25 (56%), Positives = 17/25 (68%) Frame = -1 Query: 586 PPGXRMGHAGAIISAAKEAPWTKLR 512 PPG RMGHAGAI+S K K++ Sbjct: 291 PPGRRMGHAGAIVSGGKGTAQDKIK 315 Score = 29.5 bits (63), Expect = 1.8 Identities = 15/29 (51%), Positives = 16/29 (55%) Frame = -3 Query: 596 LXAPPGSTNGSRWCHHFGGKGGAMDKIKA 510 L APPG G GGKG A DKIK+ Sbjct: 288 LTAPPGRRMGHAGAIVSGGKGTAQDKIKS 316 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,319,780 Number of Sequences: 28952 Number of extensions: 235417 Number of successful extensions: 359 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 351 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 359 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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